CLSPN_DROME
ID CLSPN_DROME Reviewed; 1465 AA.
AC Q8IRB5; Q8MYR6; X2JAN5;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Claspin {ECO:0000312|FlyBase:FBgn0052251};
GN Name=Claspin {ECO:0000312|FlyBase:FBgn0052251};
GN ORFNames=CG32251 {ECO:0000312|FlyBase:FBgn0052251};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 761-1465.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350 AND SER-963, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-49; SER-52; SER-64;
RP SER-75; SER-109; SER-114; SER-350; SER-354; SER-381; SER-404; SER-406;
RP SER-432; SER-434; SER-444; SER-456; SER-458; SER-468; THR-693; SER-963;
RP THR-973; SER-990; SER-1093; SER-1094 AND SER-1287, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, AND PHOSPHORYLATION.
RX PubMed=22796626; DOI=10.1016/j.dnarep.2012.06.007;
RA Lee E.M., Trinh T.T., Shim H.J., Park S.Y., Nguyen T.T., Kim M.J.,
RA Song Y.H.;
RT "Drosophila Claspin is required for the G2 arrest that is induced by DNA
RT replication stress but not by DNA double-strand breaks.";
RL DNA Repair 11:741-752(2012).
CC -!- FUNCTION: Required for checkpoint signaling in response to DNA
CC replication stress; either resulting from normal embryogenesis or
CC induced by the DNA synthesis inhibitor hydroxyurea (HU). It is not
CC required for the G2 arrest resulting from DNA double strand breaks
CC induced by ionizing irradiation (IR). Necessary for the timely
CC phosphorylation of Cdk1 at the mid-blastula transition. May have a
CC minor role in maintaining genomic stability in mitotic cells.
CC {ECO:0000269|PubMed:22796626}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22796626}.
CC -!- TISSUE SPECIFICITY: Detected in the ovary but not in the testis (at
CC protein level). {ECO:0000269|PubMed:22796626}.
CC -!- DEVELOPMENTAL STAGE: Expressed during S phase, in a cell-cycle-
CC dependent fashion. Expression levels are highest in embryos (0-4 hours
CC old) and in the ovaries of adult females. Little to no expression in
CC larvae and pupae (at protein level). {ECO:0000269|PubMed:22796626}.
CC -!- PTM: Phosphorylated in response to DNA damage by IR and HU treatment.
CC Phosphorylation does not require mei-41 or tefu.
CC {ECO:0000269|PubMed:22796626}.
CC -!- DISRUPTION PHENOTYPE: Hatch rate of embryos is less than one percent.
CC Nuclear morphology of embryos is normal until the 10th nuclear cycle
CC when the shape and size of nuclei becomes abnormal and surface nuclei
CC also begin to exhibit an irregular distribution. The phosphorylation of
CC Cdk1 at the mid-blastula transition (between 2 to 4 hours after egg
CC deposition) is also severely delayed. Increased sensitivity to HU with
CC reduced survival rates. In the eye disks from mutant third stage larvae
CC treated with HU, there is a higher percentage of mitotic cells compared
CC to untreated mutant larvae. Is insensitive to ionizing radiation; the
CC percentage of mitotic cells is unaffected in the eye disks of larvae
CC treated with IR, and IR-induced apoptosis in larvae also appears to be
CC unaffected. {ECO:0000269|PubMed:22796626}.
CC -!- SIMILARITY: Belongs to the claspin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM29654.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAN11599.1; -; Genomic_DNA.
DR EMBL; AE014296; AHN57959.1; -; Genomic_DNA.
DR EMBL; AY113649; AAM29654.1; ALT_INIT; mRNA.
DR RefSeq; NP_001286934.1; NM_001300005.1.
DR RefSeq; NP_728981.1; NM_168080.2.
DR AlphaFoldDB; Q8IRB5; -.
DR BioGRID; 77458; 5.
DR IntAct; Q8IRB5; 4.
DR STRING; 7227.FBpp0073142; -.
DR iPTMnet; Q8IRB5; -.
DR PaxDb; Q8IRB5; -.
DR PRIDE; Q8IRB5; -.
DR EnsemblMetazoa; FBtr0073286; FBpp0073142; FBgn0052251.
DR EnsemblMetazoa; FBtr0346538; FBpp0312156; FBgn0052251.
DR GeneID; 326205; -.
DR KEGG; dme:Dmel_CG32251; -.
DR UCSC; CG32251-RA; d. melanogaster.
DR CTD; 326205; -.
DR FlyBase; FBgn0052251; Claspin.
DR VEuPathDB; VectorBase:FBgn0052251; -.
DR eggNOG; KOG4156; Eukaryota.
DR GeneTree; ENSGT00390000012738; -.
DR HOGENOM; CLU_265940_0_0_1; -.
DR InParanoid; Q8IRB5; -.
DR OMA; KGDANMV; -.
DR OrthoDB; 201318at2759; -.
DR PhylomeDB; Q8IRB5; -.
DR Reactome; R-DME-111465; Apoptotic cleavage of cellular proteins.
DR Reactome; R-DME-176187; Activation of ATR in response to replication stress.
DR Reactome; R-DME-5693607; Processing of DNA double-strand break ends.
DR SignaLink; Q8IRB5; -.
DR BioGRID-ORCS; 326205; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 326205; -.
DR PRO; PR:Q8IRB5; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0052251; Expressed in ovary and 17 other tissues.
DR Genevisible; Q8IRB5; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0010997; F:anaphase-promoting complex binding; IBA:GO_Central.
DR GO; GO:0032147; P:activation of protein kinase activity; IBA:GO_Central.
DR GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IMP:FlyBase.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IBA:GO_Central.
DR InterPro; IPR024146; Claspin.
DR PANTHER; PTHR14396; PTHR14396; 2.
PE 1: Evidence at protein level;
KW Cell cycle; Coiled coil; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..1465
FT /note="Claspin"
FT /id="PRO_0000372855"
FT REGION 1..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 544..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 585..636
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 845..977
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1058..1154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 260..281
FT /evidence="ECO:0000255"
FT COILED 564..587
FT /evidence="ECO:0000255"
FT COILED 839..874
FT /evidence="ECO:0000255"
FT COMPBIAS 22..36
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..78
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..157
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..421
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..445
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 591..606
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 607..631
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 858..877
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 878..893
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 894..940
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 942..960
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1061..1080
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1094..1111
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1129..1154
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 45
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 49
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 52
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 64
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 109
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 350
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656,
FT ECO:0000269|PubMed:18327897"
FT MOD_RES 354
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 381
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 404
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 406
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 432
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 434
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 444
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 456
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 458
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 468
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 693
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 963
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656,
FT ECO:0000269|PubMed:18327897"
FT MOD_RES 973
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 990
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1093
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1094
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1287
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 1083..1084
FT /note="VP -> ET (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
FT CONFLICT 1114
FT /note="T -> I (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
FT CONFLICT 1140
FT /note="D -> E (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
FT CONFLICT 1240
FT /note="E -> D (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
FT CONFLICT 1378
FT /note="I -> V (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
FT CONFLICT 1385
FT /note="S -> T (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
FT CONFLICT 1447
FT /note="I -> M (in Ref. 3; AAM29654)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1465 AA; 165283 MW; D6072ABB499597F0 CRC64;
MSESLAETAA AAENEEQADD SMEKLVFDEE DEQMVSGEDL LGGKSLIMSD DSEAEDGSQE
KPLSPKDRRR RMMDSDQEED AMDQGNGDSI KNSEDVEEEM VSRPKKKVSA IIDSDNDDEQ
QAEKKTQKEE GKQPVKSKKK RSQVNQEDQE KPVKSNKTKK AVKNKTQANK AEDDQDNPSK
EDKPKKVVKN KKQTNKDGKP QTNEEYDHHQ QHEKPAKTQK SKKLAKKQKQ QEDDKEDNGT
EQEKKKPSPK SKAKKSDKSK IDSLMDNEED AGEDLKMYQE DQEPQKQKTK KSAKKTNKDS
KEESGEDQEH QEQKKPLKKI KLDNIDTKED KEQIVKPKKM AKKNKQKVLS DDESEENQNE
KVDQDHDLKK MSSENELEMG SDKEDQEMQE PPKKAKKNKQ RMDSESEDEI PKTESEKITS
SPKNKLKGLV DSESEPEETA EEVSPVKNKL KGLVDSESEP ELDNPEESAG EQEAPMESAL
SREKPKKAKV VRESAKKALE GMQAIQSEQQ RLHREAHINV PYHQPKPRTL KEFLSRRTIN
APLATALAGG SPMPSRQPRK SVGLRMTREE LEAYAKLMED RAKEATEFFK SESESDEEDD
SENEEPMELK DNPGVMDEML DNPKTPEQPK NDTEDMTSQA VIEVPVLGED KTDDTVGDEA
MVADSITEEE PIASTSTAAA LKLADNFEIQ QETNAKSPSK VCLVTEVVEL PKLDLSTINI
TPSSKPATPK ISEVIRQLKI EKSLDESPSL KGDPNMVIDL ETGDMFAKKP TGVDDLLNRL
MKTREAKKHK TTETVNILTT EHGKLEMSKV SIHLHEEEIA KEPKPGAGYM KMQEHLKTLI
TKKRMEDLRK KQAEEQEKMA EDEEEGMDVD EEYEPEDKPG YAEVTINEDE EIIDKVDSTA
EDDGDAEENK NDADEDPEDN PVEDSEDEKN DSESEQECEA NPEPETQTRK KNRIIKAFED
DNSDEDDLDL LQTPKPSNVA PITATQLQLS AHKLFDVETR RTASDEENEL LDLCSGQFPQ
TQMLSSDAPS VDTAVISQIP MTQFGGSSQA ADELEGLCSG TFATQLPSQA PTQQPEQQEE
SEVPAAVANR IVSSDEEAQE DALEVDKPRN KKLTKKRPKK KAKLGFSDDE DSDDEVEEFD
EESDAEPVEE IPETFVDYDS EENEIVVEMT KKDRKIRAAN FVDKEAELSE SEWGSADEDE
KNMDEYDIEL GDEDQFDRDK LRHELGQIHA RKMMDQDIRE VRKIKELLFE EEEEGANRQR
QFRWKNVEAG FSLDDNRTDN GEGNDGSGDE ENEHLWRKIR YEREQLLLEK GLKPEVASPL
STSVINTSNN GNSPAIRRLN IISSKKTTVE VKKNSPFLIS KTIAGKQQKS AVRGSFLIRD
QQTLSKLAGL TKGTSGDVDA AEGTISVKSA KAKNFVFATL TEEEHENRKR KAADILNSST
ETGVNFIKKP KLEPRRDKCL IDQLL
