CLU_DROME
ID CLU_DROME Reviewed; 1448 AA.
AC A1ZAB5; Q6NNE5;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein clueless {ECO:0000255|HAMAP-Rule:MF_03013};
DE AltName: Full=Clustered mitochondria protein homolog {ECO:0000255|HAMAP-Rule:MF_03013};
GN Name=clu {ECO:0000255|HAMAP-Rule:MF_03013}; ORFNames=CG8443;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=19638420; DOI=10.1242/dmm.002378;
RA Cox R.T., Spradling A.C.;
RT "Clueless, a conserved Drosophila gene required for mitochondrial
RT subcellular localization, interacts genetically with parkin.";
RL Dis. Model. Mech. 2:490-499(2009).
CC -!- FUNCTION: mRNA-binding protein involved in proper cytoplasmic
CC distribution of mitochondria. {ECO:0000255|HAMAP-Rule:MF_03013,
CC ECO:0000269|PubMed:19638420}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03013,
CC ECO:0000269|PubMed:19638420}.
CC -!- DISRUPTION PHENOTYPE: Causes mitochondria to cluster within cells.
CC {ECO:0000269|PubMed:19638420}.
CC -!- SIMILARITY: Belongs to the CLU family. {ECO:0000255|HAMAP-
CC Rule:MF_03013}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE013599; AAF58047.1; -; Genomic_DNA.
DR EMBL; BT011344; AAR96136.1; -; mRNA.
DR RefSeq; NP_611095.1; NM_137251.3.
DR AlphaFoldDB; A1ZAB5; -.
DR SMR; A1ZAB5; -.
DR BioGRID; 62513; 27.
DR IntAct; A1ZAB5; 6.
DR MINT; A1ZAB5; -.
DR STRING; 7227.FBpp0086380; -.
DR iPTMnet; A1ZAB5; -.
DR PaxDb; A1ZAB5; -.
DR PRIDE; A1ZAB5; -.
DR EnsemblMetazoa; FBtr0087242; FBpp0086380; FBgn0034087.
DR GeneID; 36793; -.
DR KEGG; dme:Dmel_CG8443; -.
DR UCSC; CG8443-RA; d. melanogaster.
DR CTD; 1191; -.
DR FlyBase; FBgn0034087; clu.
DR VEuPathDB; VectorBase:FBgn0034087; -.
DR eggNOG; KOG1839; Eukaryota.
DR InParanoid; A1ZAB5; -.
DR OMA; VSGFYVN; -.
DR OrthoDB; 60958at2759; -.
DR PhylomeDB; A1ZAB5; -.
DR SignaLink; A1ZAB5; -.
DR BioGRID-ORCS; 36793; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36793; -.
DR PRO; PR:A1ZAB5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034087; Expressed in egg chamber and 54 other tissues.
DR ExpressionAtlas; A1ZAB5; baseline and differential.
DR Genevisible; A1ZAB5; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0031315; C:extrinsic component of mitochondrial outer membrane; IPI:FlyBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
DR GO; GO:0072686; C:mitotic spindle; IDA:FlyBase.
DR GO; GO:0098799; C:outer mitochondrial membrane protein complex; IDA:FlyBase.
DR GO; GO:0003729; F:mRNA binding; IDA:FlyBase.
DR GO; GO:0043022; F:ribosome binding; IDA:FlyBase.
DR GO; GO:0055059; P:asymmetric neuroblast division; IMP:FlyBase.
DR GO; GO:0048312; P:intracellular distribution of mitochondria; IBA:GO_Central.
DR GO; GO:0051646; P:mitochondrion localization; IMP:FlyBase.
DR CDD; cd15466; CLU-central; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 3.30.2280.10; -; 1.
DR HAMAP; MF_03013; CLU; 1.
DR InterPro; IPR033646; CLU-central.
DR InterPro; IPR025697; CLU_dom.
DR InterPro; IPR028275; CLU_N.
DR InterPro; IPR027523; CLU_prot.
DR InterPro; IPR007967; GSKIP_dom.
DR InterPro; IPR023231; GSKIP_dom_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12601; PTHR12601; 1.
DR Pfam; PF13236; CLU; 1.
DR Pfam; PF15044; CLU_N; 1.
DR Pfam; PF05303; DUF727; 1.
DR Pfam; PF12807; eIF3_p135; 1.
DR SUPFAM; SSF103107; SSF103107; 1.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS51823; CLU; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..1448
FT /note="Protein clueless"
FT /id="PRO_0000366382"
FT DOMAIN 424..666
FT /note="Clu"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01167"
FT REGION 1..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 726..773
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 958..1010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1414..1448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 747..771
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 982..1010
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 270
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT CONFLICT 495
FT /note="Missing (in Ref. 3; AAR96136)"
FT /evidence="ECO:0000305"
FT CONFLICT 959
FT /note="V -> L (in Ref. 3; AAR96136)"
FT /evidence="ECO:0000305"
FT CONFLICT 1061
FT /note="K -> R (in Ref. 3; AAR96136)"
FT /evidence="ECO:0000305"
FT CONFLICT 1323
FT /note="T -> A (in Ref. 3; AAR96136)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1448 AA; 160852 MW; A9D1A150F6B8CBBB CRC64;
MALETEAKNS NATATGDATA TATKASGKAK ENNNTAGGKK NLNPNSNQQN SNQNLVNGNG
TAADGPAAKK KGKKNRNKSP TEPTTEAVLS NGHAEKPTVV DAVEDNADTN ANVEKPQEGG
APDAEADGDD IDLDALQDIG ITVNISSPGA DLLCVQLSSM ELVQEIHQLL MDREETCHRT
CFSLQLDNVT LDNFAELKSI NNLEQGSTIK VVEEPYTMRE ARIHVRHVRD LLKNLDPADA
YNGIDCTSLT YLNTITQGDL LDKKRTRPDS VDCTPPEYVT PGVSDPPILP LHPNVKNAKG
PQALKVLTTS AWNPPPGPRK LHGDLMYLYV VTMEDKRFHI SACSKGFFIN QSTDDTFNPK
PDNPSHLSHS LIDLLSHISP SFRRAFQTIQ KRRTMRHAFE RVATPYQVYQ WAAPILEHTV
DAIRAEDAFS SKLGYEEHIP GQTRDWNEEL QTTRELPRKT LPERLLRERA IFKVHGDFVT
AATRGAMAVI DGNVLAINPG EDTKMQMFIW NNIFFSMGFD VRDHYKELGG DAAAFVAPRY
DLHGVRVYNA VDIEGLYTLG TVVVDYRGYR VTAQSIIPGI LEREQEQSVV YGSIDFGKTV
LSHPKYLELL RQAGKHLKIL PHVVLNERDE PVELCSSVEC KGIIGNDGRH YILDLLRTFP
PDVNFLKLQD VQLSKELVDM GFPIEHRHKL CCLRQELLEA FIEDRHVNFI RIAAARLQQL
TTIKQSEKSE ANPVPALEGA EAASKVNGAE KPDDKEKKNE EEEKKERSTS GEARAAAIVN
AIREAQSNVA TSNEVQAAEV VKRACAAVGS LKEKEFDFRF NPDVFSPGIR HADGEEGTSL
AKQKVLVQEA AEFLVLKQIP AFIKEHMSHS SSPIDGQSLT ESLHSHGINV RYLGKVIKIL
SQMPRMDYLH RIAVLELIVR ATKHIYYTYM QNTEPLHLSA AISHFLNCLL TNGPVNPAVS
SEEAHKKRGN GGKHNKHKSS KGGKGQQQQQ TTGNQNGSSS GSSNSSSASD WTLMTPRSLW
QQIRKEAKVY WDWELDCDSI ETAVSKYGIL RISLMRAFCL KVGIQVLLRE YNFESKHKPT
FGDDDIVNVF PIVKHISPRA TDAYNFYTTG QAKIQQGLFK EGYELISGAL NLLNNVFGAL
HQENGSCLRM LARLSYLLGD AQDALAIQQR AVIMSERVNG MDHPSTILEY THLSLYSFAN
GHVGMSLKLL YRARYLMVLI CGEDHPEVAL IDSNISLILH ALGEYELSLR FIEHALKLNL
KYFGDKDMHV ALSYHLMART QSCMGDFRSA LNNEKETYSF YKSQLGENHE KTRDSAECLR
LLTQQAVLLQ RKMNDIYSSG KLTSDLPPIH ITPPSMGSVL DMLNTINGIL FVKISRKDIV
KVRSEIEKHF KTDSTENEVN DAINSIVAAA NNNGEAEDAV SKDIKEQPEA GKQLTNGDKA
AATEATSS
