ID   CLP_XANC5               Reviewed;         230 AA.
AC   Q3BYB3;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=CRP-like protein Clp;
DE   AltName: Full=Catabolite activation-like protein;
DE            Short=CAP-like;
GN   Name=clp; OrderedLocusNames=XCV0519;
OS   Xanthomonas campestris pv. vesicatoria (strain 85-10).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=316273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=85-10;
RX   PubMed=16237009; DOI=10.1128/jb.187.21.7254-7266.2005;
RA   Thieme F., Koebnik R., Bekel T., Berger C., Boch J., Buettner D.,
RA   Caldana C., Gaigalat L., Goesmann A., Kay S., Kirchner O., Lanz C.,
RA   Linke B., McHardy A.C., Meyer F., Mittenhuber G., Nies D.H.,
RA   Niesbach-Kloesgen U., Patschkowski T., Rueckert C., Rupp O., Schneiker S.,
RA   Schuster S.C., Vorhoelter F.J., Weber E., Puehler A., Bonas U., Bartels D.,
RA   Kaiser O.;
RT   "Insights into genome plasticity and pathogenicity of the plant pathogenic
RT   Bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete
RT   genome sequence.";
RL   J. Bacteriol. 187:7254-7266(2005).
CC   -!- FUNCTION: Global transcriptional regulator that regulates virulence
CC       factors production by activating or repressing the expression of a
CC       large set of genes in diffusible signal factor (DSF) pathway.
CC       {ECO:0000250}.
CC   -!- ACTIVITY REGULATION: Allosterically inhibited by cyclic di-GMP (c-di-
CC       GMP), which binds to Clp and abolishes its ability to bind its target
CC       gene promoter. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: Binding of c-di-GMP appears to trigger the active Clp
CC       conformation into an open form or inactive state, hence abolishing its
CC       DNA-binding ability. {ECO:0000250}.
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DR   EMBL; AM039952; CAJ22150.1; -; Genomic_DNA.
DR   RefSeq; WP_005914205.1; NZ_CP017190.1.
DR   AlphaFoldDB; Q3BYB3; -.
DR   SMR; Q3BYB3; -.
DR   STRING; 456327.BJD11_20280; -.
DR   EnsemblBacteria; CAJ22150; CAJ22150; XCV0519.
DR   GeneID; 63989762; -.
DR   GeneID; 66909689; -.
DR   KEGG; xcv:XCV0519; -.
DR   eggNOG; COG0664; Bacteria.
DR   HOGENOM; CLU_075053_3_5_6; -.
DR   OMA; FLEYCHI; -.
DR   Proteomes; UP000007069; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003824; F:catalytic activity; IEA:UniProtKB-KW.
DR   GO; GO:0035438; F:cyclic-di-GMP binding; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0046983; F:protein dimerization activity; ISS:UniProtKB.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   CDD; cd00038; CAP_ED; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR012318; HTH_CRP.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF00325; Crp; 1.
DR   PRINTS; PR00034; HTHCRP.
DR   SMART; SM00100; cNMP; 1.
DR   SMART; SM00419; HTH_CRP; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS00042; HTH_CRP_1; 1.
DR   PROSITE; PS51063; HTH_CRP_2; 1.
PE   3: Inferred from homology;
KW   Activator; Allosteric enzyme; c-di-GMP; Cytoplasm; DNA-binding; Repressor;
KW   Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..230
FT                   /note="CRP-like protein Clp"
FT                   /id="PRO_0000405704"
FT   DOMAIN          158..230
FT                   /note="HTH crp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   DNA_BIND        190..209
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   BINDING         18..139
FT                   /ligand="a nucleoside 3',5'-cyclic phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58464"
SQ   SEQUENCE   230 AA;  25621 MW;  6175A9AB2D4C1B30 CRC64;
     MSPGNTTVVT TTVRNATPSL ALDAGTIERF LAHSHRRRYP TRTDVFRPGD PAGTLYYVIS
     GSVSIIAEED DDRELVLGYF GSGEFVGEMG LFIESDTREV ILRTRTQCEL AEISYERLQQ
     LFQTSLSPDA PKILYAIGVQ LSKRLLDTTR KASRLAFLDV TDRIVRTLHD LAKEPEAMSH
     PQGTQLRVSR QELARLVGCS REMAGRVLKK LQADGLLHAR GKTVVLYGTR