ID   CMBL_DICDI              Reviewed;         255 AA.
AC   Q54MZ9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Carboxymethylenebutenolidase homolog;
DE            EC=3.1.-.-;
GN   Name=cmbl; ORFNames=DDB_G0285591;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Cysteine hydrolase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000079; EAL64633.1; -; Genomic_DNA.
DR   RefSeq; XP_638144.1; XM_633052.1.
DR   AlphaFoldDB; Q54MZ9; -.
DR   SMR; Q54MZ9; -.
DR   STRING; 44689.DDB0266728; -.
DR   ESTHER; dicdi-CMBL; Dienelactone_hydrolase.
DR   PaxDb; Q54MZ9; -.
DR   EnsemblProtists; EAL64633; EAL64633; DDB_G0285591.
DR   GeneID; 8625192; -.
DR   KEGG; ddi:DDB_G0285591; -.
DR   dictyBase; DDB_G0285591; cmbl.
DR   eggNOG; ENOG502RSRP; Eukaryota.
DR   HOGENOM; CLU_054590_7_3_1; -.
DR   InParanoid; Q54MZ9; -.
DR   OMA; FGHADND; -.
DR   PhylomeDB; Q54MZ9; -.
DR   PRO; PR:Q54MZ9; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..255
FT                   /note="Carboxymethylenebutenolidase homolog"
FT                   /id="PRO_0000331365"
FT   ACT_SITE        135
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        191
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        225
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   255 AA;  28841 MW;  D7AD3BA6152D1117 CRC64;
     MKGELIDITM KSGLKCDAYV SRPKDSQKEL KPVIFVMDAF GLRDWLYEMA DKIAEEGYFV
     VQPNFYYRIG KNIITNLEKL KSADTKDEVI CQIRTQMAKI NREETVSDVS EMFDFIDKQE
     GVRKSKEGVA IVGYCFGGGV AMRSAIAFPD IVKVVASFHA GRLAIPDDEN SIHKHLKGVK
     AECYFGHADN DQSMPLDQIH LFEKSLTEAG IKYTSEIYNN PSCAHGWVMG DTLMYNPIGS
     DKHYDELFKL LKRAL