CLE43_ARATH
ID CLE43_ARATH Reviewed; 96 AA.
AC Q6IWB1; A0MDN3;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 43 {ECO:0000303|PubMed:16489133, ECO:0000303|PubMed:18034320};
DE Contains:
DE RecName: Full=CLE43p {ECO:0000303|PubMed:16489133, ECO:0000303|PubMed:18034320};
DE Flags: Precursor;
GN Name=CLE43 {ECO:0000303|PubMed:16489133, ECO:0000303|PubMed:18034320};
GN OrderedLocusNames=At1g25425 {ECO:0000312|Araport:AT1G25425};
GN ORFNames=F2J7 {ECO:0000312|EMBL:AC079281};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17229318; DOI=10.1186/1471-2164-8-18;
RA Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.;
RT "Experimental validation of novel genes predicted in the un-annotated
RT regions of the Arabidopsis genome.";
RL BMC Genomics 8:18-18(2007).
RN [6]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [7]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [8]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [9]
RP FUNCTION.
RX PubMed=23910659; DOI=10.1016/j.cub.2013.06.060;
RA Endo S., Shinohara H., Matsubayashi Y., Fukuda H.;
RT "A novel pollen-pistil interaction conferring high-temperature tolerance
RT during reproduction via CLE45 signaling.";
RL Curr. Biol. 23:1670-1676(2013).
CC -!- FUNCTION: [CLE43p]: Extracellular signal peptide that regulates cell
CC fate (By similarity). Promotes pollen tube growth prolongation in a
CC SKM1 and SKM2-dependent manner, especially under relatively high
CC temperature (at 30 degrees Celsius), thus conferring tolerance against
CC high temperature probably through the maintenance of mitochondrial
CC activity (PubMed:23910659). {ECO:0000250|UniProtKB:Q6IWA9,
CC ECO:0000269|PubMed:23910659}.
CC -!- SUBCELLULAR LOCATION: [CLE43p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE43p]: Expressed at low levels in seedlings.
CC {ECO:0000269|PubMed:16489133}.
CC -!- PTM: [CLE43p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-91 enhances binding affinity of the CLE43p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28016.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY618656; AAT36742.1; -; Genomic_DNA.
DR EMBL; AC079281; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE30624.1; -; Genomic_DNA.
DR EMBL; DQ487497; ABF59326.1; -; Genomic_DNA.
DR EMBL; DQ652649; ABK28016.1; ALT_SEQ; Genomic_DNA.
DR EMBL; EF182921; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001319079.1; NM_001332680.1.
DR AlphaFoldDB; Q6IWB1; -.
DR STRING; 3702.AT1G25425.1; -.
DR PaxDb; Q6IWB1; -.
DR PRIDE; Q6IWB1; -.
DR EnsemblPlants; AT1G25425.1; AT1G25425.1; AT1G25425.
DR GeneID; 28717267; -.
DR Gramene; AT1G25425.1; AT1G25425.1; AT1G25425.
DR KEGG; ath:AT1G25425; -.
DR Araport; AT1G25425; -.
DR TAIR; locus:1009023110; AT1G25425.
DR HOGENOM; CLU_2457917_0_0_1; -.
DR InParanoid; Q6IWB1; -.
DR OrthoDB; 1603337at2759; -.
DR PhylomeDB; Q6IWB1; -.
DR PRO; PR:Q6IWB1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6IWB1; baseline and differential.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; ISS:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR InterPro; IPR040274; CLE27/CLE43.
DR PANTHER; PTHR37184; PTHR37184; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..96
FT /note="CLAVATA3/ESR (CLE)-related protein 43"
FT /id="PRO_5000093475"
FT PEPTIDE 85..96
FT /note="CLE43p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401286"
FT REGION 71..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 88
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 91
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 91
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 96 AA; 11306 MW; CE26F4DA56BD1E75 CRC64;
MGCRDILLTF SVALLLISLF QIWLFREGRQ VPELSDDQLG KDRNTLMTSK NKNKNEDVQR
LFQRYFKGRS FGLNNTNSRF EDSNRRIPSS PDRLHN
