ID   CM4C_CONPE              Reviewed;          41 AA.
AC   Q9BP55;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Mu-conotoxin pn4c {ECO:0000303|PubMed:28831846};
DE   AltName: Full=Mu-conotoxin-like PnMMSK-D013 {ECO:0000312|EMBL:AAG60513.1};
DE   Flags: Precursor; Fragment;
OS   Conus pennaceus (Feathered cone) (Conus episcopus).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Darioconus.
OX   NCBI_TaxID=37335;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=11158371; DOI=10.1093/oxfordjournals.molbev.a003786;
RA   Conticello S.G., Gilad Y., Avidan N., Ben-Asher E., Levy Z., Fainzilber M.;
RT   "Mechanisms for evolving hypervariability: the case of conopeptides.";
RL   Mol. Biol. Evol. 18:120-131(2001).
RN   [2]
RP   PROTEIN SEQUENCE OF 25-41, MASS SPECTROMETRY, SUBCELLULAR LOCATION,
RP   FUNCTION, BIOASSAY, SYNTHESIS OF 25-41, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=28831846; DOI=10.1080/09168451.2017.1364966;
RA   Abdel-Wahab M., Miyashita M., Ota Y., Juichi H., Okabe R., Sarhan M.,
RA   Fouda M., Abdel-Rahman M., Saber S., Nakagawa Y.;
RT   "Isolation, structural identification and biological characterization of
RT   two conopeptides from the Conus pennaceus venom.";
RL   Biosci. Biotechnol. Biochem. 81:2086-2089(2017).
CC   -!- FUNCTION: Mu-conotoxins block voltage-gated sodium channels (Nav).
CC       Blocks reversibly sodium channels in molluskan neurons, but has no
CC       effect on sodium currents in bovine chromaffin cells or in rat brain
CC       synaptosomes (By similarity). Induces paralysis in mollusks
CC       (C.retripictus) (PubMed:28831846). {ECO:0000250,
CC       ECO:0000269|PubMed:28831846}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11158371}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:11158371}.
CC   -!- DOMAIN: The cysteine framework is IV (CC-C-C-C-C). {ECO:0000305}.
CC   -!- PTM: Contains 3 disulfide bonds. They are not added, since framework IV
CC       presents two different connectivities (I-V, II-III, IV-VI and I-III,
CC       II-V, IV-VI). {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1775.6; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:28831846};
CC   -!- TOXIC DOSE: PD(50) is 0.2-0.4 nmol/g when injected into the foot of the
CC       freshwater snail C.retropictus. {ECO:0000269|PubMed:28831846}.
CC   -!- SIMILARITY: Belongs to the conotoxin M superfamily. {ECO:0000305}.
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DR   EMBL; AF215092; AAG60513.1; -; mRNA.
DR   AlphaFoldDB; Q9BP55; -.
DR   ConoServer; 770; Pn4.1 precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   PROPEP          <1..24
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000404928"
FT   PEPTIDE         25..41
FT                   /note="Mu-conotoxin pn4c"
FT                   /evidence="ECO:0000269|PubMed:28831846"
FT                   /id="PRO_0000404929"
FT   UNSURE          34
FT                   /note="L or I"
FT                   /evidence="ECO:0000305|PubMed:28831846"
FT   NON_TER         1
SQ   SEQUENCE   41 AA;  4647 MW;  23FAF02B1D4FE440 CRC64;
     DQPAERMQDD ISSEHHPFFD PVKRCCKYGW TCVLGCSPCG C