ID   CLY_CONMS               Reviewed;          23 AA.
AC   P0C8S6;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Conolysin-Mt1;
DE   AltName: Full=Moonwalker peptide;
DE   Contains:
DE     RecName: Full=Conolysin-Mt2;
OS   Conus mustelinus (Weasel cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Rhizoconus.
OX   NCBI_TaxID=101309;
RN   [1]
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AMIDATION AT SER-22, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=17927208; DOI=10.1021/bi700775p;
RA   Biggs J.S., Rosenfeld Y., Shai Y., Olivera B.M.;
RT   "Conolysin-Mt: a conus peptide that disrupts cellular membranes.";
RL   Biochemistry 46:12586-12593(2007).
CC   -!- FUNCTION: This cytolytic peptide has ability to disrupt the integrity
CC       of cell membranes from both prokaryotes and eukaryotes. It
CC       permeabilizes both negatively charged prokaryotic (PE:PG) and
CC       zwitterionic eukaryotic (PC:cholesterol) model membranes. It has potent
CC       hemolytic activity on human erythrocytes and exhibits low antimicrobial
CC       activity against the Gram-negative bacterium E.coli (MIC>50 uM) and the
CC       Gram-positive bacterium S.aureus (MIC=25-50 uM). Intracranial injection
CC       causes mice to shuffle backward until the encounter an obstacle, at
CC       which time the mouse jump into the air. The backward shuffle is
CC       reminiscent to the signature dance 'moonwalk' that gained widespread
CC       popularity after being performed by Michael Jackson.
CC       {ECO:0000269|PubMed:17927208}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17927208}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000269|PubMed:17927208}.
CC   -!- MASS SPECTROMETRY: [Conolysin-Mt1]: Mass=2750.40; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17927208};
CC   -!- MASS SPECTROMETRY: [Conolysin-Mt2]: Mass=2692.45; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17927208};
CC   -!- MISCELLANEOUS: The mature peptide does not contain cysteine residue.
CC   -!- MISCELLANEOUS: Mt1 and Mt2 peptides are found in a ratio of 1:4. Mt1 is
CC       more active when intracranially injected.
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DR   AlphaFoldDB; P0C8S6; -.
DR   ConoServer; 2746; conolysin-Mt1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW   Hemolysis; Secreted; Toxin.
FT   PEPTIDE         1..23
FT                   /note="Conolysin-Mt1"
FT                   /id="PRO_0000366033"
FT   PEPTIDE         1..22
FT                   /note="Conolysin-Mt2"
FT                   /id="PRO_0000366034"
FT   MOD_RES         22
FT                   /note="Serine amide"
FT                   /evidence="ECO:0000269|PubMed:17927208"
SQ   SEQUENCE   23 AA;  2750 MW;  08C3880734C01BF4 CRC64;
     FHPSLWVLIP QYIQLIRKIL KSG