ID   CLPB3_ORYSJ             Reviewed;         983 AA.
AC   Q0E3C8; A0A0N7KET5;
DT   21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 3.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Chaperone protein ClpB3, mitochondrial;
DE   AltName: Full=ATP-dependent Clp protease ATP-binding subunit ClpB homolog 3;
DE   AltName: Full=Casein lytic proteinase B3;
DE   Flags: Precursor;
GN   Name=CLPB3; Synonyms=CLPB-M;
GN   OrderedLocusNames=Os02g0181900, LOC_Os02g08490;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RG   The rice full-length cDNA consortium;
RT   "Oryza sativa full length cDNA.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=20141629; DOI=10.1186/1471-2164-11-95;
RA   Singh A., Singh U., Mittal D., Grover A.;
RT   "Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes.";
RL   BMC Genomics 11:95-95(2010).
CC   -!- FUNCTION: Molecular chaperone that may not be involved in heat stress
CC       response or tolerance.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:20141629}.
CC   -!- INDUCTION: By heat and oxidative stresses, and abscisic acid (ABA).
CC       {ECO:0000269|PubMed:20141629}.
CC   -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
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DR   EMBL; AP008208; BAF08010.2; -; Genomic_DNA.
DR   EMBL; AP014958; BAS77313.1; -; Genomic_DNA.
DR   EMBL; AK287906; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015627597.1; XM_015772111.1.
DR   AlphaFoldDB; Q0E3C8; -.
DR   SMR; Q0E3C8; -.
DR   STRING; 4530.OS02T0181900-01; -.
DR   PaxDb; Q0E3C8; -.
DR   PRIDE; Q0E3C8; -.
DR   EnsemblPlants; Os02t0181900-01; Os02t0181900-01; Os02g0181900.
DR   GeneID; 4328515; -.
DR   Gramene; Os02t0181900-01; Os02t0181900-01; Os02g0181900.
DR   KEGG; osa:4328515; -.
DR   eggNOG; KOG1051; Eukaryota.
DR   HOGENOM; CLU_005070_4_2_1; -.
DR   InParanoid; Q0E3C8; -.
DR   OMA; KRYITDH; -.
DR   OrthoDB; 611758at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q0E3C8; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR   Gene3D; 1.10.1780.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 3.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR017730; Chaperonin_ClpB.
DR   InterPro; IPR019489; Clp_ATPase_C.
DR   InterPro; IPR036628; Clp_N_dom_sf.
DR   InterPro; IPR004176; Clp_R_dom.
DR   InterPro; IPR001270; ClpA/B.
DR   InterPro; IPR018368; ClpA/B_CS1.
DR   InterPro; IPR028299; ClpA/B_CS2.
DR   InterPro; IPR041546; ClpA/ClpB_AAA_lid.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF07724; AAA_2; 1.
DR   Pfam; PF17871; AAA_lid_9; 1.
DR   Pfam; PF02861; Clp_N; 2.
DR   Pfam; PF10431; ClpB_D2-small; 1.
DR   PRINTS; PR00300; CLPPROTEASEA.
DR   SMART; SM00382; AAA; 2.
DR   SMART; SM01086; ClpB_D2-small; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF81923; SSF81923; 1.
DR   TIGRFAMs; TIGR03346; chaperone_ClpB; 1.
DR   PROSITE; PS51903; CLP_R; 1.
DR   PROSITE; PS00870; CLPAB_1; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chaperone; Coiled coil; Mitochondrion; Nucleotide-binding;
KW   Reference proteome; Repeat; Transit peptide.
FT   TRANSIT         1..87
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           88..983
FT                   /note="Chaperone protein ClpB3, mitochondrial"
FT                   /id="PRO_0000412578"
FT   DOMAIN          97..240
FT                   /note="Clp R"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          100..165
FT                   /note="Repeat 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          177..240
FT                   /note="Repeat 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          255..503
FT                   /note="I"
FT                   /evidence="ECO:0000250"
FT   REGION          629..820
FT                   /note="II"
FT                   /evidence="ECO:0000250"
FT   COILED          504..627
FT                   /evidence="ECO:0000255"
FT   BINDING         300..307
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         703..710
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        264
FT                   /note="E -> G (in Ref. 4; AK287906)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   983 AA;  109247 MW;  21728B99316C3ED3 CRC64;
     MSRATAVSRL ARAARAAAAA RRHHAGGRDP LRALASLAGD ASASAGGGAR RPAWFAPPMG
     RLGGGGLLVP PPPPQRRLFH PTQAARYSTS SSSQITPGEF TEMAWEGVVG AVDAARMSKQ
     QVVEAEHLMK ALLEQKDGLA RRIFSKAGID NTSVLQATDE FISRQPKVVG DTSGPIIGSS
     FVSILDNARK HKKEYADEFV SVEHILRAFT EDKRFGQQLF RDLKIGENEL KEAISAVRGS
     QRVTDQNPEG KYQALEKYGI DMTELARRGK LDPVIGRDDE VRRCIQILCR RTKNNPVIIG
     EPGVGKTAIA EGLAQRIVRG DVPEPLQNRK LISLDMGALL AGAKFQGQFE ERLKAVLKEI
     TASNGQIILF IDEIHTIVGA GAAGGAMDAG NLLKPMLGRG ELRCIGATTL DEYRKYIEKD
     AALERRFQQV YCGEPAVEDT ISILRGLRER YELHHGVKIS DGALVSAAVL SDRYITGRFL
     PDKAIDLVDE AAAKLKMEIT SKPIELDEVD REIIRLEMEK LSLKNDTDKA SKQRLSKLEA
     DLESLKQKQK NLSEHWEYEK SLMTRIRSIK EETDRVNLEI EAAEREYDLN RAAELKYGTL
     LSLQKQLEEA ENKLMEFQQS GKSMLREEVT DVDIAEIVSK WTGIPVSNLQ QSEKEKLLLL
     EDVLHKRVIG QDIAVKSVAN AIRRSRAGLS DPNRPIASLM FMGPTGVGKT ELGKTLAEFL
     FNTENALIRI DMSEYMEKHA VSRLVGAPPG YIGYGEGGQL TEAVRRRPYS VVLFDEIEKA
     HQDVFNILLQ LLDDGRITDS QGRTVSFTNC VIIMTSNIGS PLILDTLRNT SDSKEAVYEI
     MKKQVIDMAR QSFRPEFLNR IDEYIVFQPL DTTEINRIVE IQLNRVKNRL RQQKIHLQYT
     PEAVEHLGSL GFDPNYGARP VKRVIQQMVE NEIALSVLKG DFKEDDTVLV DVSSVAIAKG
     LAPQKKLVLQ RLENANLELV AND