CLE25_ARATH
ID CLE25_ARATH Reviewed; 81 AA.
AC Q8LFL4;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 25 {ECO:0000303|PubMed:16489133};
DE Contains:
DE RecName: Full=CLE25p {ECO:0000303|PubMed:16489133};
DE Flags: Precursor;
GN Name=CLE25 {ECO:0000303|PubMed:16489133};
GN OrderedLocusNames=At3g28455 {ECO:0000312|Araport:AT3G28455};
GN ORFNames=MFJ20 {ECO:0000312|EMBL:AB026644};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [6]
RP FUNCTION, AND GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [7]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [8]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [9]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [10]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE25p]: Extracellular signal peptide that regulates cell
CC fate. Represses root apical meristem maintenance. Regulates the
CC transition of protophloem cells from proliferation to differentiation,
CC thus impinging on postembryonic growth capacity of the root meristem;
CC this signaling pathway requires CRN and CLV2 (PubMed:28607033).
CC {ECO:0000269|PubMed:16489133, ECO:0000269|PubMed:16902140,
CC ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE25p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE25p]: Mostly expressed in flowers and siliques,
CC and, to a lower extent, in roots, stems, apex, seedlings, leaves and
CC pollen. {ECO:0000269|PubMed:12602871}.
CC -!- PTM: [CLE25p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-64 enhances binding affinity of the CLE25p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AB026644; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002686; AEE77447.1; -; Genomic_DNA.
DR EMBL; AY084776; AAM61344.1; -; mRNA.
DR RefSeq; NP_683600.1; NM_148758.2.
DR AlphaFoldDB; Q8LFL4; -.
DR SMR; Q8LFL4; -.
DR STRING; 3702.AT3G28455.1; -.
DR PaxDb; Q8LFL4; -.
DR PRIDE; Q8LFL4; -.
DR EnsemblPlants; AT3G28455.1; AT3G28455.1; AT3G28455.
DR GeneID; 822475; -.
DR Gramene; AT3G28455.1; AT3G28455.1; AT3G28455.
DR KEGG; ath:AT3G28455; -.
DR Araport; AT3G28455; -.
DR TAIR; locus:504955760; AT3G28455.
DR eggNOG; ENOG502R1RB; Eukaryota.
DR HOGENOM; CLU_163620_0_0_1; -.
DR InParanoid; Q8LFL4; -.
DR OMA; WDSSFIY; -.
DR OrthoDB; 1555873at2759; -.
DR PhylomeDB; Q8LFL4; -.
DR PRO; PR:Q8LFL4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LFL4; baseline and differential.
DR GO; GO:0048046; C:apoplast; ISM:TAIR.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; IPI:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0010116; P:positive regulation of abscisic acid biosynthetic process; IGI:TAIR.
DR GO; GO:1902584; P:positive regulation of response to water deprivation; IMP:TAIR.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR InterPro; IPR039316; CLE25/26.
DR PANTHER; PTHR34277; PTHR34277; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..81
FT /note="CLAVATA3/ESR (CLE)-related protein 25"
FT /id="PRO_0000401275"
FT PEPTIDE 58..69
FT /note="CLE25p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401276"
FT REGION 57..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 61
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 64
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 64
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 81 AA; 8625 MW; DFDDE5BDBF360E8D CRC64;
MGGNGIRALV GVIASLGLIV FLLVGILANS APSVPSSENV KTLRFSGKDV NLFHVSKRKV
PNGPDPIHNR KAETSRRPPR V
