CMF_DICDI
ID CMF_DICDI Reviewed; 699 AA.
AC P34090; Q553Y6; Q869Y2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Conditioned medium factor;
DE Short=CMF;
DE AltName: Full=Density-sensing factor;
DE Flags: Precursor;
GN Name=cmfA; ORFNames=DDB_G0275007;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 58-699, AND PROTEIN SEQUENCE OF 406-438;
RP 448-460 AND 692-698.
RC STRAIN=AX4;
RX PubMed=1547939; DOI=10.1101/gad.6.3.390;
RA Jain R., Yuen I.S., Taphouse C.R., Gomer R.H.;
RT "A density-sensing factor controls development in Dictyostelium.";
RL Genes Dev. 6:390-400(1992).
CC -!- FUNCTION: Involved in cell density sensing and might synchronize the
CC onset of development by triggering aggregation when a majority of the
CC cells in a given area have starved.
CC -!- PTM: N- and O-glycosylated.
CC -!- PTM: The N-terminus is blocked.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA77749.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA77749.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000013; EAL69784.1; -; Genomic_DNA.
DR EMBL; Z11691; CAA77749.1; ALT_SEQ; mRNA.
DR PIR; A42138; A42138.
DR PIR; S24482; S24482.
DR RefSeq; XP_643825.1; XM_638733.1.
DR AlphaFoldDB; P34090; -.
DR STRING; 44689.DDB0191097; -.
DR PaxDb; P34090; -.
DR ABCD; P34090; 10 sequenced antibodies.
DR EnsemblProtists; EAL69784; EAL69784; DDB_G0275007.
DR GeneID; 8619872; -.
DR KEGG; ddi:DDB_G0275007; -.
DR dictyBase; DDB_G0275007; cmfA.
DR eggNOG; ENOG502RYH8; Eukaryota.
DR HOGENOM; CLU_413280_0_0_1; -.
DR InParanoid; P34090; -.
DR OMA; DESMRMG; -.
DR PRO; PR:P34090; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005615; C:extracellular space; IDA:dictyBase.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0045597; P:positive regulation of cell differentiation; IDA:dictyBase.
DR GO; GO:0010468; P:regulation of gene expression; IDA:dictyBase.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Reference proteome; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..699
FT /note="Conditioned medium factor"
FT /id="PRO_0000089883"
FT REGION 680..699
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 283
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 346
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 430
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 130
FT /note="N -> M (in Ref. 3; CAA77749)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="S -> T (in Ref. 3; CAA77749)"
FT /evidence="ECO:0000305"
FT CONFLICT 290
FT /note="Y -> H (in Ref. 3; CAA77749)"
FT /evidence="ECO:0000305"
FT CONFLICT 623..624
FT /note="YT -> LS (in Ref. 3; CAA77749)"
FT /evidence="ECO:0000305"
FT CONFLICT 699
FT /note="W -> C (in Ref. 3; CAA77749)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 699 AA; 76555 MW; 294B3ECA86C96055 CRC64;
MRLLLLLILI ITINFSYGVL TPKNLAGEAS EFGSFNIPNP MDVAQSSDSS LFGISMTQSQ
LKNSFEWSGV VPVDSEEEFT LTFFSSFPLS EFKVEASPKT SLSSSKSESE HKSKFYKVIK
SIYQTPTVTN GSFGIDGATT PSFSLTWDKP VVGDWNVVIT ASSSLRKNEK FQKMVEDSTP
QLLMLVQNPS DTHIYSYVSS YNNLFTGQKV SVLAMLHKKS EFIKKSSANR PLNWKPSPIL
LSDVSAEMIL GLPDGSKETI PMFDDGLHDD EQANDGLFGG YINVSELGNY DLQVVYKGSQ
NGNGVIRSNQ HLIPITSQYL ELTGEVQSVQ DGDANLNIYF IVNSPNQTTV DQTPVHVYSE
VYGTDDDGKK VAIAWVAGVT SAQPIQGSTT TFALSAVLNE RWIAKVGATA PFFVKNVQVS
DLDTFIPLSN TTSTSNVKMV GEYKDVRTIV HSPPLHEITK EMRDGKMPKE LADRIGKSTG
NGKLILTHGY CSEGVWPIED FENSVEFQDF NQNRGNDEFA QILANFGSQY TDGFSLVAHS
QGGNAALHLV TFYFSGLDLS QKLEGRVIQS MGTPYQGTAL AGTWASIGSA VGVGCSANDD
LTVDGAALWL KSIPADKRAL VYYTTTQYST GSLINYCNLA SNAVLEWPND GVVDNEHTPL
EGGVYLNNFK DWCHSDGMHS PQQTTNTEYN KEMSSNSVW
