ID   CLE4D_GLORO             Reviewed;         210 AA.
AC   D1FNK5;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=CLAVATA3/ESR (CLE)-related protein 4D;
DE   Flags: Precursor;
GN   Name=CLE-4D;
OS   Globodera rostochiensis (Golden nematode worm) (Heterodera rostochiensis).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Tylenchina; Tylenchomorpha; Tylenchoidea; Heteroderidae; Heteroderinae;
OC   Globodera.
OX   NCBI_TaxID=31243;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=19656047; DOI=10.1094/mpmi-22-9-1128;
RA   Lu S.-W., Chen S., Wang J., Yu H., Chronis D., Mitchum M.G., Wang X.;
RT   "Structural and functional diversity of CLAVATA3/ESR (CLE)-like genes from
RT   the potato cyst nematode Globodera rostochiensis.";
RL   Mol. Plant Microbe Interact. 22:1128-1142(2009).
CC   -!- FUNCTION: Mimics host plant CLE extracellular signal peptides that
CC       regulate cell fate. May play a role in the differentiation or division
CC       of feeding cells (syncytia) induced in plant roots during infection.
CC       {ECO:0000269|PubMed:19656047}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Host extracellular space {ECO:0000250}. Secreted,
CC       extracellular space, apoplast {ECO:0000250}. Note=Present in secretory
CC       granules within the dorsal esophageal gland secretory cell and in the
CC       dorsal gland ampulla (collecting reservoir) at the base of the nematode
CC       stylet. Secreted into host root cells via the nematode stylet to
CC       transform the recipient cells into enlarged multinucleate feeding cells
CC       called giant-cells or syncytia. Secreted to the host apoplasm from its
CC       cytoplasm via a plant secretory pathway (By similarity). {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Highly expressed exclusively within the dorsal
CC       esophageal gland cell during syncytium formation in host plants.
CC       {ECO:0000269|PubMed:19656047}.
CC   -!- DEVELOPMENTAL STAGE: Strongly up-regulated during root colonization,
CC       from the onset of syncytium formation by parasitic second-stage
CC       juveniles (pJ2) through the J3?J4 molts of sedentary life stages that
CC       become adult females. {ECO:0000269|PubMed:19656047}.
CC   -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC       {ECO:0000305}.
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DR   EMBL; EU386837; ACY70456.1; -; mRNA.
DR   EMBL; EU386844; ACY70463.1; -; Genomic_DNA.
DR   AlphaFoldDB; D1FNK5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043655; C:host extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0033612; F:receptor serine/threonine kinase binding; IEA:InterPro.
DR   GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; IEA:InterPro.
DR   InterPro; IPR044962; CLV3/ESR.
DR   PANTHER; PTHR36349; PTHR36349; 2.
PE   2: Evidence at transcript level;
KW   Apoplast; Differentiation; Glycoprotein; Host cytoplasm; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..210
FT                   /note="CLAVATA3/ESR (CLE)-related protein 4D"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000539265"
FT   REPEAT          127..135
FT                   /note="A-1"
FT   REPEAT          136..147
FT                   /note="CLE-1"
FT   REPEAT          148..156
FT                   /note="A-2"
FT   REPEAT          157..168
FT                   /note="CLE-2"
FT   REPEAT          169..177
FT                   /note="A-3"
FT   REPEAT          178..189
FT                   /note="CLE-3"
FT   REPEAT          190..198
FT                   /note="A-4"
FT   REPEAT          199..210
FT                   /note="CLE-4"
FT   REGION          21..83
FT                   /note="Required for secretion from the host cytoplasm to
FT                   the host apoplasm"
FT                   /evidence="ECO:0000250"
FT   REGION          115..210
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..198
FT                   /note="4 X approximate repeat A"
FT   REGION          136..210
FT                   /note="4 X approximate repeat CLE"
FT   COMPBIAS        115..154
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        169..210
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        32
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        59
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   210 AA;  22979 MW;  D78B68963E22A836 CRC64;
     MAKNAMLCLL ILSVVLALAF ATNKKDDEEP ENHSTGIFGK VGRVVTVALA MSSRLGGANA
     TRGGGAVYGR NLKSNQLPNN NWMAPPPPMA MRSAKVYDSK HSPAEYLKKF AQDFRRKTGT
     HSQRHHEETT LEQEKRGAPA GPDPIHHQDT TFEQEKRGAP AGPDPIHHQD TTLEQEKRVA
     GAGPDPIHHQ DTKFEQEKRG APAGPDPIHH