ID   CLH2_CHEAL              Reviewed;          21 AA.
AC   P59678;
DT   16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2003, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Chlorophyllase type 2;
DE            EC=3.1.1.14;
DE   AltName: Full=CaCLH2;
DE   AltName: Full=Chlorophyll-chlorophyllido hydrolase 2;
DE            Short=Chlase 2;
DE   Flags: Fragments;
OS   Chenopodium album (Fat-hen).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Atripliceae; Chenopodium.
OX   NCBI_TaxID=3559;
RN   [1]
RP   PROTEIN SEQUENCE, AND CHARACTERIZATION.
RA   Tsuchiya T., Ohta H., Masuda T., Mikami B., Kita N., Shioi Y., Takamiya K.;
RT   "Purification and characterization of two isozymes of chlorophyllase from
RT   mature leaves of Chenopodium album.";
RL   Plant Cell Physiol. 38:1026-1031(1997).
CC   -!- FUNCTION: Catalyzes the hydrolysis of ester bond in chlorophyll to
CC       yield chlorophyllide and phytol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chlorophyll + H2O = a chlorophyllide + H(+) + phytol;
CC         Xref=Rhea:RHEA:19605, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17327, ChEBI:CHEBI:139291, ChEBI:CHEBI:139292;
CC         EC=3.1.1.14;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=4.6 uM for chlorophyll a;
CC         KM=4.4 uM for chlorophyll b;
CC       pH dependence:
CC         Optimum pH is 6.6-8.6 for chlorophyll a hydrolysis, and 6.6-7.6 for
CC         chlorophyll b hydrolysis.;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       degradation.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P59678; -.
DR   UniPathway; UPA00674; -.
DR   GO; GO:0047746; F:chlorophyllase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102293; F:pheophytinase b activity; IEA:UniProtKB-EC.
DR   GO; GO:0015996; P:chlorophyll catabolic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   Chlorophyll catabolism; Direct protein sequencing; Hydrolase.
FT   CHAIN           1..>21
FT                   /note="Chlorophyllase type 2"
FT                   /id="PRO_0000090388"
FT   NON_CONS        14..15
FT                   /evidence="ECO:0000305"
FT   NON_TER         21
SQ   SEQUENCE   21 AA;  2470 MW;  385CA49D18E1D89D CRC64;
     SEKITVFQKN FQVTENSSHF V