CLR1_SCHPO
ID CLR1_SCHPO Reviewed; 1238 AA.
AC O74808;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Cryptic loci regulator protein 1;
GN Name=clr1; ORFNames=SPBC2D10.17;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RX PubMed=1644273; DOI=10.1093/genetics/131.2.287;
RA Thon G., Klar A.J.S.;
RT "The clr1 locus regulates the expression of the cryptic mating-type loci of
RT fission yeast.";
RL Genetics 131:287-296(1992).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP FUNCTION, INTERACTION WITH CLR3, AND SUBCELLULAR LOCATION.
RX PubMed=17289569; DOI=10.1016/j.cell.2006.12.035;
RA Sugiyama T., Cam H.P., Sugiyama R., Noma K., Zofall M., Kobayashi R.,
RA Grewal S.I.S.;
RT "SHREC, an effector complex for heterochromatic transcriptional
RT silencing.";
RL Cell 128:491-504(2007).
CC -!- FUNCTION: Regulates silencing of the mat2 and mat3 loci. Organizes the
CC chromatin structure of the mating-type region where it also
CC participates in establishing the 'cold spot' for recombination.
CC Required for proper positioning of nucleosomes at heterochromatic loci
CC and for transcriptional gene silencing (TGS) function of the Snf2/Hdac-
CC containing repressor complex (SHREC). {ECO:0000269|PubMed:1644273,
CC ECO:0000269|PubMed:17289569}.
CC -!- SUBUNIT: Interacts with clr3. {ECO:0000269|PubMed:17289569}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere. Chromosome,
CC telomere. Note=Associates with major heterochromatin, centromeres, sub-
CC telomeres, rDNA and the mat locus.
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DR EMBL; CU329671; CAA21175.1; -; Genomic_DNA.
DR PIR; T40120; T40120.
DR RefSeq; NP_596236.1; NM_001022156.2.
DR PDB; 5IKF; X-ray; 2.80 A; B=357-500.
DR PDB; 5IKJ; X-ray; 2.30 A; B=1151-1238.
DR PDBsum; 5IKF; -.
DR PDBsum; 5IKJ; -.
DR AlphaFoldDB; O74808; -.
DR SMR; O74808; -.
DR BioGRID; 277085; 107.
DR STRING; 4896.SPBC2D10.17.1; -.
DR iPTMnet; O74808; -.
DR MaxQB; O74808; -.
DR PaxDb; O74808; -.
DR PRIDE; O74808; -.
DR EnsemblFungi; SPBC2D10.17.1; SPBC2D10.17.1:pep; SPBC2D10.17.
DR GeneID; 2540558; -.
DR KEGG; spo:SPBC2D10.17; -.
DR PomBase; SPBC2D10.17; clr1.
DR VEuPathDB; FungiDB:SPBC2D10.17; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_260175_0_0_1; -.
DR InParanoid; O74808; -.
DR OMA; LKHRRFN; -.
DR PRO; PR:O74808; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0099115; C:chromosome, subtelomeric region; IDA:PomBase.
DR GO; GO:0031934; C:mating-type region heterochromatin; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005721; C:pericentric heterochromatin; IDA:PomBase.
DR GO; GO:0033553; C:rDNA heterochromatin; IDA:PomBase.
DR GO; GO:0070824; C:SHREC complex; IDA:PomBase.
DR GO; GO:0110129; C:SHREC2 complex; IDA:PomBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006325; P:chromatin organization; IMP:PomBase.
DR GO; GO:0031507; P:heterochromatin assembly; IMP:PomBase.
DR GO; GO:0030466; P:silent mating-type cassette heterochromatin assembly; IMP:PomBase.
DR InterPro; IPR013087; Znf_C2H2_type.
DR SMART; SM00355; ZnF_C2H2; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromatin regulator; Chromosome; Metal-binding;
KW Nucleus; Reference proteome; Telomere; Zinc; Zinc-finger.
FT CHAIN 1..1238
FT /note="Cryptic loci regulator protein 1"
FT /id="PRO_0000290637"
FT ZN_FING 1062..1087
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 133..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 196..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 277..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 546..568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 696..735
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 784..824
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 696..722
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT TURN 361..363
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 364..372
FT /evidence="ECO:0007829|PDB:5IKF"
FT STRAND 377..379
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 387..395
FT /evidence="ECO:0007829|PDB:5IKF"
FT TURN 396..399
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 404..408
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 411..413
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 417..426
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 432..434
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 447..464
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 469..478
FT /evidence="ECO:0007829|PDB:5IKF"
FT STRAND 485..490
FT /evidence="ECO:0007829|PDB:5IKF"
FT HELIX 1163..1173
FT /evidence="ECO:0007829|PDB:5IKJ"
FT STRAND 1212..1215
FT /evidence="ECO:0007829|PDB:5IKJ"
FT HELIX 1221..1226
FT /evidence="ECO:0007829|PDB:5IKJ"
FT STRAND 1227..1231
FT /evidence="ECO:0007829|PDB:5IKJ"
FT STRAND 1234..1238
FT /evidence="ECO:0007829|PDB:5IKJ"
SQ SEQUENCE 1238 AA; 136677 MW; 5C6F2213478758CC CRC64;
MAEPDISSSE TLTELQQLRL LYFFCFYHAA PFNVKTLVHS LIPPGALSYL LSTYDILRPW
LMALRVREGP VNDISTIVQL YEEIVKTGFF INPPPFESYS QTLVARITTL GRPKLQVQQE
AQSEVYQRAS TNTQQQVSNV SHGNFKPNSS VNTEPNTSIL SNSKYAGIKP FDFQSSSQNP
GLVCEQKTFY QHQFRPFSNL PSNKSSPVKH VSPNVKNNSK KTASSVNSNH SSIPSSITKS
NISSLDVYGS EKLISSGSQQ PGHGMVQTTS DKVNASASLY DRSPSKKDIT SSRNTSSYNL
GSMRNPSTLK NAAHANPFEG LRFQGSSAVL KEGLNSTVKK TFFDNLNSEK VCPSVSPFLT
PDNIASSILY STASFSRSKP DRPRLNLSLE LKLMQNELNK GQLKKQFKGD LRNLADWNNL
SLVSSKFPSL PITNLRPDGS FLKHRRFNEE IAYNRQTLEK AIKQLDLSPD KVIQLREQNG
VAVNGRVCYP TRNKHSEISA QSSSSLGVTK SLASEVYSSS TVDTISKLNT DKDNYLIKSK
KEPIQQKSVS SETTLVKPSS TSSYIDTTNN VLKTNSSFKS SGLTSGPRNE KELLPEGIPT
SHNNSETQAQ TADVSNIAAS ADGIYNSDQE KPPEKLDVTK RAFGREIENS NEKELLTSTF
LSPSAESQVC LAEIKTIRPG LVPKKQFSVD QNNVISDNTD CSLPKPSNSK LSSISSDGDA
SSNRMAVPDK SPFVHAAPNS KALTKDSFST HISVSSLLHS DNEISPIDST RKDYFTSKDS
NLQTLKEDAS STKQAKDSGT NDFDKLISGN DVSKNNSGEE QSRSALKPLI SGKLSSCESI
NLTKDISTVK RKEYFGIEST SSKQPFHDTG SIKIPAKRSF DTIDKDFRSS NIPFADKIKE
DGGDKNVISS IHITTELPKS MPVEVPTNAG AQSDQSNVVD SESLNLRENI STSVADVSLS
QAGNEAVLSK KACKPLVLID PFEEKVLKAF NMLSKGYAEY RCQWEGCLAN LHSLENFIKH
VLLLHHPKSC SVVKCLWASC DMVLPSEEFE MHLRGHLNNI RLNCEVSNCK KCFSNYEDMF
KHLQHSHLPF KFTPESFIKI RNGNVKEEAR RTRNAYTQKS GEVECFMETC TPIAKPAPAN
WYPVPPPGFN SSLLSRLTQS NQSKDKIIAA LAKRNVYKSF AGLYDSKGKN DNTGYDFDSN
YARVGRHGSF ILPVSKSVPT PSLLIEGSIV QRKNIKIE
