ID   CLIC2_RAT               Reviewed;         245 AA.
AC   Q5M883;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Chloride intracellular channel protein 2;
GN   Name=Clic2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Can insert into membranes and form chloride ion channels.
CC       Channel activity depends on the pH. Membrane insertion seems to be
CC       redox-regulated and may occur only under oxydizing conditions.
CC       Modulates the activity of RYR2 and inhibits calcium influx (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. Interacts with TRAPPC2 and RYR2 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}. Note=Exists both as soluble
CC       cytoplasmic protein and as membrane protein with probably a single
CC       transmembrane domain. {ECO:0000250}.
CC   -!- DOMAIN: Members of this family may change from a globular, soluble
CC       state to a state where the N-terminal domain is inserted into the
CC       membrane and functions as chloride channel. A conformation change of
CC       the N-terminal domain is thought to expose hydrophobic surfaces that
CC       trigger membrane insertion (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chloride channel CLIC family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC088182; AAH88182.1; -; mRNA.
DR   RefSeq; NP_001009651.1; NM_001009651.1.
DR   AlphaFoldDB; Q5M883; -.
DR   SMR; Q5M883; -.
DR   STRING; 10116.ENSRNOP00000000955; -.
DR   iPTMnet; Q5M883; -.
DR   PhosphoSitePlus; Q5M883; -.
DR   PaxDb; Q5M883; -.
DR   PRIDE; Q5M883; -.
DR   Ensembl; ENSRNOT00000000955; ENSRNOP00000000955; ENSRNOG00000000728.
DR   GeneID; 294141; -.
DR   KEGG; rno:294141; -.
DR   UCSC; RGD:1306580; rat.
DR   CTD; 1193; -.
DR   RGD; 1306580; Clic2.
DR   eggNOG; KOG1422; Eukaryota.
DR   GeneTree; ENSGT00940000161397; -.
DR   HOGENOM; CLU_061051_1_0_1; -.
DR   InParanoid; Q5M883; -.
DR   OMA; IHTCPED; -.
DR   OrthoDB; 974249at2759; -.
DR   PhylomeDB; Q5M883; -.
DR   TreeFam; TF315438; -.
DR   Reactome; R-RNO-2672351; Stimuli-sensing channels.
DR   Reactome; R-RNO-5578775; Ion homeostasis.
DR   PRO; PR:Q5M883; -.
DR   Proteomes; UP000002494; Chromosome 20.
DR   Bgee; ENSRNOG00000000728; Expressed in spleen and 18 other tissues.
DR   Genevisible; Q5M883; RN.
DR   GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005254; F:chloride channel activity; IBA:GO_Central.
DR   GO; GO:0004602; F:glutathione peroxidase activity; ISO:RGD.
DR   GO; GO:0005244; F:voltage-gated ion channel activity; IEA:UniProtKB-KW.
DR   GO; GO:0006821; P:chloride transport; IBA:GO_Central.
DR   GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR   GO; GO:0060315; P:negative regulation of ryanodine-sensitive calcium-release channel activity; ISO:RGD.
DR   GO; GO:0051099; P:positive regulation of binding; ISO:RGD.
DR   GO; GO:0010880; P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; ISO:RGD.
DR   InterPro; IPR002946; CLIC.
DR   InterPro; IPR030253; CLIC-2.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR43920:SF4; PTHR43920:SF4; 1.
DR   Pfam; PF13409; GST_N_2; 1.
DR   PRINTS; PR01263; INTCLCHANNEL.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   TIGRFAMs; TIGR00862; O-ClC; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
PE   2: Evidence at transcript level;
KW   Chloride; Chloride channel; Cytoplasm; Disulfide bond; Ion channel;
KW   Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport; Voltage-gated channel.
FT   CHAIN           1..245
FT                   /note="Chloride intracellular channel protein 2"
FT                   /id="PRO_0000144206"
FT   TRANSMEM        32..52
FT                   /note="Helical; Note=After insertion into the membrane"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          99..239
FT                   /note="GST C-terminal"
FT   REGION          1..96
FT                   /note="Required for insertion into the membrane"
FT                   /evidence="ECO:0000250"
FT   DISULFID        30..33
FT                   /note="In soluble form"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   245 AA;  28161 MW;  DE6379E6FC9C86A8 CRC64;
     MASLALNTQA DPEIELFVKA GSDGESIGNC PFCQRLFMIL WLKGVKFNVT TIDTARKPEE
     LKDLAPGTNP PFLIYNKELK TDFIKIEEFL EKTLAPPRYP HLSPKYKESF DVGCNLFAKF
     SAYIKNTQKE ANKNFEKSLL REFKRLDDYL NTPLLDEIDP DSTEERTLSR RLFLDGDQLT
     LADCSLLPKL NIIKVAAKKY RDFDIPAEFS GVWRYLHNAY AREEFAHTCP EDKEIENTYA
     SVAKQ