ID   CM3F_CONPU              Reviewed;          24 AA.
AC   P60245;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2004, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Psi-conotoxin PIIIF;
OS   Conus purpurascens (Purple cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Chelyconus.
OX   NCBI_TaxID=41690;
RN   [1]
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, MASS SPECTROMETRY, STRUCTURE BY NMR,
RP   HYDROXYLATION AT PRO-2; PRO-3 AND PRO-14, AMIDATION AT LYS-24, DISULFIDE
RP   BONDS, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=12767216; DOI=10.1021/bi0272757;
RA   Van Wagoner R.M., Jacobsen R.B., Olivera B.M., Ireland C.M.;
RT   "Characterization and three-dimensional structure determination of psi-
RT   conotoxin PIIIF, a novel noncompetitive antagonist of nicotinic
RT   acetylcholine receptors.";
RL   Biochemistry 42:6353-6362(2003).
CC   -!- FUNCTION: Psi-conotoxins act on postsynaptic membranes, and act as non-
CC       competitive antagonist of nicotinic acetylcholine receptors (nAChR). Is
CC       less toxic than Psi-conotoxin PIIIE. {ECO:0000269|PubMed:12767216}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12767216}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:12767216}.
CC   -!- DOMAIN: The cysteine framework is III (CC-C-C-CC). Classified in the M-
CC       4 branch, since 4 residues stand between the fourth and the fifth
CC       cysteine residues. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=2667.68; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12767216};
CC   -!- SIMILARITY: Belongs to the conotoxin M superfamily. {ECO:0000305}.
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DR   PDB; 1JLP; NMR; -; A=1-24.
DR   PDBsum; 1JLP; -.
DR   AlphaFoldDB; P60245; -.
DR   SMR; P60245; -.
DR   ConoServer; 1594; PIIIF.
DR   EvolutionaryTrace; P60245; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0035792; C:host cell postsynaptic membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylcholine receptor inhibiting toxin; Amidation;
KW   Direct protein sequencing; Disulfide bond; Hydroxylation;
KW   Ion channel impairing toxin; Neurotoxin; Postsynaptic neurotoxin; Secreted;
KW   Toxin.
FT   PEPTIDE         1..24
FT                   /note="Psi-conotoxin PIIIF"
FT                   /evidence="ECO:0000269|PubMed:12767216"
FT                   /id="PRO_0000044498"
FT   MOD_RES         2
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:12767216"
FT   MOD_RES         3
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:12767216"
FT   MOD_RES         14
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:12767216"
FT   MOD_RES         24
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:12767216"
FT   DISULFID        4..16
FT                   /evidence="ECO:0000269|PubMed:12767216,
FT                   ECO:0007744|PDB:1JLP"
FT   DISULFID        5..21
FT                   /evidence="ECO:0000269|PubMed:12767216,
FT                   ECO:0007744|PDB:1JLP"
FT   DISULFID        10..22
FT                   /evidence="ECO:0000269|PubMed:12767216,
FT                   ECO:0007744|PDB:1JLP"
FT   TURN            14..18
FT                   /evidence="ECO:0007829|PDB:1JLP"
SQ   SEQUENCE   24 AA;  2609 MW;  6DE39416A9308C8A CRC64;
     GPPCCLYGSC RPFPGCYNAL CCRK