CLIP4_MOUSE
ID CLIP4_MOUSE Reviewed; 704 AA.
AC Q8CI96; Q8BW09; Q921Q4; Q9D2S6;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=CAP-Gly domain-containing linker protein 4;
DE AltName: Full=Restin-like protein 2;
GN Name=Clip4; Synonyms=Rsnl2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary gland, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-608, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP STRUCTURE BY NMR OF 266-354 AND 617-696.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the 1st and the 3rd CAP-Gly domain.";
RL Submitted (NOV-2004) to the PDB data bank.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8CI96-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CI96-2; Sequence=VSP_012975, VSP_012976;
CC Name=3;
CC IsoId=Q8CI96-3; Sequence=VSP_012973, VSP_012974;
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DR EMBL; AK018895; BAB31478.1; -; mRNA.
DR EMBL; AK075758; BAC35936.1; -; mRNA.
DR EMBL; BC011280; AAH11280.1; -; mRNA.
DR EMBL; BC035226; AAH35226.1; -; mRNA.
DR CCDS; CCDS37687.1; -. [Q8CI96-1]
DR CCDS; CCDS89149.1; -. [Q8CI96-2]
DR RefSeq; NP_084455.2; NM_030179.3. [Q8CI96-1]
DR RefSeq; NP_780587.1; NM_175378.1. [Q8CI96-2]
DR RefSeq; XP_006525206.1; XM_006525143.3. [Q8CI96-1]
DR RefSeq; XP_006525207.1; XM_006525144.2. [Q8CI96-1]
DR RefSeq; XP_006525208.1; XM_006525145.3. [Q8CI96-1]
DR RefSeq; XP_006525209.1; XM_006525146.1. [Q8CI96-1]
DR RefSeq; XP_006525217.1; XM_006525154.2. [Q8CI96-2]
DR PDB; 1WHJ; NMR; -; A=266-354.
DR PDB; 1WHK; NMR; -; A=617-694.
DR PDBsum; 1WHJ; -.
DR PDBsum; 1WHK; -.
DR AlphaFoldDB; Q8CI96; -.
DR SMR; Q8CI96; -.
DR STRING; 10090.ENSMUSP00000024854; -.
DR iPTMnet; Q8CI96; -.
DR PhosphoSitePlus; Q8CI96; -.
DR jPOST; Q8CI96; -.
DR MaxQB; Q8CI96; -.
DR PaxDb; Q8CI96; -.
DR PRIDE; Q8CI96; -.
DR ProteomicsDB; 283521; -. [Q8CI96-1]
DR ProteomicsDB; 283522; -. [Q8CI96-2]
DR ProteomicsDB; 283523; -. [Q8CI96-3]
DR Antibodypedia; 51158; 129 antibodies from 24 providers.
DR DNASU; 78785; -.
DR Ensembl; ENSMUST00000024854; ENSMUSP00000024854; ENSMUSG00000024059. [Q8CI96-1]
DR Ensembl; ENSMUST00000229952; ENSMUSP00000155140; ENSMUSG00000024059. [Q8CI96-2]
DR Ensembl; ENSMUST00000230427; ENSMUSP00000155130; ENSMUSG00000024059. [Q8CI96-3]
DR GeneID; 78785; -.
DR KEGG; mmu:78785; -.
DR UCSC; uc008dmw.2; mouse. [Q8CI96-3]
DR UCSC; uc008dmx.2; mouse. [Q8CI96-1]
DR UCSC; uc012awx.1; mouse. [Q8CI96-2]
DR CTD; 79745; -.
DR MGI; MGI:1919100; Clip4.
DR VEuPathDB; HostDB:ENSMUSG00000024059; -.
DR eggNOG; KOG0241; Eukaryota.
DR eggNOG; KOG4568; Eukaryota.
DR GeneTree; ENSGT00940000157706; -.
DR HOGENOM; CLU_023687_1_0_1; -.
DR InParanoid; Q8CI96; -.
DR OMA; NPFDEGE; -.
DR OrthoDB; 482616at2759; -.
DR PhylomeDB; Q8CI96; -.
DR TreeFam; TF326096; -.
DR BioGRID-ORCS; 78785; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Clip4; mouse.
DR EvolutionaryTrace; Q8CI96; -.
DR PRO; PR:Q8CI96; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8CI96; protein.
DR Bgee; ENSMUSG00000024059; Expressed in seminiferous tubule of testis and 227 other tissues.
DR ExpressionAtlas; Q8CI96; baseline and differential.
DR Genevisible; Q8CI96; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0035371; C:microtubule plus-end; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051010; F:microtubule plus-end binding; IBA:GO_Central.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 2.30.30.190; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR036859; CAP-Gly_dom_sf.
DR InterPro; IPR000938; CAP-Gly_domain.
DR InterPro; IPR028397; CLIP4.
DR PANTHER; PTHR18916:SF32; PTHR18916:SF32; 1.
DR Pfam; PF01302; CAP_GLY; 3.
DR SMART; SM00248; ANK; 3.
DR SMART; SM01052; CAP_GLY; 3.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF74924; SSF74924; 3.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS00845; CAP_GLY_1; 2.
DR PROSITE; PS50245; CAP_GLY_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; ANK repeat; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..704
FT /note="CAP-Gly domain-containing linker protein 4"
FT /id="PRO_0000083530"
FT REPEAT 65..101
FT /note="ANK 1"
FT REPEAT 149..180
FT /note="ANK 2"
FT REPEAT 186..215
FT /note="ANK 3"
FT DOMAIN 303..345
FT /note="CAP-Gly 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT DOMAIN 504..546
FT /note="CAP-Gly 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT DOMAIN 643..685
FT /note="CAP-Gly 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT REGION 353..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 379..407
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 556
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66HD5"
FT MOD_RES 608
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 217..219
FT /note="NDK -> VRD (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_012973"
FT VAR_SEQ 220..704
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_012974"
FT VAR_SEQ 341..387
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_012975"
FT VAR_SEQ 599..704
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_012976"
FT CONFLICT 30
FT /note="S -> Y (in Ref. 1; BAC35936)"
FT /evidence="ECO:0000305"
FT CONFLICT 44
FT /note="C -> S (in Ref. 1; BAC35936)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="A -> S (in Ref. 1; BAC35936)"
FT /evidence="ECO:0000305"
FT CONFLICT 495
FT /note="Q -> K (in Ref. 1; BAC35936)"
FT /evidence="ECO:0000305"
FT STRAND 268..270
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 273..275
FT /evidence="ECO:0007829|PDB:1WHJ"
FT HELIX 278..281
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 288..291
FT /evidence="ECO:0007829|PDB:1WHJ"
FT TURN 292..294
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 295..304
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 306..308
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 310..320
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 323..328
FT /evidence="ECO:0007829|PDB:1WHJ"
FT TURN 338..340
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 341..345
FT /evidence="ECO:0007829|PDB:1WHJ"
FT HELIX 346..348
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 349..354
FT /evidence="ECO:0007829|PDB:1WHJ"
FT STRAND 627..633
FT /evidence="ECO:0007829|PDB:1WHK"
FT STRAND 636..639
FT /evidence="ECO:0007829|PDB:1WHK"
FT STRAND 646..656
FT /evidence="ECO:0007829|PDB:1WHK"
FT STRAND 658..660
FT /evidence="ECO:0007829|PDB:1WHK"
FT STRAND 666..668
FT /evidence="ECO:0007829|PDB:1WHK"
FT HELIX 686..688
FT /evidence="ECO:0007829|PDB:1WHK"
SQ SEQUENCE 704 AA; 75789 MW; 615B49B7A536B918 CRC64;
MTIEDLPDIP LEGSSLIGRY PFLFTGSDTS VIFSISAAPM PSDCEFSFFD PNDASCQEIL
FDPKTSVSEL FAILRQWVPQ VQQNIDIIGN EILKRGCNVN DRDGLTDMTL LHYTCKSGAH
GIGDIETAVK FAAQLIDLGA DASLRSRWTN MNALHYASYF DVPELIRVIL KTSKPKDVDA
TCSDFNFGTA LHIAAHNLCA GAVKTLLELG ANPAFRNDKG QIPAEVVPDP VDMPLEMADA
AAIAKEIKQM LLDAVPLPCT SAKAVLPNSD HTTSRAMLTS LGLKLGDRVV IAGQKVGTLR
FCGTTEFASG QWAGIELDEP EGKNNGSVGR VQYFKCAPKY GIFAPLSKIS KLKDGRKTTT
HTPSTRATPH ARSQKVDVAH FTSRVNSGLT TSKKETASES TLTLPPSEEP KTVAENDAAQ
PGSMSSSSSS SSLDHKQSYP KKLTTSSGGK KTLSKSPSLP SRASAGLKSS ATSAANNSHH
EGALHLGERV LVVGQRVGTI KFFGTTNFAP GYWYGIELEK PHGKNDGSVG GVQYFSCSPR
YGIFAPPSRV QRLSDSLDTL SEISSNKQNH SYPGFRRSFS TTSASSQKEI NRRNAFAKTK
TTLRRSWSSS TTAGGLEGTV KLHEGSQVLL TSSNEMATVR YVGPTDFASG IWLGLELRSA
KGKNDGAVGD KRYFTCKPNY GVLVRPSRVT YRGISGSKLI DENC
