CM2_MAIZE
ID CM2_MAIZE Reviewed; 253 AA.
AC B4FUP5; B4FAF1;
DT 10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Chorismate mutase 2, cytosolic {ECO:0000305};
DE Short=ZmCM2 {ECO:0000303|PubMed:21976020};
DE EC=5.4.99.5 {ECO:0000255|PROSITE-ProRule:PRU00516, ECO:0000269|PubMed:21976020};
GN Name=CM2 {ECO:0000303|PubMed:21976020};
GN ORFNames=ZEAMMB73_Zm00001d015509 {ECO:0000312|EMBL:AQK69073.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [4]
RP CATALYTIC ACTIVITY, ACTIVITY REGULATION, HOMODIMERIZATION, INTERACTION WITH
RP USTILAGO MAYDIS CMU1, AND SUBCELLULAR LOCATION.
RX PubMed=21976020; DOI=10.1038/nature10454;
RA Djamei A., Schipper K., Rabe F., Ghosh A., Vincon V., Kahnt J., Osorio S.,
RA Tohge T., Fernie A.R., Feussner I., Feussner K., Meinicke P.,
RA Stierhof Y.D., Schwarz H., Macek B., Mann M., Kahmann R.;
RT "Metabolic priming by a secreted fungal effector.";
RL Nature 478:395-398(2011).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS).
RX PubMed=30651637; DOI=10.1038/s41586-018-0857-9;
RA Han X., Altegoer F., Steinchen W., Binnebesel L., Schuhmacher J.,
RA Glatter T., Giammarinaro P.I., Djamei A., Rensing S.A., Reissmann S.,
RA Kahmann R., Bange G.;
RT "A kiwellin disarms the metabolic activity of a secreted fungal virulence
RT factor.";
RL Nature 565:650-653(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chorismate = prephenate; Xref=Rhea:RHEA:13897,
CC ChEBI:CHEBI:29748, ChEBI:CHEBI:29934; EC=5.4.99.5;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00516,
CC ECO:0000269|PubMed:21976020};
CC -!- ACTIVITY REGULATION: No allosteric regulation.
CC {ECO:0000269|PubMed:21976020}.
CC -!- PATHWAY: Metabolic intermediate biosynthesis; prephenate biosynthesis;
CC prephenate from chorismate: step 1/1. {ECO:0000250|UniProtKB:P42738}.
CC -!- SUBUNIT: Homodimer (PubMed:21976020). Interacts with Cmu1 of the fungal
CC pathogen Ustilago maydis (PubMed:21976020).
CC {ECO:0000269|PubMed:21976020}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:21976020}.
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DR EMBL; CM000781; AQK69073.1; -; Genomic_DNA.
DR EMBL; EU951879; ACG23997.1; -; mRNA.
DR EMBL; BT034089; ACF79094.1; -; mRNA.
DR EMBL; BT040833; ACF85838.1; -; mRNA.
DR RefSeq; NP_001130752.1; NM_001137280.1.
DR PDB; 6H3P; X-ray; 2.70 A; A/B=1-253.
DR PDBsum; 6H3P; -.
DR AlphaFoldDB; B4FUP5; -.
DR SMR; B4FUP5; -.
DR STRING; 4577.GRMZM2G179454_P01; -.
DR PaxDb; B4FUP5; -.
DR PRIDE; B4FUP5; -.
DR EnsemblPlants; Zm00001eb234080_T001; Zm00001eb234080_P001; Zm00001eb234080.
DR GeneID; 100191856; -.
DR Gramene; Zm00001eb234080_T001; Zm00001eb234080_P001; Zm00001eb234080.
DR KEGG; zma:100191856; -.
DR eggNOG; KOG0795; Eukaryota.
DR HOGENOM; CLU_057757_0_0_1; -.
DR OMA; VGRYDNP; -.
DR OrthoDB; 1087630at2759; -.
DR UniPathway; UPA00120; UER00203.
DR Proteomes; UP000007305; Chromosome 5.
DR ExpressionAtlas; B4FUP5; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0004106; F:chorismate mutase activity; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB.
DR GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IBA:GO_Central.
DR GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0046417; P:chorismate metabolic process; IDA:UniProtKB.
DR Gene3D; 1.10.590.10; -; 1.
DR InterPro; IPR036263; Chorismate_II_sf.
DR InterPro; IPR008238; Chorismate_mutase_AroQ_euk.
DR InterPro; IPR037039; CM_AroQ_sf_eucaryotic.
DR PANTHER; PTHR21145; PTHR21145; 1.
DR PIRSF; PIRSF017318; Chor_mut_AroQ_eu; 1.
DR SUPFAM; SSF48600; SSF48600; 1.
DR TIGRFAMs; TIGR01802; CM_pl-yst; 1.
DR PROSITE; PS51169; CHORISMATE_MUT_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amino-acid biosynthesis; Aromatic amino acid biosynthesis;
KW Cytoplasm; Isomerase; Reference proteome.
FT CHAIN 1..253
FT /note="Chorismate mutase 2, cytosolic"
FT /id="PRO_0000446625"
FT DOMAIN 2..253
FT /note="Chorismate mutase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00516"
FT CONFLICT 130
FT /note="G -> A (in Ref. 3; ACF79094)"
FT /evidence="ECO:0000305"
FT HELIX 11..32
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:6H3P"
FT STRAND 47..50
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 53..67
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 108..117
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 120..123
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 132..151
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 153..163
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 165..173
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 177..184
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 187..205
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 218..220
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 225..234
FT /evidence="ECO:0007829|PDB:6H3P"
FT HELIX 236..248
FT /evidence="ECO:0007829|PDB:6H3P"
FT TURN 249..252
FT /evidence="ECO:0007829|PDB:6H3P"
SQ SEQUENCE 253 AA; 28326 MW; BC7AE58682F928E9 CRC64;
MDAAGGDQLS LAAVRDALVR LEDSVVFALI ERARHPRNAP AYAPAATAGE HSLVEFFVRE
AEALNAKAGH YQKPEDVPFF PQDLPSPLFP TKPSPKVLHP FASLVTVNDA IWKMYFDELL
PLFTVDGDDG SYAQTVALDL ACLQVLSQRI HIGKYVAEVK FKDAPQEYSR LIKEKDSNSL
MDMLTFKAVE EKVKKRVEKK ARTFGQNVTL DDNATAGDSE CKVDPKVLSK LYDQWVMPLT
KDVEVEYLLR RLD
