CLPB1_ORYSJ
ID CLPB1_ORYSJ Reviewed; 912 AA.
AC Q6F2Y7; A0A0P0WPL5; Q0DGP3; Q8L6I4; Q8W2B5;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Chaperone protein ClpB1;
DE AltName: Full=ATP-dependent Clp protease ATP-binding subunit ClpB homolog 1;
DE AltName: Full=Casein lytic proteinase B1;
DE AltName: Full=Heat shock protein 101;
GN Name=CLPB1; Synonyms=HSP101;
GN OrderedLocusNames=Os05g0519700, LOC_Os05g44340;
GN ORFNames=OsJ_19232, P0483D07.3, P0599F04.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=12650620; DOI=10.1023/a:1022324920316;
RA Agarwal M., Sahi C., Katiyar-Agarwal S., Agarwal S., Young T., Gallie D.R.,
RA Sharma V.M., Ganesan K., Grover A.;
RT "Molecular characterization of rice hsp101: complementation of yeast hsp104
RT mutation by disaggregation of protein granules and differential expression
RT in indica and japonica rice types.";
RL Plant Mol. Biol. 51:543-553(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP DEVELOPMENTAL STAGE, AND INDUCTION BY HEAT STRESS.
RX PubMed=20141629; DOI=10.1186/1471-2164-11-95;
RA Singh A., Singh U., Mittal D., Grover A.;
RT "Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes.";
RL BMC Genomics 11:95-95(2010).
CC -!- FUNCTION: Molecular chaperone involved in heat stress response. May
CC play a role in resolubilization of protein aggregates after heat shock.
CC {ECO:0000269|PubMed:12650620}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing embryos and dry seeds, and
CC decreases rapidly during seed germination.
CC {ECO:0000269|PubMed:20141629}.
CC -!- INDUCTION: Expressed within five minutes of heat shock at 42 and 45
CC degrees Celsius in one week-old seedlings. Maximal level of expression
CC after 30 minutes and decrease of expression after four hours at 45
CC degrees Celsius. No induction detected after salt stress, low
CC temperature, desiccation, or abscisic acid (ABA) treatment. Protein
CC expressed up to 72 hours in Indica rice and up to 96 hours in Japonica
CC rice after a 2 hours heat shock at 42 degrees Celsius.
CC {ECO:0000269|PubMed:12650620, ECO:0000269|PubMed:20141629}.
CC -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
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DR EMBL; AF332981; AAL57165.1; -; mRNA.
DR EMBL; AJ316025; CAC87117.1; -; Genomic_DNA.
DR EMBL; AC130611; AAT69657.1; -; Genomic_DNA.
DR EMBL; AC132491; AAU44265.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17980.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS94924.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE64390.1; -; Genomic_DNA.
DR EMBL; AK105433; BAG97245.1; -; mRNA.
DR RefSeq; XP_015640227.1; XM_015784741.1.
DR AlphaFoldDB; Q6F2Y7; -.
DR SMR; Q6F2Y7; -.
DR STRING; 4530.OS05T0519700-01; -.
DR PaxDb; Q6F2Y7; -.
DR PRIDE; Q6F2Y7; -.
DR EnsemblPlants; Os05t0519700-01; Os05t0519700-01; Os05g0519700.
DR EnsemblPlants; Os05t0519700-02; Os05t0519700-02; Os05g0519700.
DR GeneID; 4339343; -.
DR Gramene; Os05t0519700-01; Os05t0519700-01; Os05g0519700.
DR Gramene; Os05t0519700-02; Os05t0519700-02; Os05g0519700.
DR KEGG; osa:4339343; -.
DR eggNOG; KOG1051; Eukaryota.
DR HOGENOM; CLU_005070_4_2_1; -.
DR InParanoid; Q6F2Y7; -.
DR OMA; GPEHILM; -.
DR OrthoDB; 611758at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR ExpressionAtlas; Q6F2Y7; baseline and differential.
DR Genevisible; Q6F2Y7; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IGI:UniProtKB.
DR Gene3D; 1.10.1780.10; -; 1.
DR Gene3D; 3.40.50.300; -; 3.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR019489; Clp_ATPase_C.
DR InterPro; IPR036628; Clp_N_dom_sf.
DR InterPro; IPR004176; Clp_R_dom.
DR InterPro; IPR001270; ClpA/B.
DR InterPro; IPR018368; ClpA/B_CS1.
DR InterPro; IPR028299; ClpA/B_CS2.
DR InterPro; IPR041546; ClpA/ClpB_AAA_lid.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF07724; AAA_2; 1.
DR Pfam; PF17871; AAA_lid_9; 1.
DR Pfam; PF02861; Clp_N; 2.
DR Pfam; PF10431; ClpB_D2-small; 1.
DR PRINTS; PR00300; CLPPROTEASEA.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01086; ClpB_D2-small; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF81923; SSF81923; 1.
DR PROSITE; PS51903; CLP_R; 1.
DR PROSITE; PS00870; CLPAB_1; 1.
DR PROSITE; PS00871; CLPAB_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chaperone; Coiled coil; Cytoplasm; Nucleotide-binding;
KW Nucleus; Reference proteome; Repeat; Stress response.
FT CHAIN 1..912
FT /note="Chaperone protein ClpB1"
FT /id="PRO_0000191221"
FT DOMAIN 3..149
FT /note="Clp R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 6..75
FT /note="Repeat 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 87..149
FT /note="Repeat 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 166..412
FT /note="I"
FT REGION 533..724
FT /note="II"
FT COILED 406..502
FT /evidence="ECO:0000255"
FT BINDING 209..216
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 607..614
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 398
FT /note="D -> G (in Ref. 1; CAC87117)"
FT /evidence="ECO:0000305"
FT CONFLICT 401
FT /note="C -> R (in Ref. 1; CAC87117)"
FT /evidence="ECO:0000305"
FT CONFLICT 558
FT /note="K -> E (in Ref. 1; CAC87117)"
FT /evidence="ECO:0000305"
FT CONFLICT 678
FT /note="F -> Y (in Ref. 1; CAC87117)"
FT /evidence="ECO:0000305"
FT CONFLICT 791
FT /note="E -> G (in Ref. 1; CAC87117)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 912 AA; 100896 MW; 0A3B17065B7CA343 CRC64;
MNPDNFTHKT NEALVAAHEI ASEAGHAQLT PLHLVAALAA DKGGILRQAI SQASGGDAGA
PDSFERVVSG ALKKLPSQSP PPDSVPASTA LIKVIRRAQS AQKKRGDSHL AVDQLLLGLL
EDSLISDCLK EAGVSAARVR AELEKLRGGE GRKVESASGD TNFQALKTYG RDLVEQAGKL
DPVIGRDEEI RRVVRILSRR TKNNPVLIGE PGVGKTAVVE GLAQRIVRGD VPSNLLDVRL
IALDMGALVA GAKYRGEFEE RLKAVLKEVE EAEGKVILFI DEIHLVLGAG RTEGSMDAAN
LFKPMLARGQ LRCIGATTLE EYRKYVEKDA AFERRFQQVF VAEPSVPDTI SILRGLKEKY
EGHHGVRIQD RALVVAAQLS ARYIMGRHLP DKAIDLVDEA CANVRVQLDS QPEEIDNLER
KRIQLEVEHH ALEKEKDKAS KARLVEVKKE LDDLRDKLQP LTMKYRKEKE RIDEIRKLKQ
RREELQFTLQ EAERRMDLAR VADLKYGALQ EIDVAIAKLE SETGENLMLT ETVGPEQIAE
VVSRWTGIPV TRLGQNDKER LVGLADRLHQ RVVGQAEAVS AVAEAVLRSR AGLGRPQQPT
GSFLFLGPTG VGKTELAKAL AEQLFDDENL LVRIDMSEYM EQHSVARLIG APPGYVGHEE
GGQLTEQVRR RPYSVILFDE VEKAHVAVFN TLLQVLDDGR LTDGQGRTVD FRNTVIIMTS
NLGAEHLLAG MVGKNSMKVA RDLVMQEVRR HFRPELLNRL DEIVIFDPLS HEQLRKVARL
QMKDVAVRLA ERGVALAVTD AALDVILSLS YDPVYGARPI RRWIEKRVVT QLSKMLIQEE
IDENCTVYID AAPHKDELAY RVDNRGGLVN AETGQKSDIL IQVPNGAATG SDAAQAVKKM
RIMEDEDGMD EE
