CLU_ARATH
ID CLU_ARATH Reviewed; 1407 AA.
AC F4J5S1; F4J5S2; Q9SUZ1;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Clustered mitochondria protein {ECO:0000305|PubMed:14617080};
DE AltName: Full=Friendly mitochondria protein {ECO:0000303|PubMed:14617080};
GN Name=FMT {ECO:0000303|PubMed:14617080};
GN Synonyms=CLU {ECO:0000305|PubMed:14617080};
GN OrderedLocusNames=At3g52140 {ECO:0000312|Araport:AT3G52140};
GN ORFNames=F4F15.250 {ECO:0000312|EMBL:CAB41334.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 952-1407.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=14617080; DOI=10.1046/j.1365-313x.2003.01894.x;
RA Logan D.C., Scott I., Tobin A.K.;
RT "The genetic control of plant mitochondrial morphology and dynamics.";
RL Plant J. 36:500-509(2003).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26862170; DOI=10.1073/pnas.1515741113;
RA Larkin R.M., Stefano G., Ruckle M.E., Stavoe A.K., Sinkler C.A.,
RA Brandizzi F., Malmstrom C.M., Osteryoung K.W.;
RT "REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to
RT establish the size of the chloroplast compartment.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:E1116-E1125(2016).
CC -!- FUNCTION: mRNA-binding protein involved in proper cytoplasmic
CC distribution of mitochondria (By similarity) (PubMed:14617080).
CC Together with REC2, REC3 and FMT/CLU, contributes to the establishment
CC of the cellular volume devoted to the chloroplast compartment
CC (PubMed:26862170). {ECO:0000255|HAMAP-Rule:MF_03013,
CC ECO:0000269|PubMed:14617080, ECO:0000269|PubMed:26862170}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03013}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4J5S1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4J5S1-2; Sequence=VSP_044168;
CC -!- DISRUPTION PHENOTYPE: Causes mitochondria to cluster within cells
CC (PubMed:14617080). Reduced proportion of the cellular volume devoted to
CC chloroplasts leading to an abnormal chloroplasts distributions
CC (PubMed:26862170). Lower levels of chlorophyll, especially in plants
CC lacking REC1, REC2, REC3 and FMT/CLU (PubMed:26862170).
CC {ECO:0000269|PubMed:14617080, ECO:0000269|PubMed:26862170}.
CC -!- SIMILARITY: Belongs to the CLU family. {ECO:0000255|HAMAP-
CC Rule:MF_03013}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB41334.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL049711; CAB41334.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78899.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78900.1; -; Genomic_DNA.
DR EMBL; BX824152; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T49093; T49093.
DR RefSeq; NP_001190066.1; NM_001203137.1. [F4J5S1-1]
DR RefSeq; NP_190782.3; NM_115073.5. [F4J5S1-2]
DR AlphaFoldDB; F4J5S1; -.
DR SMR; F4J5S1; -.
DR BioGRID; 9696; 6.
DR STRING; 3702.AT3G52140.4; -.
DR iPTMnet; F4J5S1; -.
DR PaxDb; F4J5S1; -.
DR PRIDE; F4J5S1; -.
DR EnsemblPlants; AT3G52140.1; AT3G52140.1; AT3G52140. [F4J5S1-2]
DR EnsemblPlants; AT3G52140.2; AT3G52140.2; AT3G52140. [F4J5S1-1]
DR GeneID; 824378; -.
DR Gramene; AT3G52140.1; AT3G52140.1; AT3G52140. [F4J5S1-2]
DR Gramene; AT3G52140.2; AT3G52140.2; AT3G52140. [F4J5S1-1]
DR KEGG; ath:AT3G52140; -.
DR Araport; AT3G52140; -.
DR eggNOG; KOG1839; Eukaryota.
DR InParanoid; F4J5S1; -.
DR OMA; VSGFYVN; -.
DR PRO; PR:F4J5S1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J5S1; baseline and differential.
DR Genevisible; F4J5S1; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0019750; P:chloroplast localization; IMP:UniProtKB.
DR GO; GO:0007005; P:mitochondrion organization; IEA:UniProtKB-UniRule.
DR CDD; cd15466; CLU-central; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 3.30.2280.10; -; 1.
DR HAMAP; MF_03013; CLU; 1.
DR InterPro; IPR033646; CLU-central.
DR InterPro; IPR025697; CLU_dom.
DR InterPro; IPR028275; CLU_N.
DR InterPro; IPR027523; CLU_prot.
DR InterPro; IPR007967; GSKIP_dom.
DR InterPro; IPR023231; GSKIP_dom_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR12601; PTHR12601; 1.
DR Pfam; PF13236; CLU; 1.
DR Pfam; PF15044; CLU_N; 1.
DR Pfam; PF05303; DUF727; 1.
DR Pfam; PF12807; eIF3_p135; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF103107; SSF103107; 1.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS51823; CLU; 1.
DR PROSITE; PS50005; TPR; 3.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..1407
FT /note="Clustered mitochondria protein"
FT /id="PRO_0000419436"
FT DOMAIN 384..670
FT /note="Clu"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01167"
FT REPEAT 1025..1058
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 1067..1100
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 1109..1142
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REPEAT 1151..1184
FT /note="TPR 4"
FT /evidence="ECO:0000255"
FT REPEAT 1193..1226
FT /note="TPR 5"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 83..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 724..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1358..1407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 729..760
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1366..1381
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 913..916
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044168"
FT CONFLICT 1183
FT /note="R -> K (in Ref. 3; BX824152)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1407 AA; 153689 MW; D3A3FEF898F4FDE1 CRC64;
MAGKSNKSKA KRAAQSTTTN STTDVKSDAP APPVAATVPA TAPVTAAAAP VATAAAPVTA
PDNGTLTAVD SAVPEANEVA PTIPKADESE SQVENNDAQP KQGELRLYPV SVKTQSGGKM
ELQLNPGDSV MDIRQFLLDA PETCYFTCYE LLLRNKDGET HHLEDYNEIS EVADITIGGC
SLEMVAALYD DRSIRAHVHR ARDLLSLSTL HSSLSTTLAL QYDAALNKVQ NPGDKPKSDV
PELECLGFME DVPGSLKKLI NSTSEEIRSV ENIVFSSFNP PPSHRRLVGD LIYLDVVTLE
GNKYCITGTT KTFYVNSSSG NILDPRPSKS GFEAATLIGL LQKLSSKFKK AFREVMEKKA
SAHPFENVQS LLPPHSWLRT YPVPDHKRDA ARAEEALTIS YGSELIGMQR DWNEELQSCR
EFPHTSPQER ILRDRALYKV SSDFVDAALN GAIGVISRCI PPINPTDPEC LHMYVHNNIF
FSFAVDADIE QLSKKRPSNQ MTEKVSSSEK VSCTEGTCDN EEHNNCNEAP LVENEQATYA
SANNDLKGTK LYQEADVPGL YNLAMAIIDY RGHRVVAQSV LPGILQGDKS DALLYGSVDN
GKKICWNEDF HAKVLEAAKL LHIKEHSVID ASETVFKLAA PVECKGIVGS DNRHYLLDLM
RVTPRDANYT GPESRFCVLR PELITSFCQA ESLEKSKFKT KADEGGDDSS NVSADTSKVG
DALIDGEANG ASNSDQKSIS DKQNTTAEDY AAGSSESSKS CDQIAFNPNV FTDFTLGGNQ
EEIAADEENV KKVSSYLVDV VLPKFIEDLC TLEVSPMDGQ TLTEALHAHG VNVRYIGRVA
NGVKHLPHLW DLCLNEITVR SAKHILKDIL RDIEDHDIGS AVSHFLNCFF GNYQTAGGKA
SANSSTAKNQ KKFFGADQPI TKKGQGRGKG KASSKKSFSS YMMVDSNILW SDIQEFAKAK
YEFELPELSR TTAKKVSVLR NLCQKVGVSI AARKYDFSAN TPFETSDILD LRPVIKHSVP
VCSEAKDLVE MGKVQLAEGM LSESYTFFSE AFSILQQVTG PMHREVANCC RYLAMVLYHA
GDMAGAIMQQ HKELIINERC LGLDHPDTAH SYGNMALFYH GLNQTELALQ NMGRALLLLG
LSSGPDHPDV AATFINVAMM YQDMGKMDTA LRYLQEALKK NERLLGPEHI QTAVCYHALA
IAFNCMGAFK LSHQHEKKTY DILVKQLGDD DSRTRDSLNW MKTFKMRELQ MTAQKQKGQA
ANAANTQKAI DLLKAHPDLI HAFQNAAATG RTNALNSAVL GETQPRGRGF DERAARAAAE
VRKKAAAKGL LVRPQGGVPV QAMPPLSQLQ NMINTATVSS EKGGENGEAK VQEKKESSEN
GKTENLAPAG LGAGLTSLDK KKQKAKK
