ID   CLUA1_DANRE             Reviewed;         427 AA.
AC   Q7ZVC2; Q4V941; Q6NWG3;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Clusterin-associated protein 1 homolog;
DE   AltName: Full=Qilin {ECO:0000303|PubMed:22102889};
GN   Name=cluap1; ORFNames=zgc:56115 {ECO:0000303|Ref.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD; TISSUE=Kidney, and Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=22102889; DOI=10.1371/journal.pone.0027365;
RA   Li J., Sun Z.;
RT   "Qilin is essential for cilia assembly and normal kidney development in
RT   zebrafish.";
RL   PLoS ONE 6:E27365-E27365(2011).
CC   -!- FUNCTION: Required for cilia biogenesis and maintenance in the kidney,
CC       the lateral line organ and eye. Appears to function within the multiple
CC       intraflagellar transport complex B (IFT-B).
CC       {ECO:0000269|PubMed:22102889}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96AJ1}. Cell
CC       projection, cilium {ECO:0000305|PubMed:22102889}.
CC   -!- DEVELOPMENTAL STAGE: Expressed maternally and ubiquitously throughout
CC       the embryo across different developmental stages from early 8-cell
CC       stage to 24 hpf. {ECO:0000269|PubMed:22102889}.
CC   -!- DISRUPTION PHENOTYPE: Mutant animals develop ventrally curved bodies
CC       visible at the end of 1 dpf, followed by bilateral kidney cysts in the
CC       glomerular-tubular region detectable at 2 dpf. Cilia in the anterior
CC       pronephric ducts are already visibly defective at 1 dpf and by 5 dpf,
CC       cilia are completely absent from these ducts. On the other hand, cilia
CC       from the posterior pronephric ducts remain unaffected. Cilia biogenesis
CC       is also defective in the retina, with the outer segments of
CC       photoreceptor cells having modified cilia. No visible cilia in the
CC       lateral line organ. Pericardial edema is also observed at 5 dpf.
CC       {ECO:0000269|PubMed:22102889}.
CC   -!- SIMILARITY: Belongs to the CLUAP1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH97072.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR   EMBL; BC045921; AAH45921.1; -; mRNA.
DR   EMBL; BC067601; AAH67601.1; -; mRNA.
DR   EMBL; BC097072; AAH97072.1; ALT_SEQ; mRNA.
DR   RefSeq; NP_998173.1; NM_213008.1.
DR   AlphaFoldDB; Q7ZVC2; -.
DR   SMR; Q7ZVC2; -.
DR   STRING; 7955.ENSDARP00000101015; -.
DR   PaxDb; Q7ZVC2; -.
DR   GeneID; 406281; -.
DR   KEGG; dre:406281; -.
DR   CTD; 23059; -.
DR   ZFIN; ZDB-GENE-040426-1943; cluap1.
DR   eggNOG; KOG3647; Eukaryota.
DR   InParanoid; Q7ZVC2; -.
DR   OrthoDB; 874966at2759; -.
DR   PhylomeDB; Q7ZVC2; -.
DR   Reactome; R-DRE-5620924; Intraflagellar transport.
DR   PRO; PR:Q7ZVC2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR   GO; GO:0005929; C:cilium; IBA:GO_Central.
DR   GO; GO:0030992; C:intraciliary transport particle B; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR   GO; GO:0001822; P:kidney development; IMP:ZFIN.
DR   GO; GO:0045494; P:photoreceptor cell maintenance; IMP:ZFIN.
DR   InterPro; IPR019366; Clusterin-associated_protein-1.
DR   PANTHER; PTHR21547; PTHR21547; 1.
DR   Pfam; PF10234; Cluap1; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..427
FT                   /note="Clusterin-associated protein 1 homolog"
FT                   /id="PRO_0000239455"
FT   REGION          300..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          202..307
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        300..315
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        365..399
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        404..420
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        187
FT                   /note="A -> V (in Ref. 1; AAH45921)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   427 AA;  49127 MW;  590E280D300B976C CRC64;
     MSFRDLRNFT EMMRALGYPR LISMENFRSP NFPLVAEILI WLVKRYEPQM EIPSDVDTES
     DRVFFIKAVA QFMATKAHVK LNLKRLYQAD GYAVKEMLKI TSILYNAMKT KENAGGDQNN
     DENSKFKFDL GSKIADLKLA RQLGSEITAK GAALFDLLGQ EEDLRESRTA AIARPLEITE
     TERAMRAAVK DVTESIQMTK DLLNNVSSDE ASLEAKIEKK KQDLERNQKR LQTLQSVRPA
     FMDEYEKIEE DLEKQYQTYV EKYRNLSFLE QQLDDYHRVE QERFEEAEMA MKMRQNKLKE
     EEKRLMRSGV ARDEDSDVDI PEDEGSDSDI DDGQQARPHH PRHTQISGRG GARFIGSMRG
     GDSEETEDSE IDVDDDDEDD DEDGEEDEEE NEDLDEDNDS LEGSSGKPGR TNQSLHPQIL
     EESDNDF