CLUA1_CAEEL
ID CLUA1_CAEEL Reviewed; 404 AA.
AC Q6I6D4; G5EEH8; Q6I6D5;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Clusterin-associated protein 1 homolog;
GN Name=dyf-3 {ECO:0000312|WormBase:C04C3.5a};
GN ORFNames=C04C3.5 {ECO:0000312|WormBase:C04C3.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3), TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15713455; DOI=10.1016/j.jmb.2004.12.005;
RA Murayama T., Toh Y., Ohshima Y., Koga M.;
RT "The dyf-3 gene encodes a novel protein required for sensory cilium
RT formation in Caenorhabditis elegans.";
RL J. Mol. Biol. 346:677-687(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, IDENTIFICATION IN IFT COMPLEX B, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=28479320; DOI=10.1016/j.cub.2017.04.015;
RA Yi P., Li W.J., Dong M.Q., Ou G.;
RT "Dynein-driven retrograde intraflagellar transport is triphasic in C.
RT elegans sensory cilia.";
RL Curr. Biol. 27:1448-1461(2017).
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B
CC required for transport of proteins in the motile cilium
CC (PubMed:28479320). May be required for ciliary entrance and transport
CC of specific ciliary cargo proteins such as che-3 which are related to
CC motility (PubMed:28479320). Required for sensory cilia biogenesis
CC (PubMed:15713455). {ECO:0000269|PubMed:15713455,
CC ECO:0000269|PubMed:28479320}.
CC -!- SUBUNIT: Component of the IFT complex B composed of at least che-2,
CC che-13, dyf-1, dyf-3, dyf-6, dyf-11, dyf-13, ift-20, ift-74, ift-81,
CC ifta-2, osm-1, osm-5 and osm-6. {ECO:0000269|PubMed:28479320}.
CC -!- SUBCELLULAR LOCATION: Cell projection, axon
CC {ECO:0000269|PubMed:15713455}. Cell projection, cilium
CC {ECO:0000269|PubMed:15713455}. Cell projection, dendrite
CC {ECO:0000269|PubMed:15713455}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=dyf-3a;
CC IsoId=Q6I6D4-1; Sequence=Displayed;
CC Name=2; Synonyms=dyf-3b;
CC IsoId=Q6I6D4-2; Sequence=VSP_046490;
CC Name=3; Synonyms=dyf-3c;
CC IsoId=Q6I6D4-3; Sequence=VSP_046489;
CC -!- TISSUE SPECIFICITY: Expressed in a subset of sensory neurons including
CC eight pairs of amphid neurons (ASE, ADF, ASG, ASH, ASI, ASJ, ASK and
CC ADL), six inner labial neurons (IL2s) and two pairs of phasmid neurons
CC (PHA and PHB). {ECO:0000269|PubMed:15713455}.
CC -!- DISRUPTION PHENOTYPE: Cilia of at least eight pairs of amphid neurons
CC from mutant dyf(m185) animals are truncated at a middle segment, and
CC empty amphidial sockets were seen indicating cilium structural
CC abnormalities. {ECO:0000269|PubMed:15713455}.
CC -!- SIMILARITY: Belongs to the CLUAP1 family. {ECO:0000305}.
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DR EMBL; AB182367; BAD23996.1; -; Genomic_DNA.
DR EMBL; AB182367; BAD23997.1; -; Genomic_DNA.
DR EMBL; AB182367; BAD23998.1; -; Genomic_DNA.
DR EMBL; FO080230; CCD62198.1; -; Genomic_DNA.
DR EMBL; FO080230; CCD62199.1; -; Genomic_DNA.
DR EMBL; FO080230; CCD62200.1; -; Genomic_DNA.
DR RefSeq; NP_001021294.1; NM_001026123.1.
DR RefSeq; NP_001021295.1; NM_001026124.2.
DR RefSeq; NP_001021296.1; NM_001026125.1. [Q6I6D4-2]
DR AlphaFoldDB; Q6I6D4; -.
DR SMR; Q6I6D4; -.
DR BioGRID; 42247; 7.
DR ComplexPortal; CPX-1290; Intraflagellar transport complex B.
DR IntAct; Q6I6D4; 5.
DR STRING; 6239.C04C3.5b; -.
DR PaxDb; Q6I6D4; -.
DR PeptideAtlas; Q6I6D4; -.
DR EnsemblMetazoa; C04C3.5a.1; C04C3.5a.1; WBGene00001119. [Q6I6D4-3]
DR EnsemblMetazoa; C04C3.5a.2; C04C3.5a.2; WBGene00001119. [Q6I6D4-3]
DR EnsemblMetazoa; C04C3.5b.1; C04C3.5b.1; WBGene00001119. [Q6I6D4-1]
DR EnsemblMetazoa; C04C3.5c.1; C04C3.5c.1; WBGene00001119. [Q6I6D4-2]
DR GeneID; 177107; -.
DR UCSC; C04C3.5b; c. elegans.
DR CTD; 177107; -.
DR WormBase; C04C3.5a; CE16811; WBGene00001119; dyf-3. [Q6I6D4-3]
DR WormBase; C04C3.5b; CE38264; WBGene00001119; dyf-3. [Q6I6D4-1]
DR WormBase; C04C3.5c; CE38265; WBGene00001119; dyf-3. [Q6I6D4-2]
DR eggNOG; KOG3647; Eukaryota.
DR GeneTree; ENSGT00390000008957; -.
DR HOGENOM; CLU_034981_1_0_1; -.
DR InParanoid; Q6I6D4; -.
DR OMA; RKVYGNM; -.
DR OrthoDB; 874966at2759; -.
DR PhylomeDB; Q6I6D4; -.
DR Reactome; R-CEL-5620924; Intraflagellar transport.
DR PRO; PR:Q6I6D4; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00001119; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0030424; C:axon; IDA:UniProtKB.
DR GO; GO:0042995; C:cell projection; IDA:UniProtKB.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0005929; C:cilium; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IDA:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; IDA:UniProtKB.
DR GO; GO:0007635; P:chemosensory behavior; IMP:UniProtKB.
DR GO; GO:0006935; P:chemotaxis; IMP:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; IDA:UniProtKB.
DR GO; GO:0042073; P:intraciliary transport; IC:ComplexPortal.
DR GO; GO:0010446; P:response to alkaline pH; IMP:UniProtKB.
DR InterPro; IPR019366; Clusterin-associated_protein-1.
DR PANTHER; PTHR21547; PTHR21547; 1.
DR Pfam; PF10234; Cluap1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Coiled coil; Reference proteome.
FT CHAIN 1..404
FT /note="Clusterin-associated protein 1 homolog"
FT /id="PRO_0000422195"
FT REGION 328..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 176..306
FT /evidence="ECO:0000255"
FT COMPBIAS 336..354
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..380
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..73
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_046489"
FT VAR_SEQ 1..11
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046490"
SQ SEQUENCE 404 AA; 47260 MW; 028AC418A2D01B14 CRC64;
MSYRELRNLC EMTRSLRYPR LMSIENFRTP NFQLVAELLE WIVKKFEPES NLDAHEVQTE
ADRVAFIKNA VLLMLQNSRI KMNPKKLYQA DGHAVQELLP AMKILYQAKA EDPNTDNSPK
WTQVKNKLSS KMQEVRITRQ LSSQLPETGA LLSELLSRQE FISQQHERAA SRAVPLAEAE
KVLQATVQNI AQETEQLSNK LNNVASDEAE LDEKIERKKR EYEQLQKRFA KLQSFRPQYM
DEYERFEERL KKLYEVYVLN FRNLSYLRKV HDDLARSERQ RQEELEKAMR MAVEKMRLEQ
EKKDAIGLHD DDGSDAPLVD RRQSVRKVYG NMMGGDASDD DEDDEDDEDD EIIRMDDNVA
QHHKRELHRQ EEDEERSEAD YMVGITTAHV ADDAKSDLSS GDDF
