CLIP4_HUMAN
ID CLIP4_HUMAN Reviewed; 705 AA.
AC Q8N3C7; A0AV10; B2RMQ3; B7Z7N8; Q7Z4U3; Q96BR7; Q96MA5; Q9H7C0;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=CAP-Gly domain-containing linker protein 4;
DE AltName: Full=Restin-like protein 2;
GN Name=CLIP4; Synonyms=RSNL2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Guo J.H., Yu L.;
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 102-705 (ISOFORMS 3 AND 4).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Amygdala;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 48-705 (ISOFORM 2).
RC TISSUE=Lung, and Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 482-565.
RG RIKEN structural genomics initiative (RSGI);
RT "Crystal structure of the 2nd CAP-Gly domain in human restin-like protein 2
RT reveals a swapped-dimer.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- INTERACTION:
CC Q8N3C7; O75031: HSF2BP; NbExp=3; IntAct=EBI-5655540, EBI-7116203;
CC Q8N3C7; Q96AA8: JAKMIP2; NbExp=7; IntAct=EBI-5655540, EBI-752007;
CC Q8N3C7; Q9BVG8: KIFC3; NbExp=3; IntAct=EBI-5655540, EBI-2125614;
CC Q8N3C7; Q9BVG8-5: KIFC3; NbExp=3; IntAct=EBI-5655540, EBI-14069005;
CC Q8N3C7; Q9BRK4: LZTS2; NbExp=3; IntAct=EBI-5655540, EBI-741037;
CC Q8N3C7; P43365: MAGEA12; NbExp=6; IntAct=EBI-5655540, EBI-749530;
CC Q8N3C7; Q14244: MAP7; NbExp=3; IntAct=EBI-5655540, EBI-2211064;
CC Q8N3C7; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-5655540, EBI-16439278;
CC Q8N3C7; Q9NPC8: SIX2; NbExp=3; IntAct=EBI-5655540, EBI-12695166;
CC Q8N3C7; Q13049: TRIM32; NbExp=6; IntAct=EBI-5655540, EBI-742790;
CC Q8N3C7; Q15645: TRIP13; NbExp=3; IntAct=EBI-5655540, EBI-358993;
CC Q8N3C7; Q8NC26: ZNF114; NbExp=7; IntAct=EBI-5655540, EBI-10265237;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8N3C7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N3C7-2; Sequence=VSP_012971, VSP_012972;
CC Name=3;
CC IsoId=Q8N3C7-3; Sequence=VSP_012972;
CC Name=4;
CC IsoId=Q8N3C7-4; Sequence=VSP_012969, VSP_012970;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB14974.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF433661; AAP97312.1; -; mRNA.
DR EMBL; AK024722; BAB14974.1; ALT_INIT; mRNA.
DR EMBL; AK057267; BAB71403.1; -; mRNA.
DR EMBL; AK302325; BAH13674.1; -; mRNA.
DR EMBL; AL834443; CAD39103.1; -; mRNA.
DR EMBL; CH471053; EAX00510.1; -; Genomic_DNA.
DR EMBL; BC015310; AAH15310.2; -; mRNA.
DR EMBL; BC126167; AAI26168.1; -; mRNA.
DR EMBL; BC136331; AAI36332.1; -; mRNA.
DR CCDS; CCDS1770.1; -. [Q8N3C7-1]
DR CCDS; CCDS74502.1; -. [Q8N3C7-3]
DR RefSeq; NP_001274456.1; NM_001287527.1. [Q8N3C7-1]
DR RefSeq; NP_001274457.1; NM_001287528.1. [Q8N3C7-3]
DR RefSeq; NP_078968.3; NM_024692.5. [Q8N3C7-1]
DR RefSeq; XP_005264619.1; XM_005264562.2. [Q8N3C7-1]
DR RefSeq; XP_005264620.1; XM_005264563.1. [Q8N3C7-1]
DR RefSeq; XP_006712167.1; XM_006712104.2. [Q8N3C7-1]
DR RefSeq; XP_011531411.1; XM_011533109.1. [Q8N3C7-1]
DR RefSeq; XP_016860447.1; XM_017004958.1. [Q8N3C7-1]
DR PDB; 2Z0W; X-ray; 2.50 A; A=482-564.
DR PDBsum; 2Z0W; -.
DR AlphaFoldDB; Q8N3C7; -.
DR SMR; Q8N3C7; -.
DR BioGRID; 122857; 44.
DR IntAct; Q8N3C7; 34.
DR STRING; 9606.ENSP00000327009; -.
DR GlyGen; Q8N3C7; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8N3C7; -.
DR PhosphoSitePlus; Q8N3C7; -.
DR BioMuta; CLIP4; -.
DR DMDM; 60390950; -.
DR EPD; Q8N3C7; -.
DR jPOST; Q8N3C7; -.
DR MassIVE; Q8N3C7; -.
DR MaxQB; Q8N3C7; -.
DR PaxDb; Q8N3C7; -.
DR PeptideAtlas; Q8N3C7; -.
DR PRIDE; Q8N3C7; -.
DR ProteomicsDB; 71789; -. [Q8N3C7-1]
DR ProteomicsDB; 71790; -. [Q8N3C7-2]
DR ProteomicsDB; 71791; -. [Q8N3C7-3]
DR ProteomicsDB; 71792; -. [Q8N3C7-4]
DR TopDownProteomics; Q8N3C7-4; -. [Q8N3C7-4]
DR Antibodypedia; 51158; 129 antibodies from 24 providers.
DR DNASU; 79745; -.
DR Ensembl; ENST00000320081.10; ENSP00000327009.5; ENSG00000115295.21. [Q8N3C7-1]
DR Ensembl; ENST00000401605.5; ENSP00000384242.1; ENSG00000115295.21. [Q8N3C7-3]
DR Ensembl; ENST00000404424.5; ENSP00000385594.1; ENSG00000115295.21. [Q8N3C7-1]
DR Ensembl; ENST00000690063.1; ENSP00000508552.1; ENSG00000115295.21. [Q8N3C7-1]
DR Ensembl; ENST00000693264.1; ENSP00000509159.1; ENSG00000115295.21. [Q8N3C7-1]
DR GeneID; 79745; -.
DR KEGG; hsa:79745; -.
DR MANE-Select; ENST00000320081.10; ENSP00000327009.5; NM_024692.6; NP_078968.3.
DR UCSC; uc002rmu.5; human. [Q8N3C7-1]
DR CTD; 79745; -.
DR DisGeNET; 79745; -.
DR GeneCards; CLIP4; -.
DR HGNC; HGNC:26108; CLIP4.
DR HPA; ENSG00000115295; Low tissue specificity.
DR neXtProt; NX_Q8N3C7; -.
DR OpenTargets; ENSG00000115295; -.
DR PharmGKB; PA162382440; -.
DR VEuPathDB; HostDB:ENSG00000115295; -.
DR eggNOG; KOG0241; Eukaryota.
DR eggNOG; KOG4568; Eukaryota.
DR GeneTree; ENSGT00940000157706; -.
DR HOGENOM; CLU_023687_1_0_1; -.
DR InParanoid; Q8N3C7; -.
DR OMA; RCRWTNM; -.
DR OrthoDB; 482616at2759; -.
DR PhylomeDB; Q8N3C7; -.
DR TreeFam; TF326096; -.
DR PathwayCommons; Q8N3C7; -.
DR SignaLink; Q8N3C7; -.
DR BioGRID-ORCS; 79745; 6 hits in 1071 CRISPR screens.
DR ChiTaRS; CLIP4; human.
DR EvolutionaryTrace; Q8N3C7; -.
DR GenomeRNAi; 79745; -.
DR Pharos; Q8N3C7; Tdark.
DR PRO; PR:Q8N3C7; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8N3C7; protein.
DR Bgee; ENSG00000115295; Expressed in secondary oocyte and 181 other tissues.
DR ExpressionAtlas; Q8N3C7; baseline and differential.
DR Genevisible; Q8N3C7; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0035371; C:microtubule plus-end; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051010; F:microtubule plus-end binding; IBA:GO_Central.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.30.190; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR036859; CAP-Gly_dom_sf.
DR InterPro; IPR000938; CAP-Gly_domain.
DR InterPro; IPR028397; CLIP4.
DR PANTHER; PTHR18916:SF32; PTHR18916:SF32; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF01302; CAP_GLY; 3.
DR SMART; SM00248; ANK; 3.
DR SMART; SM01052; CAP_GLY; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF74924; SSF74924; 3.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS00845; CAP_GLY_1; 2.
DR PROSITE; PS50245; CAP_GLY_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; ANK repeat; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..705
FT /note="CAP-Gly domain-containing linker protein 4"
FT /id="PRO_0000083529"
FT REPEAT 65..101
FT /note="ANK 1"
FT REPEAT 149..180
FT /note="ANK 2"
FT REPEAT 186..215
FT /note="ANK 3"
FT DOMAIN 303..345
FT /note="CAP-Gly 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT DOMAIN 505..547
FT /note="CAP-Gly 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT DOMAIN 644..686
FT /note="CAP-Gly 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT REGION 391..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 391..408
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 557
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66HD5"
FT MOD_RES 609
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8CI96"
FT VAR_SEQ 1..150
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_012969"
FT VAR_SEQ 468..705
FT /note="LNSSATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIEL
FT EKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQNHSYPGFRR
FT SFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEM
FT GTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGIN
FT GSKLVDENC -> IYGFFNQAFLVFFILVCLFEFLSNIYSK (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_012970"
FT VAR_SEQ 511
FT /note="P -> PVL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.1"
FT /id="VSP_012971"
FT VAR_SEQ 600..705
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334, ECO:0000303|Ref.1"
FT /id="VSP_012972"
FT VARIANT 486
FT /note="R -> L (in dbSNP:rs3100246)"
FT /id="VAR_048675"
FT VARIANT 613
FT /note="T -> P (in dbSNP:rs34327508)"
FT /id="VAR_048676"
FT CONFLICT 321
FT /note="E -> G (in Ref. 2; BAB14974)"
FT /evidence="ECO:0000305"
FT STRAND 499..505
FT /evidence="ECO:0007829|PDB:2Z0W"
FT STRAND 508..512
FT /evidence="ECO:0007829|PDB:2Z0W"
FT STRAND 514..522
FT /evidence="ECO:0007829|PDB:2Z0W"
FT STRAND 524..530
FT /evidence="ECO:0007829|PDB:2Z0W"
FT STRAND 533..536
FT /evidence="ECO:0007829|PDB:2Z0W"
FT STRAND 542..546
FT /evidence="ECO:0007829|PDB:2Z0W"
FT HELIX 548..550
FT /evidence="ECO:0007829|PDB:2Z0W"
SQ SEQUENCE 705 AA; 76317 MW; 2BE72B2DD2206F12 CRC64;
MTIEDLPDFP LEGNPLFGRY PFIFSASDTP VIFSISAAPM PSDCEFSFFD PNDASCQEIL
FDPKTSVSEL FAILRQWVPQ VQQNIDIIGN EILKRGCNVN DRDGLTDMTL LHYTCKSGAH
GIGDVETAVK FATQLIDLGA DISLRSRWTN MNALHYAAYF DVPELIRVIL KTSKPKDVDA
TCSDFNFGTA LHIAAYNLCA GAVKCLLEQG ANPAFRNDKG QIPADVVPDP VDMPLEMADA
AATAKEIKQM LLDAVPLSCN ISKAMLPNYD HVTGKAMLTS LGLKLGDRVV IAGQKVGTLR
FCGTTEFASG QWAGIELDEP EGKNNGSVGK VQYFKCAPKY GIFAPLSKIS KAKGRRKNIT
HTPSTKAAVP LIRSQKIDVA HVTSKVNTGL MTSKKDSASE STLSLPPGEE LKTVTEKDVA
LLGSVSSCSS TSSLEHRQSY PKKQNAISSN KKTMSKSPSL SSRASAGLNS SATSTANNSR
CEGELRLGER VLVVGQRLGT IRFFGTTNFA PGYWYGIELE KPHGKNDGSV GGVQYFSCSP
RYGIFAPPSR VQRVTDSLDT LSEISSNKQN HSYPGFRRSF STTSASSQKE INRRNAFSKS
KAALRRSWSS TPTAGGIEGS VKLHEGSQVL LTSSNEMGTV RYVGPTDFAS GIWLGLELRS
AKGKNDGSVG DKRYFTCKPN HGVLVRPSRV TYRGINGSKL VDENC
