CLE45_ARATH
ID CLE45_ARATH Reviewed; 124 AA.
AC Q6IWA9; A0MDK4;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 45 {ECO:0000303|PubMed:16489133, ECO:0000303|PubMed:18034320};
DE Contains:
DE RecName: Full=CLE45p {ECO:0000303|PubMed:16489133, ECO:0000303|PubMed:18034320};
DE Flags: Precursor;
GN Name=CLE45 {ECO:0000303|PubMed:16489133, ECO:0000303|PubMed:18034320};
GN OrderedLocusNames=At1g69588 {ECO:0000312|Araport:AT1G69588};
GN ORFNames=F24J1 {ECO:0000312|EMBL:AC021046};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17229318; DOI=10.1186/1471-2164-8-18;
RA Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.;
RT "Experimental validation of novel genes predicted in the un-annotated
RT regions of the Arabidopsis genome.";
RL BMC Genomics 8:18-18(2007).
RN [6]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [7]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [8]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [9]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH SKM1, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND INDUCTION BY HIGH TEMPERATURE.
RX PubMed=23910659; DOI=10.1016/j.cub.2013.06.060;
RA Endo S., Shinohara H., Matsubayashi Y., Fukuda H.;
RT "A novel pollen-pistil interaction conferring high-temperature tolerance
RT during reproduction via CLE45 signaling.";
RL Curr. Biol. 23:1670-1676(2013).
RN [11]
RP FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=23569225; DOI=10.1073/pnas.1222314110;
RA Depuydt S., Rodriguez-Villalon A., Santuari L., Wyser-Rmili C., Ragni L.,
RA Hardtke C.S.;
RT "Suppression of Arabidopsis protophloem differentiation and root meristem
RT growth by CLE45 requires the receptor-like kinase BAM3.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7074-7079(2013).
RN [12]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=25049386; DOI=10.1073/pnas.1407337111;
RA Rodriguez-Villalon A., Gujas B., Kang Y.H., Breda A.S., Cattaneo P.,
RA Depuydt S., Hardtke C.S.;
RT "Molecular genetic framework for protophloem formation.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11551-11556(2014).
RN [13]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=27354416; DOI=10.15252/embr.201642450;
RA Kang Y.H., Hardtke C.S.;
RT "Arabidopsis MAKR5 is a positive effector of BAM3-dependent CLE45
RT signaling.";
RL EMBO Rep. 17:1145-1154(2016).
RN [14]
RP FUNCTION, AND INTERACTION WITH BAM3.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE45p]: Extracellular signal peptide that regulates cell
CC fate (PubMed:16902140). Represses root apical meristem maintenance
CC (PubMed:16902140, PubMed:23569225). Represses protophloem
CC differentiation in a BAM3-dependent manner (PubMed:23569225). BRX,
CC BAM3, and CLE45 act together to regulate the transition of protophloem
CC cells from proliferation to differentiation, thus impinging on
CC postembryonic growth capacity of the root meristem; this signaling
CC pathway requires CRN and CLV2 and involves MAKR5 for its
CC transduction/amplification (PubMed:23569225, PubMed:27354416,
CC PubMed:28607033). Triggers the accumulation of MAKR5 in developing
CC sieve elements in a BAM3-dependent manner (PubMed:27354416). Prevents,
CC in a dose-dependent manner, auxin response in the root meristem thus
CC leading in the repression of protophloem differentiation and periclinal
CC sieve element precursor cell division (PubMed:25049386). Promotes
CC pollen tube growth prolongation in a SKM1 and SKM2-dependent manner,
CC especially under relatively high temperature (at 30 degrees Celsius),
CC thus conferring tolerance against high temperature probably through the
CC maintenance of mitochondrial activity (PubMed:23910659). Alleviates
CC mitochondrial decay pollen tube in vitro culture (PubMed:23910659).
CC {ECO:0000269|PubMed:16902140, ECO:0000269|PubMed:23569225,
CC ECO:0000269|PubMed:23910659, ECO:0000269|PubMed:25049386,
CC ECO:0000269|PubMed:27354416, ECO:0000269|PubMed:28607033}.
CC -!- SUBUNIT: [CLE45p]: Binds to SKM1 present in the pollen grain,
CC particularly under relatively high temperature (at 30 degrees Celsius)
CC (PubMed:23910659). Interacts with BAM3, especially in roots
CC (PubMed:28607033). {ECO:0000269|PubMed:23910659,
CC ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE45p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE45p]: Expressed at low levels in flowers,
CC especially in pistils (PubMed:16489133, PubMed:23910659). Present in
CC vascular tissues (PubMed:23910659). In roots, confined to protophloem
CC and sieve element precursor cells (PubMed:25049386, PubMed:23569225).
CC {ECO:0000269|PubMed:16489133, ECO:0000269|PubMed:23569225,
CC ECO:0000269|PubMed:23910659, ECO:0000269|PubMed:25049386}.
CC -!- DEVELOPMENTAL STAGE: [CLE45p]: In flowers, at 22 degrees Celsius,
CC preferentially expressed in the stigma in the pistil, but expands to
CC the transmitting tract, along which pollen tubes elongated, upon
CC temperature shift to 30 degrees Celsius (PubMed:23910659). Expressed in
CC roots developing protophloem, up to the end of the transition zone
CC (PubMed:23569225). {ECO:0000269|PubMed:23569225,
CC ECO:0000269|PubMed:23910659}.
CC -!- INDUCTION: [CLE45p]: A shift from 22 degrees Celsius to 30 degrees
CC Celsius leads to a modified spatial repartition in flowers.
CC {ECO:0000269|PubMed:23910659}.
CC -!- DISRUPTION PHENOTYPE: [CLE45p]: Reduced seed production at 30 degrees
CC Celsius, but not at 22 degrees Celsius. {ECO:0000269|PubMed:23910659}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK27998.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY618658; AAT36744.1; -; Genomic_DNA.
DR EMBL; AC021046; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE34953.1; -; Genomic_DNA.
DR EMBL; DQ487459; ABF59305.1; -; Genomic_DNA.
DR EMBL; DQ652620; ABK27998.1; ALT_SEQ; Genomic_DNA.
DR EMBL; EF183256; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001077799.1; NM_001084330.2.
DR AlphaFoldDB; Q6IWA9; -.
DR SMR; Q6IWA9; -.
DR BioGRID; 624052; 1.
DR STRING; 3702.AT1G69588.1; -.
DR PaxDb; Q6IWA9; -.
DR PRIDE; Q6IWA9; -.
DR EnsemblPlants; AT1G69588.1; AT1G69588.1; AT1G69588.
DR GeneID; 5007842; -.
DR Gramene; AT1G69588.1; AT1G69588.1; AT1G69588.
DR KEGG; ath:AT1G69588; -.
DR Araport; AT1G69588; -.
DR TAIR; locus:4010713589; AT1G69588.
DR eggNOG; ENOG502S78B; Eukaryota.
DR HOGENOM; CLU_164336_0_0_1; -.
DR InParanoid; Q6IWA9; -.
DR OMA; DPIHNRT; -.
DR OrthoDB; 1457462at2759; -.
DR PhylomeDB; Q6IWA9; -.
DR PRO; PR:Q6IWA9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; ISS:UniProtKB.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0010928; P:regulation of auxin mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR GO; GO:0080092; P:regulation of pollen tube growth; IDA:UniProtKB.
DR GO; GO:0009266; P:response to temperature stimulus; IMP:UniProtKB.
DR InterPro; IPR038821; CLE45-like.
DR PANTHER; PTHR36726; PTHR36726; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Coiled coil; Developmental protein;
KW Differentiation; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..124
FT /note="CLAVATA3/ESR (CLE)-related protein 45"
FT /id="PRO_5000093477"
FT PEPTIDE 107..118
FT /note="CLE45p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401289"
FT REGION 87..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 71..109
FT /evidence="ECO:0000255"
FT COMPBIAS 87..115
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 124 AA; 14475 MW; 03F419DB2E89825B CRC64;
MLGSSTRSMF FLLVCIGLLA DNRYNVSAMR HREFFLKETQ AEKAGVQTEE ISKLRSIGVQ
FKHTLEDQEM LNKNRRVLEE VNKDKIKAEE TQERKNKTED SFKSSKRRVR RGSDPIHNKA
QPFS
