CLE20_ARATH
ID CLE20_ARATH Reviewed; 83 AA.
AC Q3EDI6;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 20 {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE20p {ECO:0000303|PubMed:16489133};
DE Flags: Precursor;
GN Name=CLE20 {ECO:0000303|PubMed:16489133};
GN OrderedLocusNames=At1g05065 {ECO:0000312|Araport:AT1G05065};
GN ORFNames=T7A14 {ECO:0000312|EMBL:AC005322};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [6]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [7]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [8]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [9]
RP DEVELOPMENTAL STAGE, AND FUNCTION.
RX PubMed=20697738; DOI=10.1007/s00425-010-1236-4;
RA Meng L., Feldman L.J.;
RT "CLE14/CLE20 peptides may interact with CLAVATA2/CORYNE receptor-like
RT kinases to irreversibly inhibit cell division in the root meristem of
RT Arabidopsis.";
RL Planta 232:1061-1074(2010).
RN [10]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE20p]: Extracellular signal peptide that regulates cell
CC fate. Represses root apical meristem maintenance. Inhibits irreversibly
CC root growth by reducing cell division rates in the root apical meristem
CC (PubMed:20697738). Regulates the transition of protophloem cells from
CC proliferation to differentiation, thus impinging on postembryonic
CC growth capacity of the root meristem; this signaling pathway requires
CC CRN and CLV2 (PubMed:28607033). {ECO:0000269|PubMed:16902140,
CC ECO:0000269|PubMed:20697738, ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE20p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE20p]: Mostly expressed in roots, seedlings,
CC leaves, flowers, stems and apex, and, to a lower extent, in siliques
CC and pollen. {ECO:0000269|PubMed:12602871}.
CC -!- DEVELOPMENTAL STAGE: [CLE20p]: Expressed specifically in
CC differentiating cells of the root. {ECO:0000269|PubMed:20697738}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AC005322; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE27786.1; -; Genomic_DNA.
DR RefSeq; NP_001318924.1; NM_001331517.1.
DR AlphaFoldDB; Q3EDI6; -.
DR SMR; Q3EDI6; -.
DR STRING; 3702.AT1G05065.1; -.
DR PaxDb; Q3EDI6; -.
DR PRIDE; Q3EDI6; -.
DR EnsemblPlants; AT1G05065.1; AT1G05065.1; AT1G05065.
DR GeneID; 28716239; -.
DR Gramene; AT1G05065.1; AT1G05065.1; AT1G05065.
DR KEGG; ath:AT1G05065; -.
DR Araport; AT1G05065; -.
DR TAIR; locus:504956102; AT1G05065.
DR HOGENOM; CLU_199297_0_0_1; -.
DR InParanoid; Q3EDI6; -.
DR OMA; IHVERQR; -.
DR OrthoDB; 1608849at2759; -.
DR PhylomeDB; Q3EDI6; -.
DR PRO; PR:Q3EDI6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q3EDI6; baseline and differential.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; ISS:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..83
FT /note="CLAVATA3/ESR (CLE)-related protein 20"
FT /id="PRO_0000401269"
FT PEPTIDE 70..81
FT /note="CLE20p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401270"
SQ SEQUENCE 83 AA; 9538 MW; FAFE22EA5FA90F39 CRC64;
MKNKNMNPSR PRLLCLIVFL FLVIVLSKAS RIHVERRRFS SKPSGENREF LPSQPTFPVV
DAGEILPDKR KVKTGSNPLH NKR
