CLE21_ARATH
ID CLE21_ARATH Reviewed; 106 AA.
AC Q9LV97;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 21 {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE21p {ECO:0000303|PubMed:16489133};
DE Flags: Precursor;
GN Name=CLE21 {ECO:0000303|PubMed:16489133};
GN OrderedLocusNames=At5g64800 {ECO:0000312|Araport:AT5G64800};
GN ORFNames=MXK3.2 {ECO:0000312|EMBL:BAA97292.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [5]
RP FUNCTION.
RX PubMed=16407446; DOI=10.1104/pp.105.072678;
RA Ni J., Clark S.E.;
RT "Evidence for functional conservation, sufficiency, and proteolytic
RT processing of the CLAVATA3 CLE domain.";
RL Plant Physiol. 140:726-733(2006).
RN [6]
RP FUNCTION, AND GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [7]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [8]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [9]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [10]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE21p]: Extracellular signal peptide that regulates cell
CC fate. Represses root apical meristem maintenance. Regulates the
CC transition of protophloem cells from proliferation to differentiation,
CC thus impinging on postembryonic growth capacity of the root meristem;
CC this signaling pathway requires CRN and CLV2 (PubMed:28607033).
CC {ECO:0000269|PubMed:16407446, ECO:0000269|PubMed:16489133,
CC ECO:0000269|PubMed:16902140, ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE21p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE21p]: Mostly expressed in leaves and apex, and,
CC to a lower extent, in seedlings, flowers, stems and siliques.
CC {ECO:0000269|PubMed:12602871}.
CC -!- PTM: [CLE21p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-100 enhances binding affinity of the CLE21p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AB019236; BAA97292.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97952.1; -; Genomic_DNA.
DR RefSeq; NP_001318877.1; NM_001345649.1.
DR AlphaFoldDB; Q9LV97; -.
DR STRING; 3702.AT5G64800.1; -.
DR PaxDb; Q9LV97; -.
DR PRIDE; Q9LV97; -.
DR EnsemblPlants; AT5G64800.1; AT5G64800.1; AT5G64800.
DR GeneID; 28721288; -.
DR Gramene; AT5G64800.1; AT5G64800.1; AT5G64800.
DR KEGG; ath:AT5G64800; -.
DR Araport; AT5G64800; -.
DR TAIR; locus:2177629; AT5G64800.
DR HOGENOM; CLU_176520_0_0_1; -.
DR OMA; EMSSFNK; -.
DR OrthoDB; 1633781at2759; -.
DR PRO; PR:Q9LV97; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LV97; baseline and differential.
DR Genevisible; Q9LV97; AT.
DR GO; GO:0048046; C:apoplast; ISM:TAIR.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR InterPro; IPR033249; CLE_plant.
DR PANTHER; PTHR34545; PTHR34545; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..106
FT /note="CLAVATA3/ESR (CLE)-related protein 21"
FT /id="PRO_0000401271"
FT PEPTIDE 94..105
FT /note="CLE21p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401272"
FT REGION 72..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 97
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 100
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 100
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 106 AA; 12377 MW; A85ECD52EF507B4C CRC64;
MLILSSRYAM KRDVLIIVIF TVLVLIIISR SSSIQAGRFM TTGRNRNLSV ARSLYYKNHH
KVVITEMSNF NKVRRRSSRF RRKTDGDEEE EEKRSIPTGP NPLHNK
