ID   CLINK_FBNY1             Reviewed;         169 AA.
AC   Q9WIJ4;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   23-FEB-2022, entry version 57.
DE   RecName: Full=Cell cycle link protein;
DE            Short=Clink;
GN   Name=DNA-C; Synonyms=C10;
OS   Faba bean necrotic yellows virus (isolate Egyptian EV1-93) (FBNYV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes;
OC   Mulpavirales; Nanoviridae; Nanovirus.
OX   NCBI_TaxID=291603;
OH   NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH   NCBI_TaxID=3864; Lens culinaris (Lentil) (Cicer lens).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9880028; DOI=10.1099/0022-1317-79-12-3101;
RA   Katul L., Timchenko T., Gronenborn B., Vetten H.J.;
RT   "Ten distinct circular ssDNA components, four of which encode putative
RT   replication-associated proteins, are associated with the faba bean necrotic
RT   yellows virus genome.";
RL   J. Gen. Virol. 79:3101-3109(1998).
RN   [2]
RP   INTERACTION WITH MEDICAGO SATIVA SKP1 AND ZEA MAYS RBR1, AND MUTAGENESIS OF
RP   PRO-10; ILE-17; CYS-112 AND GLU-114.
RX   PubMed=10708410; DOI=10.1128/jvi.74.7.2967-2972.2000;
RA   Aronson M.N., Meyer A.D., Gyorgyey J., Katul L., Vetten H.J.,
RA   Gronenborn B., Timchenko T.;
RT   "Clink, a nanovirus-encoded protein, binds both pRB and SKP1.";
RL   J. Virol. 74:2967-2972(2000).
RN   [3]
RP   FUNCTION.
RX   PubMed=17267511; DOI=10.1128/jvi.02103-06;
RA   Lageix S., Catrice O., Deragon J.-M., Gronenborn B., Pelissier T.,
RA   Ramirez B.C.;
RT   "The nanovirus-encoded Clink protein affects plant cell cycle regulation
RT   through interaction with the retinoblastoma-related protein.";
RL   J. Virol. 81:4177-4185(2007).
CC   -!- FUNCTION: Interacts with and disrupts the function of host
CC       retinoblastoma-related proteins RBR, which are key regulators of the
CC       cell cycle. Induces transcriptional activation of E2F-regulated S-phase
CC       and G2/M-phase-specific genes. Inactivation of the ability of RBR to
CC       arrest the cell cycle leads to the stimulation of viral DNA
CC       replication. {ECO:0000269|PubMed:17267511}.
CC   -!- SUBUNIT: Interacts with host SKP1. Interacts (via LXCXE domain) with
CC       host retinoblastoma-related protein 1 (RBR1). Interacts (via LXCXE
CC       domain) with retinoblastoma-related proteins (RBR) (Probable).
CC       {ECO:0000305|PubMed:10708410}.
CC   -!- SIMILARITY: Belongs to the nanovirus Clink protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ132179; CAB44019.1; -; Genomic_DNA.
DR   RefSeq; NP_619566.1; NC_003559.1.
DR   SMR; Q9WIJ4; -.
DR   GeneID; 993372; -.
DR   KEGG; vg:993372; -.
DR   Proteomes; UP000008665; Genome.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome.
FT   CHAIN           1..169
FT                   /note="Cell cycle link protein"
FT                   /id="PRO_0000338625"
FT   REGION          9..22
FT                   /note="Binding to host SKP1 protein"
FT                   /evidence="ECO:0000255"
FT   MOTIF           110..114
FT                   /note="LXCXE motif, interaction with host RBR"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         10
FT                   /note="P->L: Loss of binding to host SKP1. No effect on
FT                   viral replication stimulation."
FT                   /evidence="ECO:0000269|PubMed:10708410"
FT   MUTAGEN         17
FT                   /note="I->A: Loss of binding to host SKP1. No effect on
FT                   viral replication stimulation."
FT                   /evidence="ECO:0000269|PubMed:10708410"
FT   MUTAGEN         112
FT                   /note="C->R: Complete loss of viral replication
FT                   stimulation; when associated with A-114."
FT                   /evidence="ECO:0000269|PubMed:10708410"
FT   MUTAGEN         114
FT                   /note="E->A: Complete loss of viral replication
FT                   stimulation; when associated with R-112."
FT                   /evidence="ECO:0000269|PubMed:10708410"
SQ   SEQUENCE   169 AA;  19732 MW;  2A43EDB66575D535 CRC64;
     MGLKYFSHLP EELREKIVHD HLQQERKKEF LEKAIEDSCR RHVSLLKSDP SPSEMYSLSK
     FLDSLADYVG RQFNTRCLIK WRKDVPANIK FQVMEEQHLR LYGFLDMDDL SCRELLPPEE
     DDDITYEDGM IVNCSELDKL FAALGIRVVY ITVSNNCICT PLNKDIVIS