ID   CMBL_RAT                Reviewed;         245 AA.
AC   Q7TP52;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Carboxymethylenebutenolidase homolog;
DE            EC=3.1.-.-;
DE   AltName: Full=Liver regeneration-related protein LRRG072;
GN   Name=Cmbl; ORFNames=Ab2-225;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RA   Xu C.S., Li W.Q., Li Y.C., Chai L.Q., Yuan J.Y., Yang K.J., Yan H.M.,
RA   Chang C.F., Zhao L.F., Ma H., Wang L., Wang S.F., Han H.P., Wang G.P.,
RA   Shi J.B., Rahman S., Wang Q.N., Zhang J.B.;
RT   "Liver regeneration after PH.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Cysteine hydrolase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; AY325197; AAP92598.1; -; mRNA.
DR   EMBL; BC088459; AAH88459.1; -; mRNA.
DR   RefSeq; NP_001008770.1; NM_001008770.3.
DR   RefSeq; XP_006232159.1; XM_006232097.3.
DR   AlphaFoldDB; Q7TP52; -.
DR   SMR; Q7TP52; -.
DR   STRING; 10116.ENSRNOP00000015448; -.
DR   ESTHER; ratno-CMBL; CMBL.
DR   PaxDb; Q7TP52; -.
DR   PRIDE; Q7TP52; -.
DR   Ensembl; ENSRNOT00000015448; ENSRNOP00000015448; ENSRNOG00000011260.
DR   GeneID; 310201; -.
DR   KEGG; rno:310201; -.
DR   UCSC; RGD:1306952; rat.
DR   CTD; 134147; -.
DR   RGD; 1306952; Cmbl.
DR   eggNOG; KOG3043; Eukaryota.
DR   GeneTree; ENSGT00390000000183; -.
DR   HOGENOM; CLU_054590_8_2_1; -.
DR   InParanoid; Q7TP52; -.
DR   OMA; HPSMLEK; -.
DR   OrthoDB; 1275897at2759; -.
DR   PhylomeDB; Q7TP52; -.
DR   TreeFam; TF331795; -.
DR   BRENDA; 3.1.1.45; 5301.
DR   Reactome; R-RNO-211945; Phase I - Functionalization of compounds.
DR   PRO; PR:Q7TP52; -.
DR   Proteomes; UP000002494; Chromosome 2.
DR   Bgee; ENSRNOG00000011260; Expressed in adult mammalian kidney and 19 other tissues.
DR   Genevisible; Q7TP52; RN.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR042946; CMBL.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   PANTHER; PTHR46812; PTHR46812; 1.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cytoplasm; Hydrolase; Phosphoprotein; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q96DG6"
FT   CHAIN           2..245
FT                   /note="Carboxymethylenebutenolidase homolog"
FT                   /id="PRO_0000308191"
FT   ACT_SITE        132
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        179
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        212
FT                   /evidence="ECO:0000250"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96DG6"
FT   MOD_RES         223
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96DG6"
SQ   SEQUENCE   245 AA;  27903 MW;  F3650F3D62148350 CRC64;
     MANEANPCPC DIGHRLDYGG MGQEVQVEHI KAYVTRSPVD AGKAVIVVQD IFGWQLSNTR
     YMADMIAGNG YTTIVPDFFV GQEPWDPAGD WSTFPEWLKS RNARKINREV DAVLRYLKQQ
     CHAQKIGIVG FCWGGIVVHH VMTTYPEVRA GVSVYGIIRD SEDVYNLKNP TLFIFAENDA
     VIPLEQVSIL IQKLKEHCIV NYQVKTFSGQ THGFVHRKRE DCSPADKPYI EEARRNLIEW
     LNKYI