CLE53_MEDTR
ID CLE53_MEDTR Reviewed; 82 AA.
AC A0A072TRR8;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 1.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 53 {ECO:0000303|PubMed:31477892};
DE Short=MtCLE53 {ECO:0000303|PubMed:31477892};
DE Contains:
DE RecName: Full=CLE53p {ECO:0000305};
DE Flags: Precursor;
GN Name=CLE53 {ECO:0000303|PubMed:31477892};
GN OrderedLocusNames=MTR_8g463700 {ECO:0000312|EMBL:KEH19543.1};
GN ORFNames=MtrunA17_Chr8g0360491 {ECO:0000312|EMBL:RHN40937.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=31477892; DOI=10.1038/s41477-019-0501-1;
RA Mueller L.M., Flokova K., Schnabel E., Sun X., Fei Z., Frugoli J.,
RA Bouwmeester H.J., Harrison M.J.;
RT "A CLE-SUNN module regulates strigolactone content and fungal colonization
RT in arbuscular mycorrhiza.";
RL Nat. Plants 5:933-939(2019).
CC -!- FUNCTION: [CLE53p]: Signaling peptide involved in the regulation of
CC root colonization by arbuscular mycorrhizal (AM) fungi
CC (PubMed:31477892). Moves from root to shoot to function with the
CC receptor kinase SUNN, in a signaling pathway that repress strigolactone
CC biosynthetic genes and strigolactone content in the roots, and
CC consequently reduces the promotion of further colonization by AM fungi
CC (PubMed:31477892). {ECO:0000269|PubMed:31477892}.
CC -!- SUBCELLULAR LOCATION: [CLE53p]: Secreted, extracellular space
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: [CLE53p]: Expressed in root vasculature.
CC {ECO:0000269|PubMed:31477892}.
CC -!- INDUCTION: [CLE53p]: Induced in vasculature of roots colonized with the
CC arbuscular mycorrhizal (AM) fungi Rhizophagus irregularis and Glomus
CC versiforme. {ECO:0000269|PubMed:31477892}.
CC -!- PTM: [CLE53p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-77 enhances binding affinity of the CLE53p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- MISCELLANEOUS: Roots of plants overexpressing CLE53 exhibit normal
CC growth, but reduced levels of colonization by arbuscular mycorrhizal
CC (AM) fungi (PubMed:31477892). Roots of plants silencing CLE53 exhibit
CC normal growth, but reduced levels of colonization by arbuscular
CC mycorrhizal (AM) fungi (PubMed:31477892).
CC {ECO:0000269|PubMed:31477892}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
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DR EMBL; CM001224; KEH19543.1; -; Genomic_DNA.
DR EMBL; PSQE01000008; RHN40937.1; -; Genomic_DNA.
DR RefSeq; XP_013445517.1; XM_013590063.1.
DR AlphaFoldDB; A0A072TRR8; -.
DR EnsemblPlants; KEH19543; KEH19543; MTR_8g463700.
DR Gramene; KEH19543; KEH19543; MTR_8g463700.
DR KEGG; mtr:MTR_8g463700; -.
DR HOGENOM; CLU_154904_2_0_1; -.
DR OrthoDB; 1642840at2759; -.
DR Proteomes; UP000002051; Chromosome 8.
DR Proteomes; UP000265566; Chromosome 8.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; IEA:InterPro.
DR InterPro; IPR039617; CLAVATA3-CLE.
DR PANTHER; PTHR36016; PTHR36016; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..82
FT /note="CLAVATA3/ESR (CLE)-related protein 53"
FT /id="PRO_5014498923"
FT PEPTIDE 71..82
FT /note="CLE53p"
FT /id="PRO_0000448637"
FT MOD_RES 74
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MOD_RES 77
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 77
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
SQ SEQUENCE 82 AA; 9280 MW; 2CC3AA7045B981AA CRC64;
MATSTNSREF LIFICVLTLL VVRSEARLVP MLFSTKEKVM NSKFGLREVI NDVRNSEWLR
KRALLGGKPE RVSPGGPDAQ HH
