ID   CM32_CONTE              Reviewed;          71 AA.
AC   Q9BPI0;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=TxMLKM-021 {ECO:0000303|PubMed:23031820, ECO:0000312|EMBL:AAG60372.1};
DE   AltName: Full=Conotoxin 2 {ECO:0000305};
DE   Flags: Precursor;
OS   Conus textile (Cloth-of-gold cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Cylinder.
OX   NCBI_TaxID=6494;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA   Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT   "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL   Mol. Biol. Evol. 18:120-131(2001).
RN   [2]
RP   PROTEIN SEQUENCE OF 55-70, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   AMIDATION AT GLU-70.
RC   TISSUE=Venom;
RX   PubMed=19380747; DOI=10.1073/pnas.0900745106;
RA   Ueberheide B.M., Fenyo D., Alewood P.F., Chait B.T.;
RT   "Rapid sensitive analysis of cysteine rich peptide venom components.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:6910-6915(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND AMIDATION AT
RP   GLU-70.
RC   TISSUE=Venom;
RX   PubMed=22709442; DOI=10.1021/pr300312h;
RA   Bhatia S., Kil Y.J., Ueberheide B., Chait B.T., Tayo L., Cruz L., Lu B.,
RA   Yates J.R. III, Bern M.;
RT   "Constrained de novo sequencing of conotoxins.";
RL   J. Proteome Res. 11:4191-4200(2012).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, POSITION IN VENOM
RP   DUCT, AND AMIDATION AT GLU-70.
RC   TISSUE=Venom;
RX   PubMed=23031820; DOI=10.1016/j.toxicon.2012.09.013;
RA   Dobson R., Collodoro M., Gilles N., Turtoi A., De Pauw E., Quinton L.;
RT   "Secretion and maturation of conotoxins in the venom ducts of Conus
RT   textile.";
RL   Toxicon 60:1370-1379(2012).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19380747}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct. Is mostly present the
CC       middle of the duct (part 3, 85%) and just after (part 4, 11%).
CC       {ECO:0000305|PubMed:19380747, ECO:0000305|PubMed:23031820}.
CC   -!- DOMAIN: The cysteine framework is III (CC-C-C-CC). Classified in the M-
CC       1 branch, since 1 residue stands between the fourth and the fifth
CC       cysteine residues. {ECO:0000305}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:19380747}.
CC   -!- MASS SPECTROMETRY: Mass=1722.553; Mass_error=0.02; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:19380747};
CC   -!- SIMILARITY: Belongs to the conotoxin M superfamily. {ECO:0000305}.
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DR   EMBL; AF214944; AAG60372.1; -; mRNA.
DR   AlphaFoldDB; Q9BPI0; -.
DR   ConoServer; 631; TxMLKM-021 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   Pfam; PF02950; Conotoxin; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..54
FT                   /evidence="ECO:0000269|PubMed:19380747"
FT                   /id="PRO_0000371273"
FT   PEPTIDE         55..70
FT                   /note="TxMLKM-021"
FT                   /evidence="ECO:0000269|PubMed:19380747"
FT                   /id="PRO_0000371274"
FT   MOD_RES         70
FT                   /note="Glutamic acid 1-amide"
FT                   /evidence="ECO:0000269|PubMed:19380747,
FT                   ECO:0000269|PubMed:22709442, ECO:0000269|PubMed:23031820"
FT   DISULFID        56..68
FT                   /evidence="ECO:0000250|UniProtKB:Q5EHP3"
FT   DISULFID        57..66
FT                   /evidence="ECO:0000250|UniProtKB:Q5EHP3"
FT   DISULFID        62..69
FT                   /evidence="ECO:0000250|UniProtKB:Q5EHP3"
SQ   SEQUENCE   71 AA;  8074 MW;  B8841CCD05FD61CC CRC64;
     MLKMGVVLFI FLVLFPLATL QLDADQPVER YAENKQLLNT DERREIILSA LRTRVCCPFG
     GCHELCQCCE G