CLU5D_COLGM
ID CLU5D_COLGM Reviewed; 478 AA.
AC E3QFA7;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 1.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=Pathogenicity cluster 5 protein d {ECO:0000303|PubMed:31599055};
DE Flags: Precursor;
GN Name=CLU5d {ECO:0000303|PubMed:31599055}; ORFNames=GLRG_04689;
OS Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize
OS anthracnose fungus) (Glomerella graminicola).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC Colletotrichum graminicola species complex.
OX NCBI_TaxID=645133;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=M1.001 / M2 / FGSC 10212;
RX PubMed=22885923; DOI=10.1038/ng.2372;
RA O'Connell R.J., Thon M.R., Hacquard S., Amyotte S.G., Kleemann J.,
RA Torres M.F., Damm U., Buiate E.A., Epstein L., Alkan N., Altmueller J.,
RA Alvarado-Balderrama L., Bauser C.A., Becker C., Birren B.W., Chen Z.,
RA Choi J., Crouch J.A., Duvick J.P., Farman M.A., Gan P., Heiman D.,
RA Henrissat B., Howard R.J., Kabbage M., Koch C., Kracher B., Kubo Y.,
RA Law A.D., Lebrun M.-H., Lee Y.-H., Miyara I., Moore N., Neumann U.,
RA Nordstroem K., Panaccione D.G., Panstruga R., Place M., Proctor R.H.,
RA Prusky D., Rech G., Reinhardt R., Rollins J.A., Rounsley S., Schardl C.L.,
RA Schwartz D.C., Shenoy N., Shirasu K., Sikhakolli U.R., Stueber K.,
RA Sukno S.A., Sweigard J.A., Takano Y., Takahara H., Trail F.,
RA van der Does H.C., Voll L.M., Will I., Young S., Zeng Q., Zhang J.,
RA Zhou S., Dickman M.B., Schulze-Lefert P., Ver Loren van Themaat E.,
RA Ma L.-J., Vaillancourt L.J.;
RT "Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered
RT by genome and transcriptome analyses.";
RL Nat. Genet. 44:1060-1065(2012).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=18785828; DOI=10.1094/mpmi-21-10-1325;
RA Krijger J.J., Horbach R., Behr M., Schweizer P., Deising H.B., Wirsel S.G.;
RT "The yeast signal sequence trap identifies secreted proteins of the
RT hemibiotrophic corn pathogen Colletotrichum graminicola.";
RL Mol. Plant Microbe Interact. 21:1325-1336(2008).
RN [3]
RP INDUCTION.
RX PubMed=26956617; DOI=10.1186/s12864-016-2546-0;
RA Torres M.F., Ghaffari N., Buiate E.A., Moore N., Schwartz S., Johnson C.D.,
RA Vaillancourt L.J.;
RT "A Colletotrichum graminicola mutant deficient in the establishment of
RT biotrophy reveals early transcriptional events in the maize anthracnose
RT disease interaction.";
RL BMC Genomics 17:202-202(2016).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=31599055; DOI=10.1111/1462-2920.14819;
RA Eisermann I., Weihmann F., Krijger J.J., Kroeling C., Hause G., Menzel M.,
RA Pienkny S., Kiesow A., Deising H.B., Wirsel S.G.R.;
RT "Two genes in a pathogenicity gene cluster encoding secreted proteins are
RT required for appressorial penetration and infection of the maize
RT anthracnose fungus Colletotrichum graminicola.";
RL Environ. Microbiol. 21:4773-4791(2019).
CC -!- FUNCTION: Secreted protein required for appressorial penetration of
CC intact host epidermal cells and for pathogenicit, but not for
CC subsequent biotrophic and necrotrophic colonization of leaves.
CC {ECO:0000269|PubMed:31599055}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18785828,
CC ECO:0000269|PubMed:31599055}.
CC -!- INDUCTION: Expressed at all stages of pathogenesis (PubMed:22885923,
CC PubMed:26956617, PubMed:31599055). Among the most highly expressed
CC genes in appressoria and biotrophic hyphae in planta (PubMed:26956617).
CC {ECO:0000269|PubMed:22885923, ECO:0000269|PubMed:26956617,
CC ECO:0000269|PubMed:31599055}.
CC -!- DISRUPTION PHENOTYPE: Leads to virulence defects.
CC {ECO:0000269|PubMed:31599055}.
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DR EMBL; GG697345; EFQ29545.1; -; Genomic_DNA.
DR RefSeq; XP_008093565.1; XM_008095374.1.
DR AlphaFoldDB; E3QFA7; -.
DR EnsemblFungi; EFQ29545; EFQ29545; GLRG_04689.
DR GeneID; 24410054; -.
DR VEuPathDB; FungiDB:GLRG_04689; -.
DR eggNOG; ENOG502QU23; Eukaryota.
DR HOGENOM; CLU_029378_0_3_1; -.
DR OrthoDB; 1190857at2759; -.
DR Proteomes; UP000008782; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Glycoprotein; Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..478
FT /note="Pathogenicity cluster 5 protein d"
FT /id="PRO_5003180481"
FT REGION 35..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 328
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 332
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 478 AA; 47554 MW; EE1E652A0CF9647F CRC64;
MQIQNLIAAL AGMAVVAEAS VTFLDSPLSA ALAKRQNKGG NNNNNNNNNN NNNNNNNKNN
GGNNQLCLNP NNVQKGSQQA GTPKQGQANS AVDQANFINF CTGQQLTNGE QKTAGSCNGV
VMGKIPSQNN MVSTIIKNPP PGGNLQANQQ FNVQLKVNNL QAGSFTDAQS TYYSAPQDTN
GAGNIIGHVH VTIQDMGNSL TPQNALDPKQ FVFFKGIDDA GDGKGNLQAT VTGGLPAGNY
RVCTMSSASN HQPVLMPVAQ RGAQDDCNKF TVGGNGGNGG NGGNGGNGGN GGNGGNGGNG
GKNAAVNSGN ADNGGNNDGG GGGNDGGNNS ANNSGSGNKQ GGKQQNGAST GGFPGTSNQF
PGKTGTGSGA QTGNANTKTQ AGGSASNSAT NGNSGTGGKG GATANAIGGI QAPAVTNSGD
SSRPFAVNGN TFVNKAAAVQ RACDIQRNGC FDAINRGKLT GSTADCDAQL QTCTQQLS
