ID   CLGR_MYCS2              Reviewed;         112 AA.
AC   A0QVU1;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Transcriptional regulator ClgR {ECO:0000305};
GN   Name=clgR; OrderedLocusNames=MSMEG_2694, MSMEI_2628;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=20025669; DOI=10.1111/j.1365-2958.2009.07008.x;
RA   Barik S., Sureka K., Mukherjee P., Basu J., Kundu M.;
RT   "RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis,
RT   is inactivated by phosphorylation-dependent ClpC1P2 proteolysis.";
RL   Mol. Microbiol. 75:592-606(2010).
RN   [5] {ECO:0007744|PDB:5WOQ}
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RA   Edwards T.E., Mayclin S.J., Lorimer D.D.;
RT   "Crystal structure of an XRE family protein transcriptional regulator from
RT   Mycobacterium smegmatis.";
RL   Submitted (AUG-2017) to the PDB data bank.
CC   -!- FUNCTION: Key stress-response regulator that plays an important role in
CC       multiple regulatory networks in response to different stress conditions
CC       (By similarity). Probably controls the response to vancomycin
CC       treatment; vancomycin induces expression of the clp regulon (clpP1,
CC       clpP2 and clpC1) (PubMed:20025669). {ECO:0000250|UniProtKB:P9WMH7,
CC       ECO:0000269|PubMed:20025669}.
CC   -!- INDUCTION: By vancomycin, requires sigE. {ECO:0000269|PubMed:20025669}.
CC   -!- DISRUPTION PHENOTYPE: Depletion experiments (via anti-sense RNA) show
CC       greatly reduced induction of the clp regulon (clpP1, clpP2 and clpC1)
CC       after vancomycin treatment. {ECO:0000269|PubMed:20025669}.
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DR   EMBL; CP000480; ABK69606.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP39096.1; -; Genomic_DNA.
DR   RefSeq; WP_003894079.1; NZ_SIJM01000059.1.
DR   RefSeq; YP_887029.1; NC_008596.1.
DR   PDB; 5WOQ; X-ray; 1.95 A; A/B/C=1-112.
DR   PDBsum; 5WOQ; -.
DR   AlphaFoldDB; A0QVU1; -.
DR   SMR; A0QVU1; -.
DR   STRING; 246196.MSMEI_2628; -.
DR   PRIDE; A0QVU1; -.
DR   EnsemblBacteria; ABK69606; ABK69606; MSMEG_2694.
DR   EnsemblBacteria; AFP39096; AFP39096; MSMEI_2628.
DR   GeneID; 66734101; -.
DR   KEGG; msg:MSMEI_2628; -.
DR   KEGG; msm:MSMEG_2694; -.
DR   PATRIC; fig|246196.19.peg.2661; -.
DR   eggNOG; COG1396; Bacteria.
DR   OMA; PAPMRPM; -.
DR   OrthoDB; 2063007at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   SMART; SM00530; HTH_XRE; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS50943; HTH_CROC1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..112
FT                   /note="Transcriptional regulator ClgR"
FT                   /id="PRO_0000423654"
FT   DOMAIN          13..67
FT                   /note="HTH cro/C1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   DNA_BIND        24..43
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   HELIX           5..18
FT                   /evidence="ECO:0007829|PDB:5WOQ"
FT   TURN            19..21
FT                   /evidence="ECO:0007829|PDB:5WOQ"
FT   HELIX           24..31
FT                   /evidence="ECO:0007829|PDB:5WOQ"
FT   HELIX           35..42
FT                   /evidence="ECO:0007829|PDB:5WOQ"
FT   HELIX           50..60
FT                   /evidence="ECO:0007829|PDB:5WOQ"
FT   HELIX           64..80
FT                   /evidence="ECO:0007829|PDB:5WOQ"
SQ   SEQUENCE   112 AA;  12060 MW;  EE2AD0698D80E4FD CRC64;
     MTALLREVIG DVLRNARTDQ GRTLREVSDA ARVSLGYLSE VERGRKEASS ELLSAICDAL
     DVPLSRVLTD AGESMARREH DAREAEQVAV ANLGRIDAAT KVVIPQVSMA VA