ID   CLH1_CITUN              Reviewed;         329 AA.
AC   Q94LX1;
DT   16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Chlorophyllase-1, chloroplastic;
DE            EC=3.1.1.14;
DE   AltName: Full=Chlorophyll-chlorophyllido hydrolase 1;
DE            Short=Chlase 1;
DE   Flags: Precursor;
OS   Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=55188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nichinan No. 1;
RA   Azuma R.K., Kurata H., Shimokawa K., Adachi M.;
RT   "Chlorophyllase on ethylene-treated Citrus unshiu Marc.";
RL   Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to
CC       yield chlorophyllide and phytol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol;
CC         Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292;
CC         EC=3.1.1.14;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       degradation.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC       {ECO:0000305}.
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DR   EMBL; AB056126; BAB47176.1; -; mRNA.
DR   AlphaFoldDB; Q94LX1; -.
DR   SMR; Q94LX1; -.
DR   ESTHER; citsi-Q9MV14; Chlorophyllase.
DR   BRENDA; 3.1.1.14; 1428.
DR   UniPathway; UPA00674; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0047746; F:chlorophyllase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102293; F:pheophytinase b activity; IEA:UniProtKB-EC.
DR   GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR017395; Chlorophyllase.
DR   Pfam; PF07224; Chlorophyllase; 1.
DR   PIRSF; PIRSF038128; Chlorophyllase_chloroplast; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00120; LIPASE_SER; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll catabolism; Chloroplast; Hydrolase; Plastid; Transit peptide.
FT   TRANSIT         1..21
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000250"
FT   CHAIN           22..329
FT                   /note="Chlorophyllase-1, chloroplastic"
FT                   /id="PRO_0000017840"
FT   MOTIF           145..149
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000250|UniProtKB:Q948R1"
FT   ACT_SITE        147
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LE89"
FT   ACT_SITE        169
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LE89"
FT   ACT_SITE        242
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LE89"
SQ   SEQUENCE   329 AA;  35249 MW;  3FA347B74F88C21D CRC64;
     MAAMVDSKPA ASVQGTPLLA TATLPVFTRG IYSTKRITLE TSSPSSPPPP KPLIIVTPAE
     KGTFNVILFL HGTSLSNKSY SKIFDHIASH GFIVVAPQLY TSIPPPSATN ELNSAAEVAE
     WLPQGLQQNL PENTEANVSL VAVMGHSRGG QTAFALSLRY GFGAVIGLDP VAGTSKTTGL
     DPSILSFDSF DFSIPVTVIG TGLGGVARCI TACAPEGANH EEFFNRCKNS SRAHFVATDY
     GHMDILDDNP SDVKSWALSK YFCKNGNESR DPMRRCVSGI VVAFLKDFFD GDAEDFRQIL
     KDPSFAPIKL DSVEYIDASS MLTTTHVKV