ID   CLRA_PSECO              Reviewed;          19 AA.
AC   P83448;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   11-DEC-2019, entry version 36.
DE   RecName: Full=Chlorate reductase subunit alpha;
DE            EC=1.97.1.1;
DE   Flags: Fragment;
OS   Pseudomonas chloritidismutans.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=203192;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=12730181; DOI=10.1128/jb.185.10.3210-3213.2003;
RA   Wolterink A.F.W.M., Schiltz E., Hagedoorn P.-L., Hagen W.R., Kengen S.W.M.,
RA   Stams A.J.M.;
RT   "Characterization of the chlorate reductase from Pseudomonas
RT   chloritidismutans.";
RL   J. Bacteriol. 185:3210-3213(2003).
CC   -!- FUNCTION: Reduces chlorate and bromate. {ECO:0000269|PubMed:12730181}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + chlorate = A + chlorite + H2O; Xref=Rhea:RHEA:16349,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:17441,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:49709; EC=1.97.1.1;
CC         Evidence={ECO:0000269|PubMed:12730181};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mo-bis(molybdopterin guanine dinucleotide);
CC         Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000305|PubMed:12730181};
CC       Note=Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-
CC       bis-MGD) cofactor per subunit. {ECO:0000305|PubMed:12730181};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=159 uM for chlorate;
CC         Vmax=51 umol/min/mg enzyme;
CC       pH dependence:
CC         Optimum pH is 7.5.;
CC   -!- SUBUNIT: Heterotrimer of alpha, beta and gamma subunits.
CC       {ECO:0000269|PubMed:12730181}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12730181}.
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DR   BRENDA; 1.97.1.1; 7803.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0047143; F:chlorate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   4Fe-4S; Cytoplasm; Direct protein sequencing; Iron; Iron-sulfur;
KW   Metal-binding; Molybdenum; Oxidoreductase.
FT   CHAIN           1..>19
FT                   /note="Chlorate reductase subunit alpha"
FT                   /id="PRO_0000089870"
FT   NON_TER         19
SQ   SEQUENCE   19 AA;  2166 MW;  FC9B39CBE29040F2 CRC64;
     LNMLEPVGET LASEYPYXK