CLH1_CHEAL
ID CLH1_CHEAL Reviewed; 22 AA.
AC P59677;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2003, sequence version 1.
DT 11-DEC-2019, entry version 35.
DE RecName: Full=Chlorophyllase type 1;
DE EC=3.1.1.14;
DE AltName: Full=CaCLH1;
DE AltName: Full=Chlorophyll-chlorophyllido hydrolase 1;
DE Short=Chlase 1;
DE Flags: Fragments;
OS Chenopodium album (Fat-hen).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Atripliceae; Chenopodium.
OX NCBI_TaxID=3559;
RN [1]
RP PROTEIN SEQUENCE, AND CHARACTERIZATION.
RA Tsuchiya T., Ohta H., Masuda T., Mikami B., Kita N., Shioi Y., Takamiya K.;
RT "Purification and characterization of two isozymes of chlorophyllase from
RT mature leaves of Chenopodium album.";
RL Plant Cell Physiol. 38:1026-1031(1997).
CC -!- FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to
CC yield chlorophyllide and phytol.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol;
CC Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292;
CC EC=3.1.1.14;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=4.0 uM for chlorophyll a;
CC KM=3.1 uM for chlorophyll b;
CC pH dependence:
CC Optimum pH is 6.6-8.6 for chlorophyll a hydrolysis, and 6.6-7.6 for
CC chlorophyll b hydrolysis.;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC degradation.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
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DR UniPathway; UPA00674; -.
DR GO; GO:0047746; F:chlorophyllase activity; IEA:UniProtKB-EC.
DR GO; GO:0102293; F:pheophytinase b activity; IEA:UniProtKB-EC.
DR GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-UniPathway.
PE 1: Evidence at protein level;
KW Chlorophyll catabolism; Direct protein sequencing; Hydrolase.
FT CHAIN 1..>22
FT /note="Chlorophyllase type 1"
FT /id="PRO_0000090387"
FT UNSURE 17
FT NON_CONS 15..16
FT /evidence="ECO:0000305"
FT NON_TER 22
SQ SEQUENCE 22 AA; 2524 MW; 4CFF9D11EE7ABE92 CRC64;
SEKITVFQKG NFQVTEXSSH FV