CLF1_SCHPO
ID CLF1_SCHPO Reviewed; 674 AA.
AC P87312;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Pre-mRNA-splicing factor cwf4;
DE AltName: Full=Complexed with cdc5 protein 4;
GN Name=cwf4; Synonyms=syf3; ORFNames=SPBC31F10.11c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 12-25; 40-54 AND
RP 225-242.
RX PubMed=10409726; DOI=10.1128/mcb.19.8.5352;
RA McDonald W.H., Ohi R., Smelkova N., Frendewey D., Gould K.L.;
RT "Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing
RT complex and is essential for pre-mRNA splicing.";
RL Mol. Cell. Biol. 19:5352-5362(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
CC -!- FUNCTION: Involved in pre-mRNA splicing and cell cycle progression.
CC Required for the spliceosome assembly and initiation of the DNA
CC replication (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC -!- INTERACTION:
CC P87312; P39964: cdc5; NbExp=7; IntAct=EBI-538818, EBI-538771;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the crooked-neck family. {ECO:0000305}.
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DR EMBL; AF254353; AAF67752.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB10088.1; -; Genomic_DNA.
DR PIR; T40214; T40214.
DR RefSeq; NP_596573.1; NM_001022494.2.
DR PDB; 3JB9; EM; 3.60 A; R=41-290.
DR PDBsum; 3JB9; -.
DR AlphaFoldDB; P87312; -.
DR SMR; P87312; -.
DR BioGRID; 276858; 30.
DR IntAct; P87312; 8.
DR STRING; 4896.SPBC31F10.11c.1; -.
DR MaxQB; P87312; -.
DR PaxDb; P87312; -.
DR PRIDE; P87312; -.
DR EnsemblFungi; SPBC31F10.11c.1; SPBC31F10.11c.1:pep; SPBC31F10.11c.
DR GeneID; 2540328; -.
DR KEGG; spo:SPBC31F10.11c; -.
DR PomBase; SPBC31F10.11c; cwf4.
DR VEuPathDB; FungiDB:SPBC31F10.11c; -.
DR eggNOG; KOG1915; Eukaryota.
DR HOGENOM; CLU_011554_1_0_1; -.
DR InParanoid; P87312; -.
DR OMA; HIKVWIS; -.
DR PhylomeDB; P87312; -.
DR PRO; PR:P87312; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR GO; GO:0000974; C:Prp19 complex; IDA:PomBase.
DR GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; ISO:PomBase.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IC:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000245; P:spliceosomal complex assembly; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR045075; Syf1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR11246; PTHR11246; 1.
DR Pfam; PF02184; HAT; 2.
DR SMART; SM00386; HAT; 14.
DR SUPFAM; SSF48452; SSF48452; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Repeat; Spliceosome.
FT CHAIN 1..674
FT /note="Pre-mRNA-splicing factor cwf4"
FT /id="PRO_0000205750"
FT REPEAT 50..82
FT /note="HAT 1"
FT REPEAT 84..116
FT /note="HAT 2"
FT REPEAT 118..150
FT /note="HAT 3"
FT REPEAT 152..183
FT /note="HAT 4"
FT REPEAT 185..216
FT /note="HAT 5"
FT REPEAT 218..253
FT /note="HAT 6"
FT REPEAT 255..289
FT /note="HAT 7"
FT REPEAT 299..331
FT /note="HAT 8"
FT REPEAT 333..367
FT /note="HAT 9"
FT REPEAT 377..413
FT /note="HAT 10"
FT REPEAT 415..446
FT /note="HAT 11"
FT REPEAT 448..480
FT /note="HAT 12"
FT REPEAT 482..516
FT /note="HAT 13"
FT REPEAT 518..549
FT /note="HAT 14"
FT REPEAT 567..608
FT /note="HAT 15"
FT REPEAT 610..643
FT /note="HAT 16"
SQ SEQUENCE 674 AA; 80841 MW; 1AFAA23A68CB2A9F CRC64;
MTSEAPRVKN KNPAPIQISA EQLLREAVER QDVAFVPPKI NITDLEELQE FQGRKRKEFE
DAIRRNRLAM GHWMRYGQWE LDQKEFARAR SVFERALDVD STYIPLWLKY IECEMKNRNI
NHARNLFDRA VTQLPRVDKL WYKYVYMEEM LGNITGCRQV FERWLKWEPD ENCWMSYIRM
ERRYHENERA RGIYERFVVV HPEVTNWLRW ARFEEECGNA ANVRQVYLAA IDALGQEFLN
ERFFIAFAKF EIRQKEYERA RTIFKYAIDF MPRSKSMELY KEYTHFEKQF GDHLGVESTV
LDKRRLQYEK LLKDSPYDYD TWLDLLKLEE SAGDINTIRE TYEKAIAKVP EVVEKNAWRR
YVYIWLNYCL FEEIDVKDVD RARKVYQEAL KLIPHKKFTF AKLWLMYAMF ELRQRKIDVA
RKTLGRALGM CPKPKLFRGY IEFEDAIKQF DRCRILYEKW ILYDPEACAP WLGYAALETK
LGDSDRARAL YNLAVNQPIL ETPELVWKAY IDFEFEEMEY GKARSIYQQL LRTAPHVKVW
ISFANFEIAH LEDDDEEPPN EEVASPTAVV RARNVFENAL AHLRQQGLKE ERVVLLEAWK
QFEAMHGTED TRKHVSSLMP QVVKKRRRLE DGSFEEYLDY LFPDTATDQG DKMRKMLELS
RKWKEEMAKK KLEA
