CLPC_BACSU
ID CLPC_BACSU Reviewed; 810 AA.
AC P37571;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Negative regulator of genetic competence ClpC/MecB;
GN Name=clpC; Synonyms=mecB; OrderedLocusNames=BSU00860;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8016066; DOI=10.1073/pnas.91.13.5788;
RA Msadek T., Kunst F., Rapoport G.;
RT "MecB of Bacillus subtilis, a member of the ClpC ATPase family, is a
RT pleiotropic regulator controlling competence gene expression and growth at
RT high temperature.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:5788-5792(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-387.
RX PubMed=8195092; DOI=10.1128/jb.176.11.3360-3367.1994;
RA Krueger E., Voelker U., Hecker M.;
RT "Stress induction of clpC in Bacillus subtilis and its involvement in
RT stress tolerance.";
RL J. Bacteriol. 176:3360-3367(1994).
CC -!- FUNCTION: Competence gene repressor; required for cell growth at high
CC temperature. Negative regulator of comK expression. May interact with
CC MecA to negatively regulate comK.
CC -!- INTERACTION:
CC P37571; P37958: mecA; NbExp=11; IntAct=EBI-7349302, EBI-5254676;
CC -!- SIMILARITY: Belongs to the ClpA/ClpB family. ClpC subfamily.
CC {ECO:0000305}.
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DR EMBL; U02604; AAA19233.1; -; Genomic_DNA.
DR EMBL; D26185; BAA05320.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11862.1; -; Genomic_DNA.
DR EMBL; X75930; CAA53534.1; -; Genomic_DNA.
DR PIR; I40508; I40508.
DR RefSeq; NP_387967.1; NC_000964.3.
DR RefSeq; WP_003235011.1; NZ_JNCM01000029.1.
DR PDB; 2K77; NMR; -; A=1-145.
DR PDB; 2Y1Q; X-ray; 1.50 A; A=1-150.
DR PDB; 2Y1R; X-ray; 2.60 A; A/B/C/D/E/F/G/H=1-149.
DR PDB; 3J3R; EM; 9.40 A; A/B/C/D/E/F=1-810.
DR PDB; 3J3S; EM; 11.00 A; A/B/C/D/E/F=1-810.
DR PDB; 3J3T; EM; 9.00 A; A/B/C/D/E/F=1-810.
DR PDB; 3J3U; EM; 10.00 A; A/B/C/D/E/F=1-810.
DR PDB; 3PXG; X-ray; 3.65 A; A/B/C/D/E/F=1-485.
DR PDB; 3PXI; X-ray; 6.93 A; A/B/C=1-810.
DR PDB; 5HBN; X-ray; 1.60 A; A=1-150.
DR PDB; 7ABR; EM; 3.70 A; A/B/C/D/E/F=1-810.
DR PDBsum; 2K77; -.
DR PDBsum; 2Y1Q; -.
DR PDBsum; 2Y1R; -.
DR PDBsum; 3J3R; -.
DR PDBsum; 3J3S; -.
DR PDBsum; 3J3T; -.
DR PDBsum; 3J3U; -.
DR PDBsum; 3PXG; -.
DR PDBsum; 3PXI; -.
DR PDBsum; 5HBN; -.
DR PDBsum; 7ABR; -.
DR AlphaFoldDB; P37571; -.
DR SMR; P37571; -.
DR DIP; DIP-43708N; -.
DR IntAct; P37571; 5.
DR MINT; P37571; -.
DR STRING; 224308.BSU00860; -.
DR jPOST; P37571; -.
DR PaxDb; P37571; -.
DR PRIDE; P37571; -.
DR DNASU; 938481; -.
DR EnsemblBacteria; CAB11862; CAB11862; BSU_00860.
DR GeneID; 938481; -.
DR KEGG; bsu:BSU00860; -.
DR PATRIC; fig|224308.179.peg.87; -.
DR eggNOG; COG0542; Bacteria.
DR InParanoid; P37571; -.
DR OMA; SKMMQGE; -.
DR PhylomeDB; P37571; -.
DR BioCyc; BSUB:BSU00860-MON; -.
DR EvolutionaryTrace; P37571; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0030420; P:establishment of competence for transformation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1780.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR019489; Clp_ATPase_C.
DR InterPro; IPR036628; Clp_N_dom_sf.
DR InterPro; IPR004176; Clp_R_dom.
DR InterPro; IPR001270; ClpA/B.
DR InterPro; IPR018368; ClpA/B_CS1.
DR InterPro; IPR028299; ClpA/B_CS2.
DR InterPro; IPR041546; ClpA/ClpB_AAA_lid.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001943; UVR_dom.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF07724; AAA_2; 1.
DR Pfam; PF17871; AAA_lid_9; 1.
DR Pfam; PF02861; Clp_N; 2.
DR Pfam; PF10431; ClpB_D2-small; 1.
DR PRINTS; PR00300; CLPPROTEASEA.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01086; ClpB_D2-small; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF81923; SSF81923; 1.
DR PROSITE; PS51903; CLP_R; 1.
DR PROSITE; PS00870; CLPAB_1; 1.
DR PROSITE; PS00871; CLPAB_2; 1.
DR PROSITE; PS50151; UVR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Chaperone; Competence; Nucleotide-binding;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..810
FT /note="Negative regulator of genetic competence ClpC/MecB"
FT /id="PRO_0000191228"
FT DOMAIN 3..144
FT /note="Clp R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT DOMAIN 417..452
FT /note="UVR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00217"
FT REGION 6..71
FT /note="Repeat 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 80..144
FT /note="Repeat 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT REGION 163..410
FT /note="I"
FT REGION 471..662
FT /note="II"
FT BINDING 208..215
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 545..552
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 8..23
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 31..41
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 45..52
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 57..68
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 82..97
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 105..115
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 119..126
FT /evidence="ECO:0007829|PDB:2Y1Q"
FT HELIX 131..143
FT /evidence="ECO:0007829|PDB:2Y1Q"
SQ SEQUENCE 810 AA; 90119 MW; 9592231886C2E870 CRC64;
MMFGRFTERA QKVLALAQEE ALRLGHNNIG TEHILLGLVR EGEGIAAKAL QALGLGSEKI
QKEVESLIGR GQEMSQTIHY TPRAKKVIEL SMDEARKLGH SYVGTEHILL GLIREGEGVA
ARVLNNLGVS LNKARQQVLQ LLGSNETGSS AAGTNSNANT PTLDSLARDL TAIAKEDSLD
PVIGRSKEIQ RVIEVLSRRT KNNPVLIGEP GVGKTAIAEG LAQQIINNEV PEILRDKRVM
TLDMGTVVAG TKYRGEFEDR LKKVMDEIRQ AGNIILFIDE LHTLIGAGGA EGAIDASNIL
KPSLARGELQ CIGATTLDEY RKYIEKDAAL ERRFQPIQVD QPSVDESIQI LQGLRDRYEA
HHRVSITDDA IEAAVKLSDR YISDRFLPDK AIDLIDEAGS KVRLRSFTTP PNLKELEQKL
DEVRKEKDAA VQSQEFEKAA SLRDTEQRLR EQVEDTKKSW KEKQGQENSE VTVDDIAMVV
SSWTGVPVSK IAQTETDKLL NMENILHSRV IGQDEAVVAV AKAVRRARAG LKDPKRPIGS
FIFLGPTGVG KTELARALAE SIFGDEESMI RIDMSEYMEK HSTSRLVGSP PGYVGYDEGG
QLTEKVRRKP YSVVLLDEIE KAHPDVFNIL LQVLEDGRLT DSKGRTVDFR NTILIMTSNV
GASELKRNKY VGFNVQDETQ NHKDMKDKVM GELKRAFRPE FINRIDEIIV FHSLEKKHLT
EIVSLMSDQL TKRLKEQDLS IELTDAAKAK VAEEGVDLEY GARPLRRAIQ KHVEDRLSEE
LLRGNIHKGQ HIVLDVEDGE FVVKTTAKTN
