CMFB_DICDI
ID CMFB_DICDI Reviewed; 510 AA.
AC Q9U641; Q54HX4;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Conditioned medium factor receptor 1;
GN Name=cmfB; Synonyms=cmfr1; ORFNames=DDB_G0289157;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION,
RP PROTEIN SEQUENCE OF 57-81; 141-154 AND 417-425, DEVELOPMENTAL STAGE,
RP FUNCTION, AND TOPOLOGY.
RX PubMed=10567429; DOI=10.1074/jbc.274.48.34476;
RA Deery W.J., Gomer R.H.;
RT "A putative receptor mediating cell-density sensing in Dictyostelium.";
RL J. Biol. Chem. 274:34476-34482(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP TOPOLOGY, AND FUNCTION.
RX PubMed=12070170; DOI=10.1074/jbc.m204539200;
RA Deery W.J., Gao T., Ammann R., Gomer R.H.;
RT "A single cell density-sensing factor stimulates distinct signal
RT transduction pathways through two different receptors.";
RL J. Biol. Chem. 277:31972-31979(2002).
RN [4]
RP IDENTIFICATION.
RX PubMed=16469056; DOI=10.1111/j.1462-5822.2005.00633.x;
RA Farbrother P., Wagner C., Na J., Tunggal B., Morio T., Urushihara H.,
RA Tanaka Y., Schleicher M., Steinert M., Eichinger L.;
RT "Dictyostelium transcriptional host cell response upon infection with
RT Legionella.";
RL Cell. Microbiol. 8:438-456(2006).
RN [5]
RP IDENTIFICATION.
RX PubMed=16782457; DOI=10.1016/s0070-2153(05)73004-0;
RA Mahadeo D.C., Parent C.A.;
RT "Signal relay during the life cycle of Dictyostelium.";
RL Curr. Top. Dev. Biol. 73:115-140(2006).
RN [6]
RP IDENTIFICATION.
RX PubMed=17481898; DOI=10.1016/j.cub.2007.04.029;
RA Bakthavatsalam D., Brazill D., Gomer R.H., Eichinger L., Rivero F.,
RA Noegel A.A.;
RT "A G protein-coupled receptor with a lipid kinase domain is involved in
RT cell-density sensing.";
RL Curr. Biol. 17:892-897(2007).
CC -!- FUNCTION: Receptor for cmfA, that appears to mediate the G-independent
CC cmfA signal transduction. {ECO:0000269|PubMed:10567429,
CC ECO:0000269|PubMed:12070170}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in vegetative and early developing cells
CC and then begin to disappear as the cells aggregate.
CC {ECO:0000269|PubMed:10567429}.
CC -!- DISRUPTION PHENOTYPE: Null cells show a 50% decrease of the CMF binding
CC and a loss of CMF-induced G protein-independent gene expression.
CC {ECO:0000269|PubMed:10567429}.
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DR EMBL; AF193857; AAF05771.1; -; mRNA.
DR EMBL; AAFI02000130; EAL62857.1; -; Genomic_DNA.
DR RefSeq; XP_636360.1; XM_631268.1.
DR AlphaFoldDB; Q9U641; -.
DR SMR; Q9U641; -.
DR STRING; 44689.DDB0191095; -.
DR PaxDb; Q9U641; -.
DR EnsemblProtists; EAL62857; EAL62857; DDB_G0289157.
DR GeneID; 8626989; -.
DR KEGG; ddi:DDB_G0289157; -.
DR dictyBase; DDB_G0289157; cmfB.
DR eggNOG; ENOG502RST9; Eukaryota.
DR HOGENOM; CLU_024648_5_2_1; -.
DR InParanoid; Q9U641; -.
DR OMA; SLCDPFL; -.
DR PhylomeDB; Q9U641; -.
DR PRO; PR:Q9U641; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR Pfam; PF01494; FAD_binding_3; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Receptor; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..510
FT /note="Conditioned medium factor receptor 1"
FT /id="PRO_0000368226"
FT TOPO_DOM 1..36
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 510 AA; 56497 MW; 3040E0A426686C34 CRC64;
MDSKYIQKTL SAITEQITKN AAVQKVLDNK FVKEHKYAAA AATVGLGVVA ATTIVKAVNC
EGKRYNYDAI PNLSFDDESI YDVATIGAGP SGSVLGYYLA REGRKVALLE KKVFPRDKYC
GDAVATMAQD ILREMGVMKE LVDEDLGHFA QNGGFVSPNG NSFIGNSAKE LKRDAKYNRG
AVIAVKRIVL DEKVAKAAKR MGADLKENTT VENATFDRST GVWTINCVDS EDNTKKIVYR
ARVLVCADGS PSNAARQLGY VHTEPNGICS RAYVKNNTTF RYDGVVFYPP SLLPGYCAII
REARDELNYL AYIIPGGKVT NDDLSKYHHQ YMTEDPFISA ALGPNPDIER MKAAPLRLGG
IKKSYDDHLL IVGDAAGFID PLTGEGIQYA MEGSRLASLA LIQAFNERDL SHQSLKRYQD
FWMAKFGHEF SMSMTMSLFL YRFPIVLDAA ASLIEKRGSR FLAEWAAVMT GVKPKTWFLR
PDVGPLIVLE IFGECFRRVF QGKKQIKKLD
