ID   CLSB_BACSU              Reviewed;         398 AA.
AC   P45865;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Minor cardiolipin synthase ClsB;
GN   Name=clsB; Synonyms=ywjE; OrderedLocusNames=BSU37190;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA   Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA   Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA   Kunst F., Danchin A., Glaser P.;
RT   "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT   degrees).";
RL   Microbiology 143:3313-3328(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION IN THE CARDIOLIPIN BIOSYNTHESIS, DISRUPTION PHENOTYPE, AND
RP   NOMENCLATURE.
RX   PubMed=14973018; DOI=10.1128/jb.186.5.1475-1483.2004;
RA   Kawai F., Shoda M., Harashima R., Sadaie Y., Hara H., Matsumoto K.;
RT   "Cardiolipin domains in Bacillus subtilis marburg membranes.";
RL   J. Bacteriol. 186:1475-1483(2004).
CC   -!- FUNCTION: Involved in the biosynthesis of cardiolipin.
CC       {ECO:0000269|PubMed:14973018}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene show a lack of
CC       cardiolipin. {ECO:0000269|PubMed:14973018}.
CC   -!- SIMILARITY: Belongs to the phospholipase D family. Cardiolipin synthase
CC       subfamily. {ECO:0000305}.
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DR   EMBL; Z49782; CAA89866.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15747.1; -; Genomic_DNA.
DR   PIR; S55419; S55419.
DR   RefSeq; NP_391600.1; NC_000964.3.
DR   RefSeq; WP_003227602.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P45865; -.
DR   SMR; P45865; -.
DR   STRING; 224308.BSU37190; -.
DR   PaxDb; P45865; -.
DR   PRIDE; P45865; -.
DR   DNASU; 938460; -.
DR   EnsemblBacteria; CAB15747; CAB15747; BSU_37190.
DR   GeneID; 938460; -.
DR   KEGG; bsu:BSU37190; -.
DR   PATRIC; fig|224308.179.peg.4029; -.
DR   eggNOG; COG1502; Bacteria.
DR   InParanoid; P45865; -.
DR   OMA; YEYFMQY; -.
DR   PhylomeDB; P45865; -.
DR   BioCyc; BSUB:BSU37190-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008808; F:cardiolipin synthase activity; IMP:UniProtKB.
DR   GO; GO:0032049; P:cardiolipin biosynthetic process; IMP:UniProtKB.
DR   GO; GO:0043934; P:sporulation; IMP:UniProtKB.
DR   InterPro; IPR022924; Cardiolipin_synthase.
DR   InterPro; IPR025202; PLD-like_dom.
DR   InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR   Pfam; PF13091; PLDc_2; 2.
DR   SMART; SM00155; PLDc; 2.
DR   TIGRFAMs; TIGR04265; bac_cardiolipin; 1.
DR   PROSITE; PS50035; PLD; 2.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..398
FT                   /note="Minor cardiolipin synthase ClsB"
FT                   /id="PRO_0000201281"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          141..168
FT                   /note="PLD phosphodiesterase 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
FT   DOMAIN          311..338
FT                   /note="PLD phosphodiesterase 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
SQ   SEQUENCE   398 AA;  45842 MW;  4E233A4B97727756 CRC64;
     MKVFIVIMII VVIFFALILL DIFMGRAGYR KKAYEPVFSK KKSDIELIHC GADLVERMMN
     DIRQAASSVH MMFFIMKNDE VSHNMYTLLK TKAQAGVSVY LLLDWAGCRA IKKTALQTMK
     NAGVHVHVMN RPRFPFFFFH MQKRNHRKIT VIDGKIGYIG GFNIAEEYLG KKAKFGNWED
     YHLRMIGEGV HDLQTLFASD LKRNTGIELG SDVWPKLQQG TISHKIYATD GYSLENIYLA
     NIAQAKNRLT VCTPYYIPSK PLQEALINAR KNGVSVRIIV PMKSDHPLVR EAAFTYYSEL
     LDAGCLIYRY YQGFYHVKAL IIDDHLSIIG TANFDKRSLF LNEEVNVEID DEAFTSEVYA
     TIEEDMKKSE LLTMEDFSKR TFRQRPAEWL GRALSYFL