CLE8_ARATH
ID CLE8_ARATH Reviewed; 86 AA.
AC Q2V4E2; A0MDK3; Q1G3X6;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=CLAVATA3/ESR (CLE)-related protein 8 {ECO:0000303|PubMed:11457943};
DE Contains:
DE RecName: Full=CLE8p {ECO:0000303|PubMed:11457943};
DE Flags: Precursor;
GN Name=CLE8 {ECO:0000303|PubMed:11457943};
GN OrderedLocusNames=At1g67775 {ECO:0000312|Araport:AT1G67775};
GN ORFNames=F12A21 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 23-86.
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11457943; DOI=10.1104/pp.126.3.939;
RA Cock J.M., McCormick S.;
RT "A large family of genes that share homology with CLAVATA3.";
RL Plant Physiol. 126:939-942(2001).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12602871; DOI=10.1023/a:1022038932376;
RA Sharma V.K., Ramirez J., Fletcher J.C.;
RT "The Arabidopsis CLV3-like (CLE) genes are expressed in diverse tissues and
RT encode secreted proteins.";
RL Plant Mol. Biol. 51:415-425(2003).
RN [6]
RP FUNCTION.
RX PubMed=16407446; DOI=10.1104/pp.105.072678;
RA Ni J., Clark S.E.;
RT "Evidence for functional conservation, sufficiency, and proteolytic
RT processing of the CLAVATA3 CLE domain.";
RL Plant Physiol. 140:726-733(2006).
RN [7]
RP GENE FAMILY.
RX PubMed=16489133; DOI=10.1104/pp.105.075515;
RA Strabala T.J., O'donnell P.J., Smit A.-M., Ampomah-Dwamena C., Martin E.J.,
RA Netzler N., Nieuwenhuizen N.J., Quinn B.D., Foote H.C.C., Hudson K.R.;
RT "Gain-of-function phenotypes of many CLAVATA3/ESR genes, including four new
RT family members, correlate with tandem variations in the conserved
RT CLAVATA3/ESR domain.";
RL Plant Physiol. 140:1331-1344(2006).
RN [8]
RP FUNCTION.
RX PubMed=16902140; DOI=10.1126/science.1128436;
RA Ito Y., Nakanomyo I., Motose H., Iwamoto K., Sawa S., Dohmae N., Fukuda H.;
RT "Dodeca-CLE peptides as suppressors of plant stem cell differentiation.";
RL Science 313:842-845(2006).
RN [9]
RP REVIEW.
RX PubMed=18034320; DOI=10.1007/s00018-007-7411-5;
RA Jun J.H., Fiume E., Fletcher J.C.;
RT "The CLE family of plant polypeptide signaling molecules.";
RL Cell. Mol. Life Sci. 65:743-755(2008).
RN [10]
RP REVIEW.
RX PubMed=18078779; DOI=10.1016/j.pbi.2007.10.010;
RA Mitchum M.G., Wang X., Davis E.L.;
RT "Diverse and conserved roles of CLE peptides.";
RL Curr. Opin. Plant Biol. 11:75-81(2008).
RN [11]
RP REVIEW.
RX PubMed=20016993; DOI=10.1007/s00709-009-0095-y;
RA Wang G., Fiers M.;
RT "CLE peptide signaling during plant development.";
RL Protoplasma 240:33-43(2010).
RN [12]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF PRO-67.
RX PubMed=22427333; DOI=10.1105/tpc.111.094839;
RA Fiume E., Fletcher J.C.;
RT "Regulation of Arabidopsis embryo and endosperm development by the
RT polypeptide signaling molecule CLE8.";
RL Plant Cell 24:1000-1012(2012).
RN [13]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=28607033; DOI=10.15252/embr.201643535;
RA Hazak O., Brandt B., Cattaneo P., Santiago J., Rodriguez-Villalon A.,
RA Hothorn M., Hardtke C.S.;
RT "Perception of root-active CLE peptides requires CORYNE function in the
RT phloem vasculature.";
RL EMBO Rep. 18:1367-1381(2017).
CC -!- FUNCTION: [CLE8p]: Extracellular signal peptide that regulates cell
CC fate (PubMed:16407446). Represses root apical meristem maintenance
CC (PubMed:16902140). Positively regulates the expression of the
CC transcription factor WOX8 and thus, regulates early embryo development
CC (PubMed:22427333). Regulates the transition of protophloem cells from
CC proliferation to differentiation, thus impinging on postembryonic
CC growth capacity of the root meristem; this signaling pathway requires
CC CRN and CLV2 (PubMed:28607033). {ECO:0000269|PubMed:16407446,
CC ECO:0000269|PubMed:16902140, ECO:0000269|PubMed:22427333,
CC ECO:0000269|PubMed:28607033}.
CC -!- SUBCELLULAR LOCATION: [CLE8p]: Secreted, extracellular space
CC {ECO:0000250|UniProtKB:O49519}.
CC -!- TISSUE SPECIFICITY: [CLE8p]: Mostly expressed in siliques, and, to a
CC lower extent, in flowers (PubMed:12602871). Expressed in young embryos
CC and endosperm (PubMed:22427333). {ECO:0000269|PubMed:12602871,
CC ECO:0000269|PubMed:22427333}.
CC -!- PTM: [CLE8p]: The O-glycosylation (arabinosylation) of the
CC hydroxyproline Pro-67 enhances binding affinity of the CLE8p peptide
CC for its receptor. {ECO:0000250|UniProtKB:O49519}.
CC -!- SIMILARITY: Belongs to the CLV3/ESR signal peptide family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28316.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC008113; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE34693.1; -; Genomic_DNA.
DR EMBL; DQ487458; ABF59187.1; -; mRNA.
DR EMBL; DQ652619; ABK28316.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001031249.1; NM_001036172.2.
DR AlphaFoldDB; Q2V4E2; -.
DR STRING; 3702.AT1G67775.1; -.
DR PaxDb; Q2V4E2; -.
DR PRIDE; Q2V4E2; -.
DR EnsemblPlants; AT1G67775.1; AT1G67775.1; AT1G67775.
DR GeneID; 3767662; -.
DR Gramene; AT1G67775.1; AT1G67775.1; AT1G67775.
DR KEGG; ath:AT1G67775; -.
DR Araport; AT1G67775; -.
DR TAIR; locus:1009023120; AT1G67775.
DR HOGENOM; CLU_192459_0_0_1; -.
DR OMA; LPLFRIM; -.
DR OrthoDB; 1637982at2759; -.
DR PhylomeDB; Q2V4E2; -.
DR PRO; PR:Q2V4E2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q2V4E2; baseline and differential.
DR GO; GO:0048046; C:apoplast; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0033612; F:receptor serine/threonine kinase binding; ISS:UniProtKB.
DR GO; GO:0045168; P:cell-cell signaling involved in cell fate commitment; ISS:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0010078; P:maintenance of root meristem identity; IDA:UniProtKB.
DR GO; GO:0010088; P:phloem development; IDA:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:TAIR.
DR GO; GO:0045595; P:regulation of cell differentiation; IDA:UniProtKB.
DR GO; GO:2000014; P:regulation of endosperm development; IMP:TAIR.
DR GO; GO:0080113; P:regulation of seed growth; IMP:TAIR.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Glycoprotein; Hydroxylation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..86
FT /note="CLAVATA3/ESR (CLE)-related protein 8"
FT /id="PRO_0000401247"
FT PEPTIDE 61..72
FT /note="CLE8p"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT /id="PRO_0000401248"
FT REGION 43..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 64
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:A1EC31"
FT MOD_RES 67
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:A1EC31"
FT CARBOHYD 67
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT MUTAGEN 67
FT /note="P->L: In cle8-1; defective embryo development."
FT /evidence="ECO:0000269|PubMed:22427333"
SQ SEQUENCE 86 AA; 9703 MW; E79919E910D81C9C CRC64;
MKVLKRDSML LLITLYFLLT TSMARQDPFL VGVEKDVVPA GTDLKQNKAK PHLPNLFRTM
RRVPTGPNPL HHISPPQPGS LNYARN
