ID   2A5K_ARATH              Reviewed;         500 AA.
AC   Q93YV6;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Serine/threonine protein phosphatase 2A 57 kDa regulatory subunit B' kappa isoform;
DE            Short=AtB' kappa;
DE            Short=PP2A, B' subunit, kappa isoform;
GN   Name=B'KAPPA; OrderedLocusNames=At5g25510; ORFNames=T14C9.50;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   SUBCELLULAR LOCATION, AND INDUCTION BY EPIBRASSINOLIDE.
RX   PubMed=26517938; DOI=10.1016/j.molp.2015.10.007;
RA   Wang R., Liu M., Yuan M., Oses-Prieto J.A., Cai X., Sun Y.,
RA   Burlingame A.L., Wang Z.Y., Tang W.;
RT   "The brassinosteroid-activated BRI1 receptor kinase is switched off by
RT   dephosphorylation mediated by cytoplasm-localized PP2A B' subunits.";
RL   Mol. Plant 9:148-157(2016).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17368080; DOI=10.1016/j.tplants.2007.03.003;
RA   Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.;
RT   "Arabidopsis PPP family of serine/threonine phosphatases.";
RL   Trends Plant Sci. 12:169-176(2007).
CC   -!- FUNCTION: The B regulatory subunit may modulate substrate selectivity
CC       and catalytic activity, and also may direct the localization of the
CC       catalytic enzyme to a particular subcellular compartment.
CC       {ECO:0000250|UniProtKB:Q13362}.
CC   -!- SUBUNIT: PP2A consists of a common heteromeric enzyme, composed of a
CC       catalytic subunit (subunits C), a constant regulatory subunit (subunit
CC       A), and a variety of regulatory subunits such as subunits B (the
CC       R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families).
CC       {ECO:0000250|UniProtKB:Q13362}.
CC   -!- INTERACTION:
CC       Q93YV6; O23160: MYB73; NbExp=3; IntAct=EBI-25517060, EBI-25506855;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26517938}.
CC   -!- INDUCTION: Induced by epibrassinolide. {ECO:0000269|PubMed:26517938}.
CC   -!- SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B56
CC       family. {ECO:0000305}.
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DR   EMBL; AC006601; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED93456.1; -; Genomic_DNA.
DR   EMBL; AY059748; AAL24096.1; -; mRNA.
DR   EMBL; AY142557; AAN13126.1; -; mRNA.
DR   RefSeq; NP_197933.1; NM_122462.2.
DR   AlphaFoldDB; Q93YV6; -.
DR   SMR; Q93YV6; -.
DR   BioGRID; 17901; 4.
DR   IntAct; Q93YV6; 1.
DR   STRING; 3702.AT5G25510.1; -.
DR   iPTMnet; Q93YV6; -.
DR   PaxDb; Q93YV6; -.
DR   PRIDE; Q93YV6; -.
DR   ProteomicsDB; 245124; -.
DR   EnsemblPlants; AT5G25510.1; AT5G25510.1; AT5G25510.
DR   GeneID; 832626; -.
DR   Gramene; AT5G25510.1; AT5G25510.1; AT5G25510.
DR   KEGG; ath:AT5G25510; -.
DR   Araport; AT5G25510; -.
DR   TAIR; locus:2179499; AT5G25510.
DR   eggNOG; KOG2085; Eukaryota.
DR   HOGENOM; CLU_012437_4_1_1; -.
DR   OMA; VPLFCRI; -.
DR   OrthoDB; 890437at2759; -.
DR   PhylomeDB; Q93YV6; -.
DR   PRO; PR:Q93YV6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q93YV6; baseline and differential.
DR   Genevisible; Q93YV6; AT.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0000159; C:protein phosphatase type 2A complex; IEA:InterPro.
DR   GO; GO:0019888; F:protein phosphatase regulator activity; IEA:InterPro.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR002554; PP2A_B56.
DR   PANTHER; PTHR10257; PTHR10257; 1.
DR   Pfam; PF01603; B56; 1.
DR   PIRSF; PIRSF028043; PP2A_B56; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..500
FT                   /note="Serine/threonine protein phosphatase 2A 57 kDa
FT                   regulatory subunit B' kappa isoform"
FT                   /id="PRO_0000071468"
FT   REGION          1..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        19..53
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   500 AA;  56422 MW;  9CDCCDA0311BA9D1 CRC64;
     MFKQFLSKLP RKSSKSDSGE LNRSSSGPVS SPVQRSGTSG GGSGPVRSNS GKRMSSAVFP
     ASVVAGIEPL VPFKDVPSSE KLNLFVSKVS LCCVTFDFSD PGKNSIEKDV KRQTLLELLD
     FVASGSVKFT EPAILAMCRM CAVNLFRVFP PNYRSSSGGE NDDDEPMFDP AWPHLQIVYD
     LLLKFITSPC LDAKVAKKYL DHAFIVRLLD LFDSEDPRER ECLKTILHRV YGKFMVHRPF
     VRKSMSNIFY RFVFETEKHS GIAELLEIFG SIVSGFALPL KEEHKIFLWR VLIPLHKPKS
     VGNYFQQLSY CITQFIDKEP KLGSVVIKGL LKFWPITNSQ KEVMFLGEVE EIVEAMSVME
     FQKIMVPLFL RIACCVTSSH FQVSERALFL WNNDQIVNLI GHNRQAILPI MFTALEKNAQ
     NHWNQSVLNL TLNVRKMFCE MDEALFMSCH ARFKEDEAKQ CSAAEKRKEV WARLENAASM
     KPITGKTAVL VTPRATSIAC