CLE2_ARATH
ID CLE2_ARATH Reviewed; 75 AA.
AC O49519;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 2 {ECO:0000303|PubMed:18034320};
DE Contains:
DE RecName: Full=CLE2p {ECO:0000303|PubMed:18034320};
DE Flags: Precursor;
GN Name=CLE2 {ECO:0000303|PubMed:18034320};
GN OrderedLocusNames=At4g18510 {ECO:0000312|Araport:AT4G18510};
GN ORFNames=F28J12.170 {ECO:0000312|EMBL:CAA16731.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [6]
RP FUNCTION, AND GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [7]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [8]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [9]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY OF CLE2P, INTERACTION WITH
RP CLV1, GLYCOSYLATION AT PRO-70, HYDROXYLATION AT PRO-67 AND PRO-70, AND
RP SUBCELLULAR LOCATION.
RX PubMed=19525968; DOI=10.1038/nchembio.182;
RA Ohyama K., Shinohara H., Ogawa-Ohnishi M., Matsubayashi Y.;
RT "A glycopeptide regulating stem cell fate in Arabidopsis thaliana.";
RL Nat. Chem. Biol. 5:578-580(2009).
RN [10]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
CC -!- FUNCTION: [CLE2p]: Extracellular signal peptide that regulates cell
CC fate. May act with CLV1 as a ligand-receptor pair in a signal
CC transduction pathway, coordinating growth between adjacent meristematic
CC regions. {ECO:0000269|PubMed:16489133, ECO:0000269|PubMed:19525968}.
CC -!- SUBUNIT: [CLE2p]: Interacts with the extracellular leucine-rich repeat
CC region of CLV1. {ECO:0000269|PubMed:19525968}.
CC -!- SUBCELLULAR LOCATION: [CLE2p]: Secreted, extracellular space
CC {ECO:0000269|PubMed:19525968}.
CC -!- TISSUE SPECIFICITY: [CLE2p]: Mostly expressed in roots and seedlings,
CC and, to a lower extent, in apex. {ECO:0000269|PubMed:12602871}.
CC -!- PTM: [CLE2p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-70 enhances binding affinity of the CLE2p peptide
CC for its receptor. {ECO:0000269|PubMed:19525968}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; AL021710; CAA16731.1; -; Genomic_DNA.
DR EMBL; AL161548; CAB78853.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84056.1; -; Genomic_DNA.
DR EMBL; BT010812; AAR24179.1; -; mRNA.
DR EMBL; BT011289; AAR92325.1; -; mRNA.
DR PIR; T04547; T04547.
DR RefSeq; NP_193586.1; NM_117965.5.
DR AlphaFoldDB; O49519; -.
DR BioGRID; 12876; 2.
DR IntAct; O49519; 1.
DR STRING; 3702.AT4G18510.1; -.
DR PaxDb; O49519; -.
DR EnsemblPlants; AT4G18510.1; AT4G18510.1; AT4G18510.
DR GeneID; 827583; -.
DR Gramene; AT4G18510.1; AT4G18510.1; AT4G18510.
DR KEGG; ath:AT4G18510; -.
DR Araport; AT4G18510; -.
DR TAIR; locus:2124588; AT4G18510.
DR HOGENOM; CLU_2674476_0_0_1; -.
DR InParanoid; O49519; -.
DR OMA; DQAAGSH; -.
DR OrthoDB; 1632058at2759; -.
DR PRO; PR:O49519; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O49519; baseline and differential.
DR Genevisible; O49519; AT.
DR GO; GO:0048046; C:apoplast; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; IPI:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..75
FT /note="CLAVATA3/ESR (CLE)-related protein 2"
FT /id="PRO_0000401235"
FT PEPTIDE 64..75
FT /note="CLE2p"
FT /evidence="ECO:0000269|PubMed:19525968"
FT /id="PRO_0000401236"
FT REGION 40..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 67
FT /note="Hydroxyproline"
FT /evidence="ECO:0000269|PubMed:19525968"
FT MOD_RES 70
FT /note="Hydroxyproline"
FT /evidence="ECO:0000269|PubMed:19525968"
FT CARBOHYD 70
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000269|PubMed:19525968"
SQ SEQUENCE 75 AA; 7829 MW; 2AC4F0F2E53F98C5 CRC64;
MAKLSFTFCF LLFLLLSSIA AGSRPLEGAR VGVKVRGLSP SIEATSPTVE DDQAAGSHGK
SPERLSPGGP DPQHH
