CLXN_BOVIN
ID CLXN_BOVIN Reviewed; 212 AA.
AC Q32L26;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Calaxin {ECO:0000250|UniProtKB:Q9D3N2};
DE AltName: Full=EF-hand calcium-binding domain-containing protein 1;
GN Name=CLXN; Synonyms=EFCAB1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0007744|PDB:7RRO}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), SUBUNIT, FUNCTION,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT "De novo identification of mammalian ciliary motility proteins using cryo-
RT EM.";
RL Cell 184:5791-5806.e19(2021).
CC -!- FUNCTION: Component of the outer dynein arm-docking complex (ODA-DC)
CC that mediates outer dynein arms (ODA) binding onto the doublet
CC microtubule. Seems to regulate the assembly of both ODAs and their
CC axonemal docking complex onto ciliary microtubules (PubMed:34715025).
CC Regulates ciliary and flagellar motility and is required for cilia-
CC driven determination of body laterality (By similarity).
CC {ECO:0000250|UniProtKB:Q9D3N2, ECO:0000269|PubMed:34715025}.
CC -!- SUBUNIT: Component of the outer dynein arm-docking complex along with
CC ODAD1, ODAD2, ODAD3 and ODAD4. {ECO:0000269|PubMed:34715025}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:Q96M63}.
CC -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC {ECO:0000269|PubMed:34715025}.
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DR EMBL; BC109799; AAI09800.1; -; mRNA.
DR RefSeq; NP_001032674.1; NM_001037585.2.
DR PDB; 7RRO; EM; 3.40 A; I3/I4=1-212.
DR PDBsum; 7RRO; -.
DR AlphaFoldDB; Q32L26; -.
DR SMR; Q32L26; -.
DR STRING; 9913.ENSBTAP00000011457; -.
DR PaxDb; Q32L26; -.
DR Ensembl; ENSBTAT00000011457; ENSBTAP00000011457; ENSBTAG00000008693.
DR Ensembl; ENSBTAT00000066916; ENSBTAP00000073883; ENSBTAG00000008693.
DR GeneID; 505272; -.
DR KEGG; bta:505272; -.
DR CTD; 79645; -.
DR VEuPathDB; HostDB:ENSBTAG00000008693; -.
DR VGNC; VGNC:28339; CLXN.
DR eggNOG; KOG0044; Eukaryota.
DR GeneTree; ENSGT00940000159968; -.
DR HOGENOM; CLU_061288_3_2_1; -.
DR InParanoid; Q32L26; -.
DR OMA; FTENIRH; -.
DR OrthoDB; 1331864at2759; -.
DR TreeFam; TF323358; -.
DR Proteomes; UP000009136; Chromosome 14.
DR Bgee; ENSBTAG00000008693; Expressed in olfactory segment of nasal mucosa and 67 other tissues.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0120228; C:outer dynein arm docking complex; ISS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003341; P:cilium movement; IDA:UniProtKB.
DR GO; GO:0036158; P:outer dynein arm assembly; IDA:UniProtKB.
DR GO; GO:0003352; P:regulation of cilium movement; ISS:UniProtKB.
DR GO; GO:1901317; P:regulation of flagellated sperm motility; ISS:UniProtKB.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR028846; Recoverin.
DR PANTHER; PTHR23055; PTHR23055; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Cell projection; Cytoplasm; Cytoskeleton;
KW Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..212
FT /note="Calaxin"
FT /id="PRO_0000251968"
FT DOMAIN 65..100
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 101..136
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 146..181
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 78
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 80
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 82
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 84
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 89
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 114
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 116
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 118
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 125
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 165
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
SQ SEQUENCE 212 AA; 24635 MW; BC72F75EEBC664A4 CRC64;
MNRKKLQKLT DSLTKNCKHF SKFEVKCLIN LFYNLVGEVT ERQGVIIGLD RNAFRNILHM
TFGMTDDMIM DRVFRGFDKD NDGCISVTEW VYGLSVFLRG TLEEKMKYCF EVFDLNGDSF
ISKEEMFHML KNSLLKQPSE EDPDEGIKDL VEITLKKMDH DHDGKLSFAD YEQAVREETL
LLEAFGPCLP DPKSQSEFEA QVFKDPNEFN EV
