CLGR_MYCS2
ID CLGR_MYCS2 Reviewed; 112 AA.
AC A0QVU1;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Transcriptional regulator ClgR {ECO:0000305};
GN Name=clgR; OrderedLocusNames=MSMEG_2694, MSMEI_2628;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=20025669; DOI=10.1111/j.1365-2958.2009.07008.x;
RA Barik S., Sureka K., Mukherjee P., Basu J., Kundu M.;
RT "RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis,
RT is inactivated by phosphorylation-dependent ClpC1P2 proteolysis.";
RL Mol. Microbiol. 75:592-606(2010).
RN [5] {ECO:0007744|PDB:5WOQ}
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RA Edwards T.E., Mayclin S.J., Lorimer D.D.;
RT "Crystal structure of an XRE family protein transcriptional regulator from
RT Mycobacterium smegmatis.";
RL Submitted (AUG-2017) to the PDB data bank.
CC -!- FUNCTION: Key stress-response regulator that plays an important role in
CC multiple regulatory networks in response to different stress conditions
CC (By similarity). Probably controls the response to vancomycin
CC treatment; vancomycin induces expression of the clp regulon (clpP1,
CC clpP2 and clpC1) (PubMed:20025669). {ECO:0000250|UniProtKB:P9WMH7,
CC ECO:0000269|PubMed:20025669}.
CC -!- INDUCTION: By vancomycin, requires sigE. {ECO:0000269|PubMed:20025669}.
CC -!- DISRUPTION PHENOTYPE: Depletion experiments (via anti-sense RNA) show
CC greatly reduced induction of the clp regulon (clpP1, clpP2 and clpC1)
CC after vancomycin treatment. {ECO:0000269|PubMed:20025669}.
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DR EMBL; CP000480; ABK69606.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP39096.1; -; Genomic_DNA.
DR RefSeq; WP_003894079.1; NZ_SIJM01000059.1.
DR RefSeq; YP_887029.1; NC_008596.1.
DR PDB; 5WOQ; X-ray; 1.95 A; A/B/C=1-112.
DR PDBsum; 5WOQ; -.
DR AlphaFoldDB; A0QVU1; -.
DR SMR; A0QVU1; -.
DR STRING; 246196.MSMEI_2628; -.
DR PRIDE; A0QVU1; -.
DR EnsemblBacteria; ABK69606; ABK69606; MSMEG_2694.
DR EnsemblBacteria; AFP39096; AFP39096; MSMEI_2628.
DR GeneID; 66734101; -.
DR KEGG; msg:MSMEI_2628; -.
DR KEGG; msm:MSMEG_2694; -.
DR PATRIC; fig|246196.19.peg.2661; -.
DR eggNOG; COG1396; Bacteria.
DR OMA; PAPMRPM; -.
DR OrthoDB; 2063007at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..112
FT /note="Transcriptional regulator ClgR"
FT /id="PRO_0000423654"
FT DOMAIN 13..67
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 24..43
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT HELIX 5..18
FT /evidence="ECO:0007829|PDB:5WOQ"
FT TURN 19..21
FT /evidence="ECO:0007829|PDB:5WOQ"
FT HELIX 24..31
FT /evidence="ECO:0007829|PDB:5WOQ"
FT HELIX 35..42
FT /evidence="ECO:0007829|PDB:5WOQ"
FT HELIX 50..60
FT /evidence="ECO:0007829|PDB:5WOQ"
FT HELIX 64..80
FT /evidence="ECO:0007829|PDB:5WOQ"
SQ SEQUENCE 112 AA; 12060 MW; EE2AD0698D80E4FD CRC64;
MTALLREVIG DVLRNARTDQ GRTLREVSDA ARVSLGYLSE VERGRKEASS ELLSAICDAL
DVPLSRVLTD AGESMARREH DAREAEQVAV ANLGRIDAAT KVVIPQVSMA VA