CLPB2_ORYSJ
ID CLPB2_ORYSJ Reviewed; 978 AA.
AC Q75GT3; A0A0P0VZN5; Q75I57;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Chaperone protein ClpB2, chloroplastic;
DE AltName: Full=ATP-dependent Clp protease ATP-binding subunit ClpB homolog 2;
DE AltName: Full=Casein lytic proteinase B2;
DE Flags: Precursor;
GN Name=CLPB2; Synonyms=CLPB-C;
GN OrderedLocusNames=Os03g0426900, LOC_Os03g31300;
GN ORFNames=OSJNBa0020H02.1, OSJNBa0083F15.25;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=20141629; DOI=10.1186/1471-2164-11-95;
RA Singh A., Singh U., Mittal D., Grover A.;
RT "Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes.";
RL BMC Genomics 11:95-95(2010).
CC -!- FUNCTION: Molecular chaperone that may play a role in chloroplast
CC development.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:20141629}.
CC -!- INDUCTION: By heat and oxidative stresses.
CC {ECO:0000269|PubMed:20141629}.
CC -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
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DR EMBL; AC133398; AAR01771.1; -; Genomic_DNA.
DR EMBL; AC137999; AAS07199.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF96723.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12324.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84787.1; -; Genomic_DNA.
DR EMBL; AK069123; BAG91268.1; -; mRNA.
DR RefSeq; XP_015630429.1; XM_015774943.1.
DR AlphaFoldDB; Q75GT3; -.
DR SMR; Q75GT3; -.
DR STRING; 4530.OS03T0426900-01; -.
DR PaxDb; Q75GT3; -.
DR PRIDE; Q75GT3; -.
DR EnsemblPlants; Os03t0426900-01; Os03t0426900-01; Os03g0426900.
DR GeneID; 4333161; -.
DR Gramene; Os03t0426900-01; Os03t0426900-01; Os03g0426900.
DR KEGG; osa:4333161; -.
DR eggNOG; KOG1051; Eukaryota.
DR HOGENOM; CLU_005070_4_1_1; -.
DR InParanoid; Q75GT3; -.
DR OMA; SKMMQGE; -.
DR OrthoDB; 611758at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q75GT3; baseline and differential.
DR Genevisible; Q75GT3; OS.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0009532; C:plastid stroma; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0009658; P:chloroplast organization; IEA:EnsemblPlants.
DR GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR Gene3D; 1.10.1780.10; -; 1.
DR Gene3D; 3.40.50.300; -; 3.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR017730; Chaperonin_ClpB.
DR InterPro; IPR019489; Clp_ATPase_C.
DR InterPro; IPR036628; Clp_N_dom_sf.
DR InterPro; IPR004176; Clp_R_dom.
DR InterPro; IPR001270; ClpA/B.
DR InterPro; IPR018368; ClpA/B_CS1.
DR InterPro; IPR028299; ClpA/B_CS2.
DR InterPro; IPR041546; ClpA/ClpB_AAA_lid.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF07724; AAA_2; 1.
DR Pfam; PF17871; AAA_lid_9; 1.
DR Pfam; PF02861; Clp_N; 2.
DR Pfam; PF10431; ClpB_D2-small; 1.
DR PRINTS; PR00300; CLPPROTEASEA.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01086; ClpB_D2-small; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF81923; SSF81923; 1.
DR TIGRFAMs; TIGR03346; chaperone_ClpB; 1.
DR PROSITE; PS51903; CLP_R; 1.
DR PROSITE; PS00870; CLPAB_1; 1.
DR PROSITE; PS00871; CLPAB_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chaperone; Chloroplast; Nucleotide-binding; Plastid;
KW Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..76
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 77..978
FT /note="Chaperone protein ClpB2, chloroplastic"
FT /id="PRO_0000412577"
FT DOMAIN 85..229
FT /note="Clp R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 89..154
FT /note="Repeat 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 166..229
FT /note="Repeat 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 244..492
FT /note="I"
FT /evidence="ECO:0000250"
FT REGION 618..809
FT /note="II"
FT /evidence="ECO:0000250"
FT BINDING 289..296
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 692..699
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 978 AA; 108985 MW; C07DC7C69987083F CRC64;
MAAAPPLAAG LRPAMAAAQA PVVAAAWGVG ARRGAALSSS ARCRALRLSR GGGGGRDGWV
PPPVVGRMPP RTLSVRCAAS NGRITQQEFT EMAWQSIVSS PEVAKESKHQ IVETEHLMKS
LLEQRNGLAR RIFSKAGVDN TRLLDATEKF IQRQPKVLGE DPGSMLGRDL EALIQRARDF
KKEYGDSFVS VEHLVLGFAE DKRFGRQLFK DFQITVQSLK TAIESIRGKQ NVIDQDPEGK
YEALDKYGKD LTAMARQGKL DPVIGRDDEI RRCIQILSRR TKNNPVLIGE PGVGKTAIAE
GLAQRIVQGD VPQALTNRRL IALDMGALIA GAKYRGEFED RLKAVLKEVT DSDGQTILFI
DEIHTVVGAG ATNGAMDAGN LLKPMLGRGE LRCIGATTLD EYRKYIEKDP ALERRFQQVY
VDQPSVEDTI SILRGLRERY ELHHGVRISD SALVAAALLS DRYISGRFLP DKAIDLVDES
AAKLKMEITS KPTALDEIDR AVIKLEMERL SLTNDTDKAS RDRLSRIEAE LSLLKEKQKD
LTEQWEREKS VMTKIQSIKE EIDRVNVEIQ QAEREYDLNR AAELKYGSLN ALQRQLQTTE
KELDEYQSSG KSMLREEVTQ DDIAEIVSRW TGIPVSKLKQ SDREKLLYLE EELHKRVVGQ
DPAVKAVSEA IQRSRAGLSD PNRPIASFMF MGPTGVGKTE LAKALAAFMF NTEEAVVRID
MSEYMEKHSV SRLIGAPPGY VGYEEGGQLT EAVRRRPYSI ILFDEIEKAH GDVFNVFLQI
LDDGRVTDSQ GRKVSFTNSI IIMTSNVGSQ FILNMDEEGG STDSAYENIK KRVMDAARSV
FRPEFMNRID EYIVFKPLER EQINSIVKLQ LARVQKRIAD RKIKLEVSPG AVEFLGSLGY
DPNYGARPVK RVIQQYVENE LAKGILRGDF KDEDSILVDT QVTVPSNGQL PQQKLVFHKM
SEESAPAAAE DEKFLPAV
