ID   CMCH_STRCL              Reviewed;         521 AA.
AC   O85728;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=3'-hydroxymethylcephem-O-carbamoyltransferase;
DE            Short=3'-hydroxymethylcephem-O-CASE;
DE            Short=CCT;
DE            EC=2.1.3.-;
GN   Name=cmcH;
OS   Streptomyces clavuligerus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL
RC   3585 / VKM Ac-602;
RX   PubMed=9696752; DOI=10.1128/jb.180.16.4068-4079.1998;
RA   Alexander D.C., Jensen S.E.;
RT   "Investigation of the Streptomyces clavuligerus cephamycin C gene cluster
RT   and its regulation by the CcaR protein.";
RL   J. Bacteriol. 180:4068-4079(1998).
CC   -!- FUNCTION: Catalyzes the carbamoylation reaction in the cephamycin
CC       biosynthesis.
CC   -!- PATHWAY: Antibiotic biosynthesis; cephamycin C biosynthesis.
CC   -!- SIMILARITY: Belongs to the NodU/CmcH family. {ECO:0000305}.
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DR   EMBL; AF073897; AAC32493.1; -; Genomic_DNA.
DR   RefSeq; WP_003952499.1; NZ_CP032052.1.
DR   AlphaFoldDB; O85728; -.
DR   SMR; O85728; -.
DR   STRING; 443255.SCLAV_4205; -.
DR   GeneID; 61469714; -.
DR   eggNOG; COG2192; Bacteria.
DR   OMA; GHHFGPY; -.
DR   OrthoDB; 509293at2; -.
DR   BioCyc; MetaCyc:MON-13420; -.
DR   UniPathway; UPA00183; -.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.870.20; -; 1.
DR   InterPro; IPR031730; Carbam_trans_C.
DR   InterPro; IPR038152; Carbam_trans_C_sf.
DR   InterPro; IPR003696; Carbtransf_dom.
DR   Pfam; PF16861; Carbam_trans_C; 1.
DR   Pfam; PF02543; Carbam_trans_N; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Transferase.
FT   CHAIN           1..521
FT                   /note="3'-hydroxymethylcephem-O-carbamoyltransferase"
FT                   /id="PRO_0000207855"
SQ   SEQUENCE   521 AA;  57370 MW;  A80C078999B137B1 CRC64;
     MLVVAFKPGH DGAVAAIDDR RLLYSLESEK DSRPRYSTLL PTTFLDIAER LGAIPDVVAL
     GGWADLRPRG VVYTGAGYEG TQEPTVTTSR FFGKEVKFFT STHERSHIYM ALGMAPKDGA
     PLKSVLVWEG DVGAFYLVDS EHRIIRTIPV MTGPGARYSF LFGLADPTFP DTGGKPRLND
     AGKLMALAAF GDSADASPDI RHVVERVLKQ DSMYPAPKAE YRDSVLHNAG VESEECKIAA
     ALLTERLFET FAEVARRELP EGTPLYISGG CGLNCDWNSQ WAQLGHFSSV FVAPCTNDSG
     SALGTAIDAL TTFTGDPHID WNVYSGLEFV HDTRPDPARW ESVPLDHAAL SSALAAGRVV
     AWVQGRWEIG PRALGNRSLL AEAFSSASRD RLNTVKMRED YRPIAPVCRV EDLGKVFHED
     FEDPHMLYFR RVREESGVRA VTHVDGSARC QTVTGTSNPE LHRLLSVFAQ GQGLGVLCNT
     SLNFNGDGFI NRMSDLVRYC EWREIQDMVV GDTWYRRIAG S