ID   CLINK_BBTVA             Reviewed;         161 AA.
AC   Q65389;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 45.
DE   RecName: Full=Cell cycle link protein;
DE            Short=Clink;
GN   Name=DNA-C; Synonyms=C5;
OS   Banana bunchy top virus (isolate Autralia) (BBTV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes;
OC   Mulpavirales; Nanoviridae; Babuvirus.
OX   NCBI_TaxID=645099;
OH   NCBI_TaxID=4640; Musa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7782775; DOI=10.1099/0022-1317-76-6-1471;
RA   Burns T.M., Harding R.M., Dale J.L.;
RT   "The genome organization of banana bunchy top virus: analysis of six ssDNA
RT   components.";
RL   J. Gen. Virol. 76:1471-1482(1995).
RN   [2]
RP   INTERACTION WITH HUMAN RB1 AND WHEAT RBR2.
RX   PubMed=10640570; DOI=10.1099/0022-1317-81-1-299;
RA   Wanitchakorn R., Hafner G.J., Harding R.M., Dale J.L.;
RT   "Functional analysis of proteins encoded by banana bunchy top virus DNA-4
RT   to -6.";
RL   J. Gen. Virol. 81:299-306(2000).
RN   [3]
RP   FUNCTION.
RX   PubMed=21161359; DOI=10.1007/s11262-010-0559-3;
RA   Amin I., Ilyas M., Qazi J., Bashir R., Yadav J.S., Mansoor S.,
RA   Fauquet C.M., Briddon R.W.;
RT   "Identification of a major pathogenicity determinant and suppressors of RNA
RT   silencing encoded by a South Pacific isolate of Banana bunchy top virus
RT   originating from Pakistan.";
RL   Virus Genes 42:272-281(2011).
CC   -!- FUNCTION: Interacts with and disrupts the function of host
CC       retinoblastoma-related proteins RBR, which are key regulators of the
CC       cell cycle. Induces transcriptional activation of E2F-regulated S-phase
CC       and G2/M-phase-specific genes. Inactivation of the ability of RBR to
CC       arrest the cell cycle leads to the stimulation of viral DNA replication
CC       (By similarity). Acts as a suppressor of RNA-mediated gene silencing,
CC       also known as post-transcriptional gene silencing (PTGS), a mechanism
CC       of plant viral defense that limits the accumulation of viral RNAs.
CC       {ECO:0000250, ECO:0000269|PubMed:21161359}.
CC   -!- SUBUNIT: Interacts with host SKP1. Interacts (via LXCXE domain) with
CC       host retinoblastoma-related protein 2 (RBR2). Interacts (via LXCXE
CC       domain) with human RB1. Interacts (via LXCXE domain) with
CC       retinoblastoma-related proteins (RBR) (Probable).
CC       {ECO:0000305|PubMed:10640570}.
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DR   EMBL; L41578; AAA87371.1; -; Genomic_DNA.
DR   RefSeq; NP_604480.1; NC_003477.1.
DR   GeneID; 963870; -.
DR   KEGG; vg:963870; -.
DR   Proteomes; UP000002339; Genome.
DR   InterPro; IPR045832; Clink.
DR   Pfam; PF19411; Clink; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome; Suppressor of RNA silencing.
FT   CHAIN           1..161
FT                   /note="Cell cycle link protein"
FT                   /id="PRO_0000378517"
FT   REGION          9..21
FT                   /note="Binding to host SKP1 protein"
FT                   /evidence="ECO:0000255"
FT   MOTIF           111..115
FT                   /note="LXCXE motif, interaction with host RBR"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   161 AA;  18972 MW;  3A83F2AB434AD7AD CRC64;
     MEFWESSAMP DDVKREIKEI YWEDRKKLLF CQKLKSYVRR ILVYGDQEDA LAGVKDMKTS
     IIRYSEYLKK PCVVICCVSN KSIVYRLNSM VFFYHEYLEE LGGDYSVYQD LYCDEVLSSS
     STEEEDVGVI YRNVIMASTQ EKFSWSDCQQ IVISDYDVTL L