CLE33_MEDTR
ID CLE33_MEDTR Reviewed; 75 AA.
AC A0A072V9Z0;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 1.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 33 {ECO:0000303|PubMed:31477892};
DE Short=MtCLE33 {ECO:0000303|PubMed:31477892};
DE Contains:
DE RecName: Full=CLE33p {ECO:0000305};
DE Flags: Precursor;
GN Name=CLE33 {ECO:0000303|PubMed:31477892};
GN OrderedLocusNames=MTR_2g078160 {ECO:0000312|EMBL:KEH38637.1};
GN ORFNames=MtrunA17_Chr2g0316361 {ECO:0000312|EMBL:RHN74994.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=31477892; DOI=10.1038/s41477-019-0501-1;
RA Mueller L.M., Flokova K., Schnabel E., Sun X., Fei Z., Frugoli J.,
RA Bouwmeester H.J., Harrison M.J.;
RT "A CLE-SUNN module regulates strigolactone content and fungal colonization
RT in arbuscular mycorrhiza.";
RL Nat. Plants 5:933-939(2019).
CC -!- FUNCTION: [CLE33p]: Signaling peptide involved in the regulation of
CC root colonization by arbuscular mycorrhizal (AM) fungi
CC (PubMed:31477892). Moves from root to shoot to function with the
CC receptor kinase SUNN, in a signaling pathway that repress strigolactone
CC biosynthetic genes and strigolactone content in the roots, and
CC consequently reduces the promotion of further colonization by AM fungi
CC (PubMed:31477892). {ECO:0000269|PubMed:31477892}.
CC -!- SUBCELLULAR LOCATION: [CLE33p]: Secreted, extracellular space
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: [CLE33p]: Expressed in root vasculature.
CC {ECO:0000269|PubMed:31477892}.
CC -!- INDUCTION: [CLE33p]: Induced in roots by treatment with inorganic
CC phosphate. {ECO:0000269|PubMed:31477892}.
CC -!- PTM: [CLE33p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-70 enhances binding affinity of the CLE33p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- MISCELLANEOUS: Roots of plants overexpressing CLE33 exhibit normal
CC growth, but reduced levels of colonization by arbuscular mycorrhizal
CC (AM) fungi. {ECO:0000269|PubMed:31477892}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM001218; KEH38637.1; -; Genomic_DNA.
DR EMBL; PSQE01000002; RHN74994.1; -; Genomic_DNA.
DR RefSeq; XP_013464602.1; XM_013609148.1.
DR AlphaFoldDB; A0A072V9Z0; -.
DR EnsemblPlants; KEH38637; KEH38637; MTR_2g078160.
DR Gramene; KEH38637; KEH38637; MTR_2g078160.
DR KEGG; mtr:MTR_2g078160; -.
DR HOGENOM; CLU_194792_1_0_1; -.
DR OrthoDB; 1639425at2759; -.
DR Proteomes; UP000002051; Chromosome 2.
DR Proteomes; UP000265566; Chromosome 2.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydroxylation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..75
FT /note="CLAVATA3/ESR (CLE)-related protein 33"
FT /id="PRO_5014500424"
FT PEPTIDE 64..75
FT /note="CLE33p"
FT /evidence="ECO:0000250|UniProtKB:A0A072TRR8"
FT /id="PRO_0000448636"
FT MOD_RES 67
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 70
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 70
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 75 AA; 8393 MW; CC65807DEFDC9CF1 CRC64;
MASWRMLCFV LLFTSILICH DARPLPSSLS SSNGSPAFVE SVKQVVKEIM RRKQLLGTQY
STNRLSPSGP DPHHH
