CLH1_CITUN
ID CLH1_CITUN Reviewed; 329 AA.
AC Q94LX1;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Chlorophyllase-1, chloroplastic;
DE EC=3.1.1.14;
DE AltName: Full=Chlorophyll-chlorophyllido hydrolase 1;
DE Short=Chlase 1;
DE Flags: Precursor;
OS Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=55188;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nichinan No. 1;
RA Azuma R.K., Kurata H., Shimokawa K., Adachi M.;
RT "Chlorophyllase on ethylene-treated Citrus unshiu Marc.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to
CC yield chlorophyllide and phytol.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol;
CC Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292;
CC EC=3.1.1.14;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC degradation.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB056126; BAB47176.1; -; mRNA.
DR AlphaFoldDB; Q94LX1; -.
DR SMR; Q94LX1; -.
DR ESTHER; citsi-Q9MV14; Chlorophyllase.
DR BRENDA; 3.1.1.14; 1428.
DR UniPathway; UPA00674; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0047746; F:chlorophyllase activity; IEA:UniProtKB-EC.
DR GO; GO:0102293; F:pheophytinase b activity; IEA:UniProtKB-EC.
DR GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR017395; Chlorophyllase.
DR Pfam; PF07224; Chlorophyllase; 1.
DR PIRSF; PIRSF038128; Chlorophyllase_chloroplast; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00120; LIPASE_SER; 1.
PE 2: Evidence at transcript level;
KW Chlorophyll catabolism; Chloroplast; Hydrolase; Plastid; Transit peptide.
FT TRANSIT 1..21
FT /note="Chloroplast"
FT /evidence="ECO:0000250"
FT CHAIN 22..329
FT /note="Chlorophyllase-1, chloroplastic"
FT /id="PRO_0000017840"
FT MOTIF 145..149
FT /note="GXSXG"
FT /evidence="ECO:0000250|UniProtKB:Q948R1"
FT ACT_SITE 147
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9LE89"
FT ACT_SITE 169
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q9LE89"
FT ACT_SITE 242
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q9LE89"
SQ SEQUENCE 329 AA; 35249 MW; 3FA347B74F88C21D CRC64;
MAAMVDSKPA ASVQGTPLLA TATLPVFTRG IYSTKRITLE TSSPSSPPPP KPLIIVTPAE
KGTFNVILFL HGTSLSNKSY SKIFDHIASH GFIVVAPQLY TSIPPPSATN ELNSAAEVAE
WLPQGLQQNL PENTEANVSL VAVMGHSRGG QTAFALSLRY GFGAVIGLDP VAGTSKTTGL
DPSILSFDSF DFSIPVTVIG TGLGGVARCI TACAPEGANH EEFFNRCKNS SRAHFVATDY
GHMDILDDNP SDVKSWALSK YFCKNGNESR DPMRRCVSGI VVAFLKDFFD GDAEDFRQIL
KDPSFAPIKL DSVEYIDASS MLTTTHVKV