ID   CM2A1_DICDI             Reviewed;         206 AA.
AC   Q54GK9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Charged multivesicular body protein 2a homolog 1;
DE   AltName: Full=Vacuolar protein-sorting-associated protein 2A;
GN   Name=chmp2a1; Synonyms=vps2A; ORFNames=DDB_G0290087;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Probable core component of the endosomal sorting required for
CC       transport complex III (ESCRT-III) which is involved in multivesicular
CC       bodies (MVBs) formation and sorting of endosomal cargo proteins into
CC       MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by
CC       invagination and scission from the limiting membrane of the endosome
CC       and are delivered to lysosomes enabling degradation of membrane
CC       proteins (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Probable core component of the endosomal sorting required for
CC       transport complex III (ESCRT-III). ESCRT-III components are thought to
CC       multimerize to form a flat lattice on the perimeter membrane of the
CC       endosome (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
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DR   EMBL; AAFI02000152; EAL62404.2; -; Genomic_DNA.
DR   RefSeq; XP_635908.2; XM_630816.2.
DR   AlphaFoldDB; Q54GK9; -.
DR   SMR; Q54GK9; -.
DR   STRING; 44689.DDB0234032; -.
DR   PaxDb; Q54GK9; -.
DR   PRIDE; Q54GK9; -.
DR   EnsemblProtists; EAL62404; EAL62404; DDB_G0290087.
DR   GeneID; 8627474; -.
DR   KEGG; ddi:DDB_G0290087; -.
DR   dictyBase; DDB_G0290087; vps2A.
DR   eggNOG; KOG3230; Eukaryota.
DR   HOGENOM; CLU_069208_1_2_1; -.
DR   InParanoid; Q54GK9; -.
DR   OMA; NMREFQM; -.
DR   PhylomeDB; Q54GK9; -.
DR   Reactome; R-DDI-1632852; Macroautophagy.
DR   Reactome; R-DDI-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR   Reactome; R-DDI-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR   PRO; PR:Q54GK9; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0000815; C:ESCRT III complex; ISS:dictyBase.
DR   GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR   GO; GO:0032509; P:endosome transport via multivesicular body sorting pathway; IBA:GO_Central.
DR   GO; GO:0045324; P:late endosome to vacuole transport; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IBA:GO_Central.
DR   InterPro; IPR005024; Snf7_fam.
DR   PANTHER; PTHR10476; PTHR10476; 1.
DR   Pfam; PF03357; Snf7; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Endosome; Membrane; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..206
FT                   /note="Charged multivesicular body protein 2a homolog 1"
FT                   /id="PRO_0000328591"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          12..80
FT                   /evidence="ECO:0000255"
FT   COILED          114..148
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        10..32
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   206 AA;  23677 MW;  7A1AC9FC69DF7C5F CRC64;
     MSFFGGNKKT PEQELKDSKR ELSKGQREMD RELNRLKIVE QEYIGKIKQL AKAGRNDEAK
     RMANDLVKLR GQMERMRATK TTLSAVSTKT TTIKANQTMA NAMASATKAM STMNKQTDLV
     QLQKTMMEYE KQTQRVEMTE EMMQDMFEDD EVDEEADDIL SKVVDEVCLD NYQKMPSVAQ
     KDLPYSSKTS TFKTEDEELN KLLQSL