CLT1_ARATH
ID CLT1_ARATH Reviewed; 447 AA.
AC Q058P6;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Protein CLT1, chloroplastic {ECO:0000303|PubMed:20080670};
DE AltName: Full=CRT-like transporter 1 {ECO:0000303|PubMed:20080670};
DE AltName: Full=Chloroquine-resistance transporter-like transporter 1 {ECO:0000303|PubMed:20080670};
DE Flags: Precursor;
GN Name=CLT1 {ECO:0000303|PubMed:20080670};
GN OrderedLocusNames=At5g19380 {ECO:0000312|Araport:AT5G19380};
GN ORFNames=F7K24.130 {ECO:0000312|EMBL:AF296837};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABJ17107.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clone.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=20080670; DOI=10.1073/pnas.0913689107;
RA Maughan S.C., Pasternak M., Cairns N., Kiddle G., Brach T., Jarvis R.,
RA Haas F., Nieuwland J., Lim B., Muller C., Salcedo-Sora E., Kruse C.,
RA Orsel M., Hell R., Miller A.J., Bray P., Foyer C.H., Murray J.A.,
RA Meyer A.J., Cobbett C.S.;
RT "Plant homologs of the Plasmodium falciparum chloroquine-resistance
RT transporter, PfCRT, are required for glutathione homeostasis and stress
RT responses.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:2331-2336(2010).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=24204368; DOI=10.3389/fpls.2013.00416;
RA Schnaubelt D., Schulz P., Hannah M.A., Yocgo R.E., Foyer C.H.;
RT "A phenomics approach to the analysis of the influence of glutathione on
RT leaf area and abiotic stress tolerance in Arabidopsis thaliana.";
RL Front. Plant Sci. 4:416-416(2013).
CC -!- FUNCTION: Involved in thiol transport from the plastid to the cytosol.
CC Transports probably both glutathione (GSH) and its precursor, gamma-
CC glutamylcysteine (gamma-EC). Exhibits some functional redundancy with
CC CLT3 in maintaining the root GSH pool. {ECO:0000269|PubMed:20080670}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:20080670}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. {ECO:0000305};
CC Name=1;
CC IsoId=Q058P6-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, but resistance to L-
CC buthionine-SR-sulfoximine (BSO), an inhibitor of GSH1. Clt1, clt3 and
CC clt3 triple mutants are more sensitive to Cd(2+), have a decreased
CC level of cytosolic GSH, an altered systemic acquired resistance
CC response and are more sensitive to Phytophthora infection
CC (PubMed:20080670). Clt1, clt3 and clt3 triple mutants have decreased
CC lateral root densities (PubMed:24204368). {ECO:0000269|PubMed:20080670,
CC ECO:0000269|PubMed:24204368}.
CC -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF296837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92693.1; -; Genomic_DNA.
DR EMBL; BT029172; ABJ17107.1; -; mRNA.
DR RefSeq; NP_568373.1; NM_121943.3. [Q058P6-1]
DR AlphaFoldDB; Q058P6; -.
DR SMR; Q058P6; -.
DR TCDB; 2.A.7.20.4; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q058P6; -.
DR PRIDE; Q058P6; -.
DR ProteomicsDB; 240984; -. [Q058P6-1]
DR EnsemblPlants; AT5G19380.1; AT5G19380.1; AT5G19380. [Q058P6-1]
DR GeneID; 832058; -.
DR Gramene; AT5G19380.1; AT5G19380.1; AT5G19380. [Q058P6-1]
DR KEGG; ath:AT5G19380; -.
DR Araport; AT5G19380; -.
DR eggNOG; ENOG502QR5M; Eukaryota.
DR OMA; ITYDYTE; -.
DR PhylomeDB; Q058P6; -.
DR PRO; PR:Q058P6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q058P6; baseline and differential.
DR Genevisible; Q058P6; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR013936; CRT-like.
DR PANTHER; PTHR31326; PTHR31326; 1.
DR Pfam; PF08627; CRT-like; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..447
FT /note="Protein CLT1, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000433246"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 447 AA; 47925 MW; B1813E5C84AFE3E3 CRC64;
MATTSSDRLI AGLTASIGSI ESRYANPAQS VSLICRNQIN GAPPIVLRSS RRSRLWLIEA
IPPAKSWNGS NDGDEDIKKS DTRNYAIGGT GGHAVAGKDD RTMEIVIAAA TTAALGVGNR
VLYKLALIPL KQYPFFLAQL STFGYVAVYF SILYFRYRAG IVTKEMLSVP KLPFLIVGVL
ESLALAAGMA AASNLSGPST TVLSQTFLVW QILFSIIFLG RRYRINQILG CTLVAFGVIV
SVASGSGAAH SFKDTGILWS LLMVFSFLLQ GADTVMKEVI FLDSKKRLKG ASLDLFVVNS
YGSIFQVICI ALLLPFLSKL WGIPFNQLPS YIRDGGACFL NIGSRITGCE GAPLLPVMFV
MMNMAYNISL LRLIKISSAV VSSLASTVSV PIAVYCFTLP LPYLGVASTL PRGFVAGTII
LVVGMLLYAW TPSTNTSDSI IPSPPST
