CLU_MOUSE
ID CLU_MOUSE Reviewed; 1315 AA.
AC Q5SW19; A8Y5E2; Q3U014; Q3U4M5; Q3V3Q8; Q505Q6; Q6GQX1; Q6PAT1; Q8CHD9;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Clustered mitochondria protein homolog {ECO:0000255|HAMAP-Rule:MF_03013};
GN Name=Cluh; Synonyms=Kiaa0664;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone, Dendritic cell, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 226-1315 (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-1315 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12465718; DOI=10.1093/dnares/9.5.179;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 9:179-188(2002).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: mRNA-binding protein involved in proper cytoplasmic
CC distribution of mitochondria. Specifically binds mRNAs of nuclear-
CC encoded mitochondrial proteins in the cytoplasm and regulates transport
CC or translation of these transcripts close to mitochondria, playing a
CC role in mitochondrial biogenesis. {ECO:0000255|HAMAP-Rule:MF_03013}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03013}.
CC Cytoplasmic granule {ECO:0000255|HAMAP-Rule:MF_03013}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5SW19-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5SW19-2; Sequence=VSP_036579, VSP_036580;
CC Name=3;
CC IsoId=Q5SW19-3; Sequence=VSP_036578;
CC -!- SIMILARITY: Belongs to the CLU family. {ECO:0000255|HAMAP-
CC Rule:MF_03013}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH72573.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE20503.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE32406.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE32929.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE34042.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAI24374.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAP19360.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AK036540; BAE20503.1; ALT_INIT; mRNA.
DR EMBL; AK154146; BAE32406.1; ALT_INIT; mRNA.
DR EMBL; AK154925; BAE32929.1; ALT_INIT; mRNA.
DR EMBL; AK157310; BAE34042.1; ALT_INIT; mRNA.
DR EMBL; AL607024; CAI24374.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL607024; CAP19360.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC060069; AAH60069.1; -; mRNA.
DR EMBL; BC072573; AAH72573.1; ALT_INIT; mRNA.
DR EMBL; BC094446; AAH94446.1; -; mRNA.
DR EMBL; AB093256; BAC41440.3; -; Transcribed_RNA.
DR RefSeq; NP_001074627.1; NM_001081158.2.
DR RefSeq; XP_006534416.1; XM_006534353.3. [Q5SW19-1]
DR RefSeq; XP_006534417.1; XM_006534354.3.
DR AlphaFoldDB; Q5SW19; -.
DR SMR; Q5SW19; -.
DR BioGRID; 216526; 223.
DR IntAct; Q5SW19; 3.
DR STRING; 10090.ENSMUSP00000090593; -.
DR iPTMnet; Q5SW19; -.
DR PhosphoSitePlus; Q5SW19; -.
DR SwissPalm; Q5SW19; -.
DR EPD; Q5SW19; -.
DR jPOST; Q5SW19; -.
DR MaxQB; Q5SW19; -.
DR PaxDb; Q5SW19; -.
DR PeptideAtlas; Q5SW19; -.
DR PRIDE; Q5SW19; -.
DR ProteomicsDB; 283530; -. [Q5SW19-1]
DR ProteomicsDB; 283531; -. [Q5SW19-2]
DR ProteomicsDB; 283532; -. [Q5SW19-3]
DR GeneID; 74148; -.
DR KEGG; mmu:74148; -.
DR UCSC; uc007kca.1; mouse. [Q5SW19-1]
DR CTD; 23277; -.
DR MGI; MGI:1921398; Cluh.
DR eggNOG; KOG1839; Eukaryota.
DR InParanoid; Q5SW19; -.
DR OrthoDB; 60958at2759; -.
DR PhylomeDB; Q5SW19; -.
DR TreeFam; TF300565; -.
DR BioGRID-ORCS; 74148; 10 hits in 74 CRISPR screens.
DR ChiTaRS; Cluh; mouse.
DR PRO; PR:Q5SW19; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q5SW19; protein.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR GO; GO:0048312; P:intracellular distribution of mitochondria; ISS:UniProtKB.
DR GO; GO:0007005; P:mitochondrion organization; ISS:UniProtKB.
DR CDD; cd15466; CLU-central; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR Gene3D; 3.30.2280.10; -; 1.
DR HAMAP; MF_03013; CLU; 1.
DR InterPro; IPR033646; CLU-central.
DR InterPro; IPR025697; CLU_dom.
DR InterPro; IPR028275; CLU_N.
DR InterPro; IPR027523; CLU_prot.
DR InterPro; IPR023231; GSKIP_dom_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR12601; PTHR12601; 1.
DR Pfam; PF13236; CLU; 1.
DR Pfam; PF15044; CLU_N; 1.
DR Pfam; PF12807; eIF3_p135; 1.
DR SUPFAM; SSF103107; SSF103107; 1.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS51823; CLU; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome;
KW Repeat; TPR repeat.
FT CHAIN 1..1315
FT /note="Clustered mitochondria protein homolog"
FT /id="PRO_0000366373"
FT DOMAIN 335..577
FT /note="Clu"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01167"
FT REPEAT 980..1013
FT /note="TPR 1"
FT REPEAT 1022..1055
FT /note="TPR 2"
FT REPEAT 1106..1139
FT /note="TPR 3"
FT REPEAT 1148..1181
FT /note="TPR 4"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 638..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1268..1315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 638..656
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1268..1295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75153"
FT MOD_RES 281
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75153"
FT MOD_RES 655
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75153"
FT MOD_RES 725
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75153"
FT VAR_SEQ 761..811
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_036578"
FT VAR_SEQ 762..856
FT /note="KDCTEYAVLPMDGATLAEVMRQRGINMRYLGKVLDLVLRSPARDQLDHIYKI
FT GIGELITRSAKHIFKTYLQGVELSGLSAAISHFLNCFLSSYPN -> REGLKGWVGAAE
FT RGCCPLVPPVPWFLDHIENLLVTSALASPSLAHWQCPISQRRRQI (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036579"
FT VAR_SEQ 857..1315
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036580"
FT CONFLICT 412
FT /note="E -> D (in Ref. 3; AAH72573)"
FT /evidence="ECO:0000305"
FT CONFLICT 817
FT /note="E -> D (in Ref. 1; BAE32406)"
FT /evidence="ECO:0000305"
FT CONFLICT 1284
FT /note="A -> APE (in Ref. 1; BAE34042 and 4; BAC41440)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1315 AA; 148068 MW; 868CC53BDA8E3A65 CRC64;
MLLNGDCSEN LKKEEGTSEP PRENGLDEGE PGDETTGQEV IVIQDTGFSV KILAPGIEPF
SLQVSPQEMV QEIHQVLMDR EDTCHRTCFS LHLDGNMLDH FSELRSVEGL QEGSVLRVVE
EPYTVREARI HVRHVRDLLK SLDPSDAFNG VDCNSLSFLS VFTDGDLGDS GKRKKGLEMD
PIDCTPPEYI LPGSRERPLC PLQPQNRDWK PLQCLKVLTM SGWNPPPGNR KMHGDLMYLF
VITAEDRQVS ITASTRGFYL NQSTAYHFNP KPASPRFLSH SLVELLNQIS PTFKKNFAVL
QKKRVQRHPF ERIATPFQVY SWTAPQAEHA MDCVRAEDAY TSRLGYEEHI PGQTRDWNEE
LQTTRELPRK NLPERLLRER AIFKVHSDFT AAATRGAMAV IDGNVMAINP SEETKMQMFI
WNNIFFSLGF DVRDHYKDFG GDVAAYVAPT NDLNGVRTYN AVDVEGLYTL GTVVVDYRGY
RVTAQSIIPG ILERDQEQSV IYGSIDFGKT VVSHPRYLEL LERTSRPLKI LRHRVLNDRD
EEVELCSSVE CKGIIGNDGR HYILDLLRTF PPDLNFLPVP GEELPEECSR AGFPRTHRHK
LCCLRQELVD AFVEHRYLLF MKLAALQLMQ QKASKVETTT SLENGGLPSS AETKSEDSIG
PEAGCEEEGS SVSGLAKVKE LAETIASDDG TVDPRSREVI RNACKAVGSI SSTAFDIRFN
PDIFSPGVRF PESCQDEVRD QKQLLKDAAA FLLSCQIPGL VKDCTEYAVL PMDGATLAEV
MRQRGINMRY LGKVLDLVLR SPARDQLDHI YKIGIGELIT RSAKHIFKTY LQGVELSGLS
AAISHFLNCF LSSYPNPVAH LPADELLSKK RNKRRKNRPP GAADNTAWAV MTPQELWKNI
CHEAKNYFDF TLECDSVDQA VETYGLQKIT LLREISLKTG IQILLKEYSF DSRHKPAFTE
EDVLNIFPVV KHVNPKASDA FHFFQSGQAK VQQGFLKEGC ELINEALNLF NNVYGAMHVE
ICACLRLLAR LHYIMGDYAE ALSNQQKAVL MSERVMGIEH PNTIQEYMHL ALYCFASSQL
STALSLLYRA RYLMLLVFGE DHPEMALLDN NIGLVLHGVM EYDLSLRFLE NALAVTTKYH
GPKALKVALS HHLVARVYES KAEFRSALQH EKEGYTIYKT QLGEDHEKTK ESSEYLKCLT
QQAVALQRTM NEIYRNGSSA NIPPLKFTAP SMTSVLEQLN VINGILFIPL SQKDLENLKA
EVARRHQLQE ANRNRDKAEE QPMAPEPEPE PERAVEDMGS PQTAKEGPSS LNLQG
