ID   CMCH_AMYLA              Reviewed;         520 AA.
AC   Q51080;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=3'-hydroxymethylcephem-O-carbamoyltransferase;
DE            Short=3'-hydroxymethylcephem-O-CASE;
DE            Short=CCT;
DE            EC=2.1.3.-;
GN   Name=cmcH;
OS   Amycolatopsis lactamdurans (Nocardia lactamdurans).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Amycolatopsis.
OX   NCBI_TaxID=1913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7557411; DOI=10.1016/0378-1119(95)00308-s;
RA   Coque J.J.R., Perez-Llarena F.J., Enguita F.J., Fuente J.L., Martin J.F.,
RA   Liras P.;
RT   "Characterization of the cmcH genes of Nocardia lactamdurans and
RT   Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-
RT   carbamoyltransferase for cephamycin biosynthesis.";
RL   Gene 162:21-27(1995).
CC   -!- FUNCTION: Catalyzes the carbamoylation reaction in the cephamycin
CC       biosynthesis.
CC   -!- PATHWAY: Antibiotic biosynthesis; cephamycin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the NodU/CmcH family. {ECO:0000305}.
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DR   EMBL; Z21682; CAA79798.1; -; Genomic_DNA.
DR   PIR; S40256; S40256.
DR   AlphaFoldDB; Q51080; -.
DR   SMR; Q51080; -.
DR   UniPathway; UPA00183; -.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.870.20; -; 1.
DR   InterPro; IPR031730; Carbam_trans_C.
DR   InterPro; IPR038152; Carbam_trans_C_sf.
DR   InterPro; IPR003696; Carbtransf_dom.
DR   Pfam; PF16861; Carbam_trans_C; 1.
DR   Pfam; PF02543; Carbam_trans_N; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Transferase.
FT   CHAIN           1..520
FT                   /note="3'-hydroxymethylcephem-O-carbamoyltransferase"
FT                   /id="PRO_0000207854"
SQ   SEQUENCE   520 AA;  57143 MW;  E3B704E9C907F802 CRC64;
     MLIVAFKPGH DGAVAAIGDR RLLYSLESEK DSRPRYSPIL ATTVLDLAER LGEVPDVVAL
     GGWSDLRPNR ISYTGAGYSG IEEPTVTTSR FFGKEVKFFS STHERSHIYM ALGMAPRDDS
     PVQTVLVWEG DVGAFYVIDG HQRITRKVQV MSGPGARYSF LFGLADPTFP TTGGKPRLND
     AGKLMALAAF GDSADADADI THVVERILKQ DSMYPAPKGE YRDSVLYNAG VESPECKIAA
     ALLTERLFET FAEVARQEMP EGSPLYISGG CGLNCDWNSL WAQLGHFSSV FVAPCTNDSG
     SALGTAIDAL TTFTGDPHVD WSVYSGLEFV TDTQPDPARW TSRPLEHDEL SGALAGGRVV
     AWVQGRWEIG PRALCNRSLL AEPFGAVTRD RLNEIKQRED YRPIAPVCRV EDLGKVFHED
     FEDPYMLYFR RVRESSGLRA VTHVDGSARV QTVRDSGNPQ MHRLLSAFAA QRGVGVLCNT
     SLNFNGEGFI NRMSDLVLYC ESRGISDMVV GDTWYQRAEG