CLIC2_RAT
ID CLIC2_RAT Reviewed; 245 AA.
AC Q5M883;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Chloride intracellular channel protein 2;
GN Name=Clic2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Can insert into membranes and form chloride ion channels.
CC Channel activity depends on the pH. Membrane insertion seems to be
CC redox-regulated and may occur only under oxydizing conditions.
CC Modulates the activity of RYR2 and inhibits calcium influx (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Monomer. Interacts with TRAPPC2 and RYR2 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}. Note=Exists both as soluble
CC cytoplasmic protein and as membrane protein with probably a single
CC transmembrane domain. {ECO:0000250}.
CC -!- DOMAIN: Members of this family may change from a globular, soluble
CC state to a state where the N-terminal domain is inserted into the
CC membrane and functions as chloride channel. A conformation change of
CC the N-terminal domain is thought to expose hydrophobic surfaces that
CC trigger membrane insertion (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the chloride channel CLIC family. {ECO:0000305}.
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DR EMBL; BC088182; AAH88182.1; -; mRNA.
DR RefSeq; NP_001009651.1; NM_001009651.1.
DR AlphaFoldDB; Q5M883; -.
DR SMR; Q5M883; -.
DR STRING; 10116.ENSRNOP00000000955; -.
DR iPTMnet; Q5M883; -.
DR PhosphoSitePlus; Q5M883; -.
DR PaxDb; Q5M883; -.
DR PRIDE; Q5M883; -.
DR Ensembl; ENSRNOT00000000955; ENSRNOP00000000955; ENSRNOG00000000728.
DR GeneID; 294141; -.
DR KEGG; rno:294141; -.
DR UCSC; RGD:1306580; rat.
DR CTD; 1193; -.
DR RGD; 1306580; Clic2.
DR eggNOG; KOG1422; Eukaryota.
DR GeneTree; ENSGT00940000161397; -.
DR HOGENOM; CLU_061051_1_0_1; -.
DR InParanoid; Q5M883; -.
DR OMA; IHTCPED; -.
DR OrthoDB; 974249at2759; -.
DR PhylomeDB; Q5M883; -.
DR TreeFam; TF315438; -.
DR Reactome; R-RNO-2672351; Stimuli-sensing channels.
DR Reactome; R-RNO-5578775; Ion homeostasis.
DR PRO; PR:Q5M883; -.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000000728; Expressed in spleen and 18 other tissues.
DR Genevisible; Q5M883; RN.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005254; F:chloride channel activity; IBA:GO_Central.
DR GO; GO:0004602; F:glutathione peroxidase activity; ISO:RGD.
DR GO; GO:0005244; F:voltage-gated ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0006821; P:chloride transport; IBA:GO_Central.
DR GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR GO; GO:0060315; P:negative regulation of ryanodine-sensitive calcium-release channel activity; ISO:RGD.
DR GO; GO:0051099; P:positive regulation of binding; ISO:RGD.
DR GO; GO:0010880; P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; ISO:RGD.
DR InterPro; IPR002946; CLIC.
DR InterPro; IPR030253; CLIC-2.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR InterPro; IPR040079; Glutathione_S-Trfase.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR43920:SF4; PTHR43920:SF4; 1.
DR Pfam; PF13409; GST_N_2; 1.
DR PRINTS; PR01263; INTCLCHANNEL.
DR SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR TIGRFAMs; TIGR00862; O-ClC; 1.
DR PROSITE; PS50405; GST_CTER; 1.
PE 2: Evidence at transcript level;
KW Chloride; Chloride channel; Cytoplasm; Disulfide bond; Ion channel;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..245
FT /note="Chloride intracellular channel protein 2"
FT /id="PRO_0000144206"
FT TRANSMEM 32..52
FT /note="Helical; Note=After insertion into the membrane"
FT /evidence="ECO:0000255"
FT DOMAIN 99..239
FT /note="GST C-terminal"
FT REGION 1..96
FT /note="Required for insertion into the membrane"
FT /evidence="ECO:0000250"
FT DISULFID 30..33
FT /note="In soluble form"
FT /evidence="ECO:0000250"
SQ SEQUENCE 245 AA; 28161 MW; DE6379E6FC9C86A8 CRC64;
MASLALNTQA DPEIELFVKA GSDGESIGNC PFCQRLFMIL WLKGVKFNVT TIDTARKPEE
LKDLAPGTNP PFLIYNKELK TDFIKIEEFL EKTLAPPRYP HLSPKYKESF DVGCNLFAKF
SAYIKNTQKE ANKNFEKSLL REFKRLDDYL NTPLLDEIDP DSTEERTLSR RLFLDGDQLT
LADCSLLPKL NIIKVAAKKY RDFDIPAEFS GVWRYLHNAY AREEFAHTCP EDKEIENTYA
SVAKQ